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Quiz sobre MCB Midterm Summary, criado por Chau Tran em 03-12-2019.

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MCB Midterm Summary

Questão 1 de 28

1

A/an _____________ vector quantifies the expression of a gene to measure the activity of regulatory elements.

Selecione uma das seguintes:

  • Reporter

  • Expression

  • Cloning

Explicação

Questão 2 de 28

1

An expression vector is used to _________.

Selecione uma das seguintes:

  • Study the effect of overexpression of a gene on cell functions

  • Measure activities of regulatory sequences

  • Quantify the expression of a gene downstream of the regulatory sequence of interest

  • Amplify and isolate the product of a gene

Explicação

Questão 3 de 28

1

Which of the following is NOT an essential component of a plasmid?

Selecione uma das seguintes:

  • Ori

  • Antibiotic resistance gene

  • Multiple cloning site

  • lac operon

Explicação

Questão 4 de 28

1

What would not be found in a eukaryotic expression vector?

Selecione uma ou mais das seguintes:

  • Bacterial Ori

  • Ampicillin selectable marker

  • Eukaryotic promoter

  • MCS

  • PolyA signal

  • Neomycin - eukaryotic selectable marker

  • Transfection factor

  • luciferase gene

Explicação

Questão 5 de 28

1

The expression of the _______ gene is quantified to study the activity of a regulatory sequence. In which type of vector would you find this gene?

Selecione uma das seguintes:

  • luciferase - expression vector

  • luciferase - reporter vector

  • lacZ - expression vector

  • lacZ - reporter vector

Explicação

Questão 6 de 28

1

Which is NOT involved in the construction of cDNA libraries?

Selecione uma das seguintes:

  • reverse transcriptase

  • oligo dT primer

  • ribonuclease H

  • DNA polymerase

  • DNA ligase

Explicação

Questão 7 de 28

1

What is the role of ribonuclease H in the synthesis of cDNA?

Selecione uma das seguintes:

  • Degrade the mRNA template

  • Synthesize the mRNA template

  • LIgate the 2 strands of newly-synthesized DNA

  • Synthesize the first strand of cDNA from mRNA template

Explicação

Questão 8 de 28

1

Which type of vector is pBluescript?

Selecione uma das seguintes:

  • Expression because it tests for the expression of lacZ gene

  • Cloning because it is used to isolate DNA sequences with a built-in mechanism to select for recombinant plasmids

  • Reporter because it quantifies lacZ expression

Explicação

Questão 9 de 28

1

Which outcome indicates that a colony on the pBluescript agar contains the recombinant plasmid?

Selecione uma das seguintes:

  • Colony is blue

  • Colony is white

  • There is a clearing zone around the colony

Explicação

Questão 10 de 28

1

Which is NOT true about enhancers sequences?

Selecione uma das seguintes:

  • Always located upstream of a gene

  • Function in both 5'-3' and 3'-5' directions

  • Influence expression of genes that are physically distant from the enhancer

  • Function is not affected if location is moved

Explicação

Questão 11 de 28

1

Which of the following are DNA binding motifs?

Selecione uma ou mais das seguintes:

  • Helix-turn-helix

  • Zinc finger

  • Leucine zipper

  • Helix-loop-helix

Explicação

Questão 12 de 28

1

_______ hormones bind hormone response elements (HRE) in the nucleus to upregulate target genes.

Selecione uma das seguintes:

  • Steroid

  • Peptide

Explicação

Questão 13 de 28

1

Epigenetics (changes in chromatin structure) is heritable and irreversible.

Selecione uma das opções:

  • VERDADEIRO
  • FALSO

Explicação

Questão 14 de 28

1

Position-effect variegation is the change in gene expression caused by the translocation of genes between heterochromatin and euchromatin regions.

Selecione uma das opções:

  • VERDADEIRO
  • FALSO

Explicação

Questão 15 de 28

1

Which does NOT result from histone acetyltransferases (HATs) activity?

