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607393
Prokaryotic Diversity and Classification
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biosci 204 (lecture 3) Mapa Mental sobre Prokaryotic Diversity and Classification, criado por nfnabilah_14 em 06-03-2014.
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biosci 204
lecture 3
Mapa Mental por
nfnabilah_14
, atualizado more than 1 year ago
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Criado por
nfnabilah_14
quase 11 anos atrás
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Prokaryotic Diversity and Classification
What is prokaryote?
Archea
similar shape/size
multiply by binary fission
may have flagella
no peptidoglycan in cell wall
many are extremophiles
Hyperthermophile (over 100 degree celcius)
Pyrobolus
Halophiles (can grow in high salt medium)
Halobacterium *eventho it's actually not a bacterium*
Acidophiles (can grow in acid condition, best at pH 2)
Thermoplasma
Bacteria
Classifying prokaryotes
Morphological classification
Shape
coccus (round shape)
rod
spirillum (spiral shape)
spirochete (pasta-like)
budding & appendaged bacteria
filamentous bacteria
Arrangement
Cell size & significance of smallness
0.2 micrometres - 700 nanometres diameter
upper size limit
due to surface area to volume ratio for absoprtion of gases & nutrients
lower size limit
due to space needed to house essential proteins, nucleic acids etc
Biochemical classification
by using API kit (however it takes a long time to show the result)
Phylogenetics classification
Bacterial 16s rRNA genes
use to identify bacteria
highly variable among species
most of the bacteria have it!
Phylogenetic Overview of Bacteria
Isolate /amplify 16s RNA genes
sequence DNA and align sequences
construct the phylogenetic tree
Identifying and classifying microbial species
Binomial system of nomenclature
genus name
species name
Strain
Species
Basic concept of species on eukaryotes
Concept of species on microbes
Microbial classification
Name my own bacteria by writing a paper to International Journal of Systematic and Evolutionary Microbiology
The paper is published by them
I must deposit my organism in at least two international culture collections
ATCC (USA)
DSMZ (Germany)
JCM (Japan)
Anexos de mídia
lec_4.PNG (image/PNG)
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