Criado por kerryrandfield
mais de 9 anos atrás
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Questão | Responda |
THREE DOMAINS OF LIFE | > bacteria > archaea > eukaryotes |
WHY MICROORGANISMS ARE USEFUL FOR RESEARCH AND INDUSTRY | > easily cultivated > reproduce and grow quickly > food substrate is often cheap > produce many different useful products > metabolism is easily manipulated |
SELECTIVE MEDIUM | a medium designed to suit the growth requirements of a specific microorganism |
GENERATION TIME / DOUBLING TIME | time between cell divisions / time for population to double |
LAG PHASE | > no (or very little) increase in cell number > cells are adjusting to the new medium > synthesising new enzymes to break down new substrates |
LOG / EXPONENTIAL PHASE | cells divide and numbers increase exponentially enzymes are being induced to break down substrate |
STATIONARY PHASE | growth rate slows death rate = birth rate microbes run out of nutrient/space and toxic metabolites build up |
DEATH PHASE | death rate > birth rate toxic waste products build up |
PRIMARY METABOLISM | organisms break down substrates to produce metabolites essential for growth, development, reproduction |
SECONDARY METABOLISM | breaking down substrate to produce metabolites giving an ecological advantage to microbe |
DESIRED CHARACTERISTICS OF A STRAIN | > genetic stability > ability to grow on low cost nutrients > ability to vastly overproduce target compound > ability to allow easy harvesting of the product |
MUTAGENESIS | the creation of mutations |
GENES THAT CAN BE INTRODUCED TO MICROORGANISMS | > amplify specific steps to increase yield of target compound > causes cell to secrete product (easily recovered) > makes them unable to survive in external environment (safety mechanism) |
VECTOR | transfers DNA from genome of one organism to that of another (often a plasmid) |
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