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Lac Operon
- involved in lactose breakdown in E.coli
- if no lactose, a repressor is bound to operator, blocking transcription
- RNAP's binding to DNA is controlled
- binds at promoter
- binds at promoter
- RNAP's binding to DNA is controlled
- if lactose present, it binds to the receptor and causes a shape change such so it no
longer binds to the operator and thus RNAP (Rna Polymerase) can transcribe the
genes
- if no lactose, a repressor is bound to operator, blocking transcription
- E. coli
- prefers glucose for energy
- prefers glucose for energy
- B-gal
- used to cleave glycosidic bond in lactose
- when cleaved makes glucose and galactose
- when cleaved makes glucose and galactose
- made by Lac z of the lac operon
- used to cleave glycosidic bond in lactose
- Lac Y is gene for creating B-galactosidepermease
- plac is sometimes called the promoter
- Laci is the gene that encodes for the repressor protein
- binds to the operator and represses function of this operon
- Pi is promoter of Laci
- always made and binding to the operator
- always made and binding to the operator
- blocks RNA polymerase
- Laci is bound to the DNA when lactose is not present
- when lactose is present it binds to the repressor which then falls off the repressor
- when lactose is present it binds to the repressor which then falls off the repressor
- binds to the operator and represses function of this operon
- Lac A is gene for transacetylase
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