LARGE-SCALE BIOAUGMENTATION OF SOILS
CONTAMINATED WITH PETROLEUM HYDROCARBONS
USING A MIXED MICROBIAL CONSORTIUM
Soil
contamination
eroded
method used was
bioaugmentation
treatment
addition of 22
bacterial strains
Laboratory experiments Preliminary
laboratory experiments were carried out to
test the efficacy of the bacterial consortium
used in the bioaugmentation of soil sludge
Field Experiments Approximately
250 metric tons of materials were
excavated from the reserve of aged
and degraded TPH contaminated
soil and transported to a specially
prepared pit with a screen.
resultados que se obtuvieron fueron La biorremediación
del suelo contaminado en estudios de laboratorio. La
adición del consorcio bacteriano resultó en una
degradación del 100% del TPH presente en el suelo
conclusion This study highlights the importance of an
adapted indigenous consortium to improve the degradation
of hydrocarbons and confirms bioremediation as an
adequate strategy for the treatment of soils contaminated
with hydrocarbons on a commercial scale.
DECONTAMINATION OF POLYCYCLIC AROMATIC
HYDROCARBONS IN PEAS SEEDS BY GAMMA
IRRADIATION
Soil contamination
método que se utiliza
es semillas de
guisantes
seeds were exposed to gamma irradiation at doses of 0,
1, 5, 10 and 15 kGy. PAHs were extracted and their
concentrations in each dose were determined by means
of a high-performance liquid chromatography equipped
with a fluorescent detector.
Results show that PAH concentrations decreased as
the gamma irradiation dose increased, since the 15
kGy gamma irradiation dose reduced the total PAH in
the pea seeds studied by approximately 96%
These findings are of great importance and clearly
indicate the practical possibility of gamma irradiation in
the decontamination of pea seeds from PAH without
changing their chemical content.
structural dynamics of microbial
communities in sediments of tropical
estuaries contaminated with
polycyclic aromatic hydrocarbons
subjected to simulated aerobic
biotreatment
Environmental pollution by polycyclic
aromatic hydrocarbons (PAH
Microbial community
method
Sediment samples were collected from the
laguana de lagos
Results. Biodegradation of all PAHs except benzo (a)
anthracene was detected in all sediments in 2 weeks The
degradation of benzo (a) anthracene was not apparent
until week 5
Microbial degradation is a key process that affects the
fate of hap in soils and sediments, and can be carried
out by aerobic or anaerobic bacteria, however, of
these two groups, the range of hap degraded by
aerobes is much higher than that of the anaerobic.
application of the landfarming
technique for the remediation of
soils contaminated with
hydrocarbons
damage to water sources, soils, air,
fauna and vegetation
Bioremediation .
This technique consists of excavating the
contaminated soils, spreading them over a
sufficiently wide area and stimulating the
variables of incidence in the process to promote
the activity of the microorganisms in charge of
degrading the hydrocarbons
Research shows the existence in
nature of microbial agents capable of
The biological treatment of contaminated soils involves
the use of microorganisms and / or plants for the
degradation of organic pollutants. Biological activity alters
the molecular structure of the pollutant and the degree of
alteration determines whether biotransformation or
mineralization has occurred
The most important chemical factor in Bioremediation
is the molecular structure of the pollutant, how it
affects its chemical and physical properties and its
ability to be biodegraded.
BIODEGRADATION OF POLYCYCLIC
AROMATIC HYDROCARBONS BY MICROBIAN
CONSORTIUM: A DISTINCTIVE APPROACH TO
SOIL DECONTAMINATION
Soil contamination
method of microbial
degradation by bacteria and
fungi
consortium was conducted using microbial isolates
collected from different contaminated sites. the
species was isolated from the consortium of three
soils contaminated with heavy crude
The use of microbial consortia is effective in cases
where there is a lack of sufficient microorganisms
for degradation or there is a high toxicity of
contaminants
results it has been found that a consortium of
microbes is more effective than individual microbial
isolates microbial degradation process is quite
economical and does not cause ecological damage
Conclusion of the article obrva that applying several
microorganisms in combination is a universally efficient
system to achieve synergistic improved rates for the
degradation of PAH. PAH-contaminated soils can be
remedied using microorganisms (bacteria and fungi
individually or in combination
BIOLOGICAL TREATMENT OF TOTAL
PETROLEUM HYDROCARBONS
FROM AN OILY SLUDGE USING A
COMPOSTING APPROACH
pollution of selo and
water resources
co-composting
method
For this, the samples were taken from the
treatments on certain days (0, 7, 15, 30 and 60)
with the sterilized spatula. About 50 g of samples
were taken for each experiment and stored in
non-reactive plastic bags for TPH and C / N
analysis.