Selecione uma das seguintes:

  • Loosening of DNA packaging

  • Decreased gene expression

  • Easier transcriptional factor binding

  • More open chromatin

Explicação

Questão 16 de 28

1

Which would lead to a decrease in gene expression?

Selecione uma ou mais das seguintes:

  • HAT

  • HDAC

  • DNA methyltransferases

  • SWI/SNF

Explicação

Questão 17 de 28

1

What is the difference in dosage compensation of Drosophila and C. elegans?

Selecione uma das seguintes:

  • Drosophila: hyperactivation of male X; C. elegans: hypoactivations of both female X'es

  • Drosophila: hypoactivations of both female X'es; C. elegans: hyperactivation of male X

  • Drosophila: inactivation of a female X; C. elegans: hyperactivation of male X

  • Drosophila: hyperactivation of male X; C. elegans: inactivation of a female X

Explicação

Questão 18 de 28

1

Imprinted gene is the inheritance of parental acetylation pattern.

Selecione uma das opções:

  • VERDADEIRO
  • FALSO

Explicação

Questão 19 de 28

1

Which does NOT result in increased mRNA stability?

Selecione uma das seguintes:

  • elF4E

  • PABPI

  • Deadenylase

  • Long poly-A tail

Explicação

Questão 20 de 28

1

______ removes the hairpin loop of the Pre-miRNA to form ds siRNA.

Selecione uma das seguintes:

  • Drosha

  • Dicer

  • Exp5

  • Ago2

Explicação

Questão 21 de 28

1

G1/S checkpoint is controlled by .
DNA damage checkpoint is controlled by .

Arraste e solte para completar o texto.

    Rb
    p53
    p21
    Ras

Explicação

Questão 22 de 28

1

Which is NOT correct regarding cell cycle control?

Selecione uma das seguintes:

  • Phosphorylation of Rb leads to growth factor independent G1/S progression.

  • A loss-of-function mutation in the p53 gene leads to a rapid accumulation of pro-cancer mutations.

  • Cyclin D is an inhibitor of Rb, which in turns is an inhibitor of E2F.

  • p53 upregulates BAX which activates the extrinsic apoptotic pathway.

Explicação

Questão 23 de 28

1

The p53 gene is mutated in >50% cancers.

Selecione uma das opções:

  • VERDADEIRO
  • FALSO

Explicação

Questão 24 de 28

1

Which gene is the most closely-associated with chronic myelogenous leukemia?

Selecione uma das seguintes:

  • bcr-abl

  • c-myc

  • Ras

  • HER2

Explicação

Questão 25 de 28

1

In Burkitt's lymphoma, ______ gene is translocated to a region controlled by a strong enhancer.

Selecione uma das seguintes:

  • c-myc

  • bcr-abl

  • HER2

  • Ras

Explicação

Questão 26 de 28

1

Which does NOT result in growth factor independent G1/S transition?

Selecione uma das seguintes:

  • Hyperphosphorylated Rb

  • Loss-of-function mutation of Rb

  • Mutation in E2F which prevents Rb binding

  • Low levels of cyclin D leading up to DNA duplication

Explicação

Questão 27 de 28

1

Which of the following cellular properties is the LEAST likely to cause cancer?

Selecione uma das seguintes:

  • Mutations in signalling proteins and receptors

  • Insensitive to anti-growth signals

  • Overexpression of pro-apoptotic proteins

  • Overexpression of telomerase

  • Upregulation of VEGF

  • Ability to metastasize and invade other tissues

Explicação

Questão 28 de 28

1

hyperactivation results from a point mutation to Valine and GTP-independent activity.
fusion protein is closely associated with chronic myelogenous leukaemia.
is an epidermal growth factor receptor, whose oncogenic properties arises from gene amplification.
In Burkitt's lymphoma, gene is translocated to a region controlled by a strong enhancer. It is also amplified in childhood neuroblastomas.

Arraste e solte para completar o texto.

    Ras
    bcr-abl
    HER2
    c-myc

Explicação

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