Microbiology Lab Practical

Questão 1 de 127

1

What method of staining was used to create this slide?

Selecione uma das seguintes:

Acid-fast Stain
Endospore Stain
Gram Stain
Negative Stain

Explicação

Questão 2 de 127

1

What kind of staining process was used to make this slide?

Selecione uma das seguintes:

Negative Stain
Endospore Stain
Gram Stain
Acid-fast Stain

Explicação

Questão 3 de 127

1

What kind of microbes are present on this slide?

Selecione uma das seguintes:

Gram (-)
Endospores
Nocardia
Mycobacterium

Explicação

Questão 4 de 127

1

Where should your backpacks and purses be stored during the lab period?

Selecione uma das seguintes:

On the shelf under the lab bench.
On the floor next to your seat.
On the lab bench.

Explicação

Questão 5 de 127

1

What items should you wear to protect yourself in the lab.

Selecione uma ou mais das seguintes:

Lab coat
Safety Glasses
Gloves
Closed toe shoes
Hair net
Shoe covers

Explicação

Questão 6 de 127

1

When do you disinfect your lab table and where do you discard the used paper towels?

Selecione uma ou mais das seguintes:

Disinfect on the way into the lab.
Disinfect on the way out of the lab.
Paper towels go in the regular trash.
Paper towels go in the autoclave trash.
Disinfect between each step of the lab process.

Explicação

Questão 7 de 127

1

You are dying of thirst (hunger, for smoke, chapped lips, bad breath) during the lab period. What do you do to alleviate your problem?

Selecione uma das seguintes:

Leave
Disinfect your lab bench, take care of your need and then disinfect your lab bench, again.
Go to the corner of the lab room and take care of your need.

Explicação

Questão 8 de 127

1

You notice your lab partner has caught the lab manual on fire. What should you do?

Selecione uma ou mais das seguintes:

Alert the professor
Get the fire extinguisher near the front door
Get the fire extinguisher from the back of the room
Get deionized water from the back of the room and pour it on the fire

Explicação

Questão 9 de 127

1

Your lab partner has managed to spill microorganisms all over the lab table. What should be done to clean up the spill?

Selecione uma ou mais das seguintes:

Alert the professor
Spray disinfectant on the spill
Spray disinfectant on a paper towel and place on the spill
Keep spraying disinfectant on the paper towel
Wait 10 minutes
Wait 15 minutes
Dispose in autoclave trash
Dispose in regular trash

Explicação

Questão 10 de 127

1

A student broke an empty glass test tube. What should be done to clean up the glass and where should the pieces of glass be disposed of?

Selecione uma ou mais das seguintes:

Clean with dust pan and broom next to the fridge.
Clean with dust pan and broom near the front door.
Dispose of in the broken glass disposal box.
Dispose of in the autoclave trash.
Dispose of in the regular trash.

Explicação

Questão 11 de 127

1

The instructor tripped on a backpack on the floor knocking her safety goggles off and splashing HCl in her eyes. What should she do?

Selecione uma ou mais das seguintes:

Use the eyewash located near the front door.
Use the eyewash located at the back of the room.
Use the eyewash next to the fridge.
Use the eyewash near back of the room.

Explicação

Questão 12 de 127

1

After surviving the lab period you remove your gloves by turning them inside out and then where do you toss them?

Selecione uma ou mais das seguintes:

In the autoclave trash
In the regular trash
In the used glove disposal bin

Explicação

Questão 13 de 127

1

Choose the two major types of microscopes that have been developed and the difference between them

Selecione uma ou mais das seguintes:

Compound and Electron.
Compound uses light and Electron uses electrons.
Compound and Bright Field.
Compound uses electrons and Bright Field uses light.
Compound uses light and Bright Field uses electrons.

Explicação

Questão 14 de 127

1

Which microscopes are compound microscopes?

Selecione uma ou mais das seguintes:

Phase/Contrast Microscope
Bright Field Microscope
Dark Field Microscope
Fluorescent Microscope
Scanning Microscope
Objective Microscope

Explicação

Questão 15 de 127

1

The ability of the microscope to enlarge an object.

Selecione uma das seguintes:

Magnification
Resolving power.
Refractive index.
Resolution.

Explicação

Questão 16 de 127

1

How clear your specimen will appear when viewed through the lenses.

