Paper Chromatography

What is paper chromatography?
1. is a technique of using a solvent to separate a mixture into its components for identification purposes and determining of purity.
  1. very useful method to separate and identify components in a sample like dyes in inks, poison in drugs etc.
Process: 1.a pencil line is drawn across the chromatography paper to indicate the starting position of mixture spots.
1. 2. important to use a pencil because inks will sumuge
  1. 3. Paper dipped into a solvent
    1. mixture dissolve in the solvent and moves up the paper
2. results:
How does it work?
1. http://www.youtube.com/watch?v=PN5YiY0Wm6o
  1. http://www.youtube.com/watch?v=gpFb635N2wo
How to identify colourless substances?
1. Apply a locating agent on the chromatogram
  1. agents react with the substances to produce a coloured substance
applications of chromatography
1. separate and identify dyes and drugs
  1. identify chemicals sprayed on vegetables
    1. determine purity of a sample
Advantages of chromatography
1. allow analysis if a tiny amount of chemicals
  1. sensitive and highly reproducible
    1. allow analysis of many samples in a time.
How do we interpret the result of chromatography?
1. If the chromatogram shows food colouring is a mixture of 2 dyes, It is not pure.
  1. But is there is only one spot, it means it is pure,

Resource summary

	Created by sarahyeoh almost 11 years ago