Microscope

compound light microscope

dissecting (stereoscopic) microscope

Normal Procedure to prepare a specimen for most staining protocols

Problems with this procedure and why

Pros to Negative Staining

Negative Staining characteristics

The "why" behind the cells appearing clear when stained

Two different methods to prepare negative stains

Most microorganisms appear ______ when viewed through a ___________ _______ microscope.

Most common method method of allowing visualization of microorganisms under a microscope is to ...

Staining _________ to the microorganisms so that various structures can be observed

How to prepare a bacterial smear

Important facts about preparing the bacterial smear

Why is only a small amount of water needed?

When mixing the bacteria with the water to create the smear...

Once the smear has air-dried, the next step is to

Heat-fix