Questão | Responda |
Spore-forming aerobic gram positive bacilli | Bacillus spp. |
Non-spore forming aerobic gram positive bacilli | Listeria Erysipelothrix Corynebacterium |
Are all Bacillus species found in soil? | YES! |
Is Bacillus catalase positive or negative? | POSITIVE! |
Name the most common disease you can get from Bacillus cereus. | Food poisoning mediated by toxin (Pwede ding eye & wound infection kung opportunistic) |
Enumerate the two forms of food poisoning | Diarrheal (24 hours) caused by eating meat & Emetic (10 hours) caused by fried rice Note: Both of them are self limiting |
Type of hemolysis on agar plate produced by Bacillus cereus | Beta hemolysis |
How can you acquire anthrax? | Through contact with anthrax infected animals or animal products. There is no human-to-human spread. |
The causative agent of woolsorter's disease | Bacillus anthracis |
Three forms of anthrax | Cutaneous Inhalation Gastrointestinal |
Describe the disease progression of cutaneous anthrax | (PVU) Starts as a PAPULE VESICULAR Depressed black necrotic ULCER *eschar* |
Describe the pathology of inhalation anthrax | Begins as "viral-like" Respiratory symptoms, fever, fatigue, myalgia leading to hypoxia Possibility of rhinorrhea/runny nose |
This form of anthrax is sometimes seen as oropharyngeal ulcerations with cervical adenopathy and fever | GI anthrax |
Give some complications of anthrax | Meningitis (5%) Coma, Death (1-6 days after exposure) |
Does recovery from anthrax confer infection? | Fortunately, yes! |
Give some antibiotics used after anthrax exposure | Ciprofloxacin Tetracycline |
Describe the characteristic appearance of Bacillus colonies | Medusa head appearance Non-hemolytic Catalase positive |
Bacteria associated with meat processors and veterinarians | Listeria monocytogenes |
Is congenital transmission possible for Listeriosis? | Sadly, yes. :( |
Fill in the data for Listeria monocytogenes Appearance on Gram stain: Oxygen requirements: Spore formation: Acid-fast: Temperature requirements: Hemolytic pattern: Motility pattern: | GP short rods or coccobacilli Aerobic Non-spore-forming Not acid fast either Grows at 4 degrees Beta hemolysis Umbrella pattern |
Listeriosis is predominantly food-borne. Yes or No? | Yes! |
Agent of swine erysipelas and human erysipeloid | Erysipelothrix rhusiopathiae |
Small GP bacilli Singly, short chains, filaments Aerobic or FA Nonmotile Catalase (-) | Erysipelothrix rhusiopathiae |
Erysipelothrix is resistant to what kind of environment? | Cold and alkaline |
Cutaneous human erysipelothrix infection consisting of localized cellulitis after acquisition through the skin | Erysipeloid (-pelas for animals) (-peloid for man) *sounds like keloid* |
Specimen of choice for diagnosis of erysipelothrix infection | Skin biopsy |
Medium for cultivation of Erysipelothrix | SBA with 5% to 10% CO2 |
Triple Sugar Iron Agar Slant for Erysipelothrix: H2s Positive or Negative? | Positive! |
Gram positive bacilli resembling "Chinese letters" and have a characteristic "dry colonial morphology" | Corynebacterium spp. |
Hallmark of corynebacterium disease | Adherent membrane of the tonsils, pharynx, nose |
5 Complications of corynebacterium infection | (5 top organs) Heart: myocarditis Kidney and Liver: inflammation Brain: peripheral neuropathy Lungs: airway obstruction DEATH |
How does corynebacterium invade the body? | Colonizing and proliferating in the throat, creating pseudomembrane and producing an EXOTOXIN leading to cell death |
Culture medium selective for Corynebacteirum diphtheriae (black to gray) | Cystine-Tellurite Medium (CTBA) |
Culture medium differential for Corynebacteirum diphtheriae (black with brown halos) | Tinsdale Agar |
Biochemical characteristics of Corynebacteirum diphtheriae | CUN :: PNP Catalalse (+) Urease (-) Nitrate (+) |
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