6571949
Description
Mind Map by cara brand, updated more than 1 year ago
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Created by cara brand
about 8 years ago
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Experimental Methods: Bio110-2016
- Nucleotides
- Do I want to know where they are made?
- Yes
- Pulse experiment... I know what
radioactive precursor to use, right?
- Autoradiography
- Do I want to visualize the cell?
- Microscopy... I know which kind I
should be using, right? And how to
prepare my cells or tissues?
- Light or Flourescent Microscopy
- Electron Microscopy
- Light or Flourescent Microscopy
- Microscopy... I know which kind I
should be using, right? And how to
prepare my cells or tissues?
- Do I want to differentiate
between locations in the cell
- Cell fractionization... I know which cell
components separate at different
speeds, right?
- Cell fractionization... I know which cell
components separate at different
speeds, right?
- Do I want to visualize the cell?
- Autoradiography
- Pulse experiment... I know what
radioactive precursor to use, right?
- No, I want to know
where they're going?
- Pulse + Chase experiment
- Pulse + Chase experiment
- I just want to label them!
- Yes
- Do I want to know where they are made?
- Proteins
- Am I interested in a specific protein?
- No, I'm interested in them all
- Yes!
- Well then I need an Antibody...
I know how to make one, right?
- Do I want to visualize where the
protein localizes in the cell?
- Fluorescent labeled antibody?
- Gold labeled antibody?
- Fluorescent labeled antibody?
- Do I want to test which tissues, or individuals, or
samples contain my protein?
- Studying Tissues:
Harvest Sample
- Studying cell
components:
fractionization
- Purify proteins. Use SDS
- Gel Electrophoresis... before I visualize my
protein of interest, I have to size separate all
of them
- Western Blot
- Western Blot
- Gel Electrophoresis... before I visualize my
protein of interest, I have to size separate all
of them
- Purify proteins. Use SDS
- Studying individuals:
sample individuals
- Studying Tissues:
Harvest Sample
- Do I want to visualize where the
protein localizes in the cell?
- Well then I need an Antibody...
I know how to make one, right?
- No, I'm interested in them all
- Am I interested in a specific protein?
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