Selecione uma das seguintes:

Resolution
Refractive index
Resolving power
Magnification

Explicação

Questão 17 de 127

1

How well you lens is able to bend light.

Selecione uma das seguintes:

Refractive index.
Resolution
Resolving power
Magnification

Explicação

Questão 18 de 127

1

What components influence the resolving power of a microscope?

Selecione uma ou mais das seguintes:

Numerical aperture
Wavelength of light
Condenser
Diopter ring

Explicação

Questão 19 de 127

1

Concentrates the light beam and directs light through the specimen.

Selecione uma das seguintes:

Condenser
Iris diaphragm
Diopter ring
Refractive index

Explicação

Questão 20 de 127

1

Regulates the intensity of the light entering the lens.

Selecione uma das seguintes:

Iris diaphragm
Condenser
Diopter ring
Rheostat

Explicação

Questão 21 de 127

1

Focuses the individual eye piece to your eye.

Selecione uma das seguintes:

Diopter ring
Rheostat
Diaphragm ring
Iris diaphragm

Explicação

Questão 22 de 127

1

Steps to achieve adequate lighting when the oil immersion objective is used to view a specimen.

Selecione uma ou mais das seguintes:

Condenser raised to the highest position
Iris and base diaphragm are open
Rheostat is turned up
Condenser is lowered to the lowest position

Explicação

Questão 23 de 127

1

Components that influence the resolving power of a microscope.

Selecione uma ou mais das seguintes:

Numerical aperture
Wavelength of light
Rheostat level
Refractive index

Explicação

Questão 24 de 127

1

The isolation of a single species of microbe.

Selecione uma das seguintes:

Pure culture
Colony
Media
Inoculum
Turbdity

Explicação

Questão 25 de 127

1

Cells from a single asexual parent that are identical.

Selecione uma das seguintes:

Colony
Pure culture
Media
Inoculum
Turbidity

Explicação

Questão 26 de 127

1

Nutrient material suitable for the cultivation of microorganisms.

Selecione uma das seguintes:

Media
Pure culture
Colony
Turbdity
Inoculum

Explicação

Questão 27 de 127

1

Transfer of a pure culture from a slant culture to a new sterile broth. The sampling of a bacterial culture.

Selecione uma das seguintes:

Inoculum
Pure culture
Colony
Media
Turbidity

Explicação

Questão 28 de 127

1

Cloudiness in broth that indicates growth.

Selecione uma das seguintes:

Turbidity
Pure culture
Media
Colony
Inoculum

Explicação

Questão 29 de 127

1

Steps involved in labeling a test tube.

Selecione uma das seguintes:

Label a piece of labeling tape with your name and the name of the bacteria and place on test tube.
Use a wax pencil and label the test tube with your name and the name of the bacteria.
Use a marker and label the test tube with your name and the name of the bacteria.

Explicação

Questão 30 de 127

1

What are the steps for labeling a Petri plate?

Selecione uma das seguintes:

Label the bottom of the media side using a wax pencil or marker with your name and the organism you are transferring.
Label the lid using a wax pencil or marker with your name and the name of the organism you are transferring.
Label a piece of labeling tape with your name and the name of the organism you are transferring and place it on the lid of the Petri plate.

Explicação

Questão 31 de 127

1

What are the steps for incubating a Petri plate?

Selecione uma das seguintes:

Sterilize inoculating loop in Bacticinerator. Lightly drag the tip of the loop from side to side or back and forth in order to spread the inoculum. Replace lid. Incubate lid side up.
Sterilize inoculating loop in Bacticinerator. Lightly drag the tip of the loop from side to side or back and forth in order to spread the inoculum. Replace lid. Incubate lid side down.

Explicação

Questão 32 de 127

1

What is meant by the statement that microorganisms are ubiquitous?

Selecione uma das seguintes:

Microorganisms are everywhere
Microorganisms are hard to identify
Microorganisms are difficult to see without a microscope
Microorganisms come in many forms

Explicação

Questão 33 de 127

1

Handling of bacteria in a manner so as to prevent contaminating laboratory media and to prevent infecting ourselves and our classmates.

Selecione uma das seguintes:

Aseptic technique
Sterile transfer
Inoculation technique
Ubiquitous

Explicação

Questão 34 de 127

1

Why are "basic" dyes used in the Gram stain?

Selecione uma das seguintes:

Bacteria have an overall negative charge so stain with positive (basic) dyes. Opposites attract.
Bacteria