recombinant protiens

costs associated with bringing recombinant protein to market

what influences the choice of organism?

plasmids can carry up to:

cosmids carry up to

general organism issues (recombinant protein production)

advantages of e coli for recombinant protein production

disadvantages of e coli for recombinant protein production

somatostatin (first reombinant protein made in e coli- anti growth hormone)

recombinant insulin (2 ways)

if your protein is active as a single unprocessed polypeptide...

If the activity of your protein is based on it’s complexity ....

7 post translational modifications of proteins

Pros of yeast and fungi

cons of yeast and fungi

pros of insect cells

cons of insect cells

plant recombinants

mammalian cells for recombs

glycosylation problems in higher eukaryotes

Gal alpha 1-3 Gal

rationale for recombinant protein production

are recombinants really safer?

all recombinants must be tested before human use

what are the in vivo animal tests looking at?

requirements for recombinant protien production

Waht do we need to make a recombinant protien?

recombinant protiens

where may recombinant protiens be produced?