Results showed that most of the TPH of
the samples were extracted in the first 30
days. The maximum removal of TPHs
(65%) was obtained in the mud
the proportions of compost and the
control sample (p value <0.05)
indicated the adequate density of TPH
degrading bacteria in the treated
samples
selected for hydrocarbon
degrading bacteria and enhanced
hydrocarbon biodegradation in
slurry bioreactors
contamination of surface
and groundwater resources
microbiome-based biotechnologies to
treat hydrocarbon contamination and
enhanced biodegradation of
hydrocarbons in slurries
A sample of hydrocarbon-contaminated
weathered soils was obtained from a
production site in the Northwest Territories,
Canada. Mineralization experiments are
performed first Followed by an analysis of the
microbial community Gene expression, qPCR
and digital droplet PCR (ddPCR)
Result The analysis of the microbial community
indicated a significant enrichment of the phylum
of Actinobacteria and an increase in the absolute
abundance of the genus Dietzia that degrades
hydrocarbons, but a decrease in the total size of
the population with salinity.
Research findings. A degradation (up to 16 times greater) was
presented in a microbial community under saline conditions and
was associated with (up to 4.8 times) an increase in the absolute
abundance of a species that degrades hydrocarbons and up to 2.2
times the improvement in the mineralization rate constants. At
the same time, the population size decreased by up to 5.2 times in
the saline systems.
advanced treatment of
wastewater bioreactors
containing hydrocarbons
organic pollution
of the
environment
Aerobic
bioreactors
Samples and tests applied. A continuously
stirred tank bioreactor was applied to treat
hydrocarbon-rich wastewater from an oil
refinery using an acclimatized indigenous
microbial consortium.The performance of the
bioaugmented reactor was demonstrated by
reducing the chemical oxygen demand (COD)
rates to a 95% and the concentration of total
residual petroleum hydrocarbons (TPH) up to
97.5% (from 320 mg / L to 8 mg / L) Evaluated
the performance of a batch sequencing reactor
(SBR) with previously cultured activated sludge
microorganisms to treat synthetic
petrochemical wastewater containing mercury
and cadmium.
degradación microbiana de
hidrocarburos de petróleo
contamination of soils and
bodies of Water
Methodology used. We worked with tank
bottoms from the oil industry that have been
previously treated with water washing at 70 ° C
with biodegradable surfactant, which reduced
the hydrocarbon content
The initial characteristics of the tank bottom
make it a material for the use of biological
techniques in the elimination of hydrocarbons.
The residue from the tank bottom washing contained
bacteria in sufficient quantity for the use of the hydrocarbon
biodegradation techniques of the sample, this presented a
predominance of the genera Pseudomonas sp. and
Rhodococcus sp
Microbial degradation of petrochemical
residues-polycyclic aromatic
hydrocarbons
The problem of pollution is linked to economic, social
and political factors; that have led to a deterioration of
natural resources
Bioremediation is a technology that uses the
metabolic potential of microorganisms
(mainly bacteria, but also fungi and yeasts)
to transform organic pollutants into simpler
compounds
Among the techniques used for the remediation of
soils contaminated with PAH there are physical,
chemical, thermal and biological processes
The bioremediation of soils or areas of water
contaminated with crude oil, depend on the ability to
establish and maintain the appropriate conditions in the
microorganisms and thus favor the process, otherwise
the efficiency decreases
Bioremediation is a practice that is gaining importance
worldwide since the increase in industrial activity is
increasingly degrading natural ecosystems and the use of
known microorganisms for the treatment of potentially toxic
waste is already a common practice in developed countries. .