Constituative Chaperones

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Which of the following is not a role of the DNAK/Hsp70 superfamily?

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Hsp70s (unlike DNAK's) do not require chaperones

1

What is the role of DNAJ/Hsp40 when combined with DNAK/Hsp70

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DNAK/Hsp70 need NEF's in order to function

1

Where is the Hsp70/DNAK binding domain for ATP?

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Name the subdomains of the ATPase found in DNAK/Hsp70

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What increases the ATPase activity of the ATP binding domain?

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The core substrate binding domain has an affinity for what?

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The C-terminus of DNAK/Hsp 70 acts as a 'lid' for the protein

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When ATP is hydrolysed in DNAK/Hsp70, where does the C-terminus bind to?

1

Without the assistance of DNAJ/Hsp40, the ATPase in DNAK/Hsp70 works too slowly for the C-terminus to trap the bound substrate

1

Hsp70/DNAK's preferred binding pattern is 15 aa long

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What are the 'Flanking' residues (1-4, 9-13) of Hsp70's preferred binding site made of?

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The preferred residue for the -Core- of Hsp70's preferred binding site is Valine

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What other residues can make up the 'core' of the preferred binding site/

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How does this 'Preferred Binding Patter' for Hsp70 help direct the protein?

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What is the role of the J domain in a Hsp40?

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The role of the Gly/Phe rich linker does what?

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The Zinc 'Finger' is for substrate binding

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Which amino acids make up the HPD domain?

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The Znc finger of DNAJ/Hsp40 can interact with the nascent polypeptide and bring it to the DNAK/Hsp70

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What is the sequence of the Zinc 'finger'?

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The C-terminus of Hsp40 is for tetramerisation

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What effect does the GEF factor binding have on the Hsp70/Hsp40 complex?

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When the substrate & Hsp40 bind to Hsp70, the GEF factor also binds

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How is the nascent polypeptide transferred from the DNAJ site to the DNK site?

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In which of the following is Prefoldin found?

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Prefoldin can act as a substitute for Hsp70 if the organsim doesn't have the latter

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Prefoldin is ATP dependent

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Prefolding is a...

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What is the role of Preofoldin in Archea?

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In Eukaryotes, prefoldin might be specific for the folding of what?

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In archea there are 6 Prefoldin genes (Grim1-6) in Eukaryotes there are 2 (Gimalpha and Gimbeta)

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Which of the following describe Prefoldin

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There are 3.5 amino acid residues per helical turn of the coiled coil helices in Prefoldin (not 3.6)

1

Structural Basis for Biological Function (Protein Folding) Quiz sobre Constituative Chaperones, criado por gina_evans0312 em 20-12-2013.

Constituative Chaperones

Constituative Chaperones

Which of the following is not a role of the DNAK/Hsp70 superfamily?

Hsp70s (unlike DNAK's) do not require chaperones

What is the role of DNAJ/Hsp40 when combined with DNAK/Hsp70

DNAK/Hsp70 need NEF's in order to function

Where is the Hsp70/DNAK binding domain for ATP?

Name the subdomains of the ATPase found in DNAK/Hsp70

What increases the ATPase activity of the ATP binding domain?

The core substrate binding domain has an affinity for what?

The C-terminus of DNAK/Hsp 70 acts as a 'lid' for the protein

When ATP is hydrolysed in DNAK/Hsp70, where does the C-terminus bind to?

Without the assistance of DNAJ/Hsp40, the ATPase in DNAK/Hsp70 works too slowly for the C-terminus to trap the bound substrate

Hsp70/DNAK's preferred binding pattern is 15 aa long

What are the 'Flanking' residues (1-4, 9-13) of Hsp70's preferred binding site made of?

The preferred residue for the -Core- of Hsp70's preferred binding site is Valine

What other residues can make up the 'core' of the preferred binding site/

How does this 'Preferred Binding Patter' for Hsp70 help direct the protein?

What is the role of the J domain in a Hsp40?

The role of the Gly/Phe rich linker does what?

The Zinc 'Finger' is for substrate binding