2979748
Histology
- Light microscopy
- Formalyn,
paraffin
embedding
- Formalyn,
paraffin
embedding
- TEM
- Shows interior of the
Cell
- Glutaraldehyde and
osmium tetroxide
- Glutaraldehyde and
osmium tetroxide
- Shows interior of the
Cell
- SEM
- Specimen is sputter coated with silver
- glutaraldehyde
- glutaraldehyde
- Specimen is sputter coated with silver
- Fluorescent Microscopy
- Immunohistochemistry
- substrate or flourescence
- substrate or flourescence
- Immunohistochemistry
- Hematoxylin and Eosin
- Most Common
- Hematoxylin
stains Nucleic
acids & ER Blue
- Neurons - Basophilic Nissl bodies in cytoplasm (rER)
- Neurons - Basophilic Nissl bodies in cytoplasm (rER)
- Eosin stains
(acidic)
mitochondria Hemoglobin, Elastic Fibers
red/pink
- Most Common
- Periodic acid-Schiff (PAS)
- Stain for glycoproteins Sugar
- MicroVilli (Glycocalyx)
- MicroVilli (Glycocalyx)
- Basal lamina (Laminin) Red / Pink / Magenta
- Type IV Collagen
- Type IV Collagen
- Stain for glycoproteins Sugar
- Trichrome Blue
- Collagen Type I
- Collagen Type I
- Silver Stain
- Reticular Fibers
- Type III
- Brown / Black
- Type III
- Nerve Fibers
- Brown / Black
- Brown / Black
- Reticular Fibers
- Bright Field
- Light beam passes through stained specimen
- Light beam passes through stained specimen
- Hyaline (grape soda)
- Type II - Basophilic
- Aggrecan Hyaluronic Acid
- Chondroitin Sulfate
- A lot of smooth extra cellular matrix between cells
- Type II - Basophilic
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