MEDT 2500: Chapter 1 Lesson Objectives

Describe the role of the innate and acquired immunity in the overall process of immunity.

Describe the role of:
-Phagocytes, and what cell lines they are

Describe the role of:
-Lymphocytes

Describe the role of:
-Cytokines

Describe the role of:
-Complement

List the general properties of antigens and their importance in immunohematology relative to the location on red cells, white cells, and platelets.

Identify the components of an immunoglobulin.

Compare and contrast IgM and IgG molecules.

Describe the major events in the primary and secondary immune response and the major differences between the two responses.

List the major forces that influence the binding of antigen and antibody.

Discuss the potential immunological consequences of transfusion and pregnancy.

Define the two stages (steps) of an agglutination reaction and the factors that influence these reactions in vitro.

Describe the different grading associated with the interpretation of agglutination reaction.

Describe the significance of hemolysis in blood banking.

Describe the basic principles of the antiglobulin testing.

List the indications for the use of direct and indirect antiglobulin testing.

List the functions of the different potentiators:
-LISS

List the functions of the different potentiators:
-Albumin

List the functions of the different potentiators:
-PEG

List the functions of the different potentiators:
Proteolytic enzymes

Antigen

Immunogen

Epitope

Antigenic determinants

Specificity

Chemical mediators

Vasodilation

Edema

Neutrophil

Acquired immunity

Monocytes

PMN's

T lymph

B lymph

Cellular immunity

Humoral immunity

Stem cell

Specificity

Antibody

Antigen

Determinant

Epitope

Plasma cell

Clone

MHC

Refractoriness

MAC

Hemolysis

Classical pathway

Alternative pathway

Anaphylatoxins

Vasoactive amines

Opsonin

Receptors

Immunogens

Carbohydrates

Lipids

Glycoprotien

Glycolipids

Allo-antibody

Ig

Heavy chains

Light chains

Kappa chains

Lambda chains

Variable region

Constant regions

Idiotype

Hinge region

Valence = dosage

Agglutination

Half life

Primary immune response

Secondary immune response

Anamnestic response

In vivo

Antibody screen test (IAT)

Multiparous

RES

In vitro

Sensitization

Lattice formation

Serum to cell ratio

IS = immediate spin

Zeta potential

Zone of equivalece

Prozone

Supernatant

Neutralization

DAT

IAT

Autoimmune hemolytic anemia

HDN = hemolytic disease of the newborn

Reaction phase

False negative reaction

False positive reaction

Potentiators

Enhancement media

Proteolytic enzymes