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Nós do fluxograma
- DISAGGREGATION OF EXPLANTS
- FINE DISSECTION
- MECHANICAL DISAGGREGATION
- COLD TRYPSIN
- WARM TRYPSIN
- COLLAGENASE
- DISPERSED PRIMARY CULTUTURE
- SUBCULTURE
- PRIMARY EXPLANT
- TRANSFER
- 2ND EXPLANT
- OVER NIGHT INCUBATION
- LONG INCUBATION
- CENTRIFUGE
- CELL LINE
- OUTGROWTH
- RESUSPEND AND SEED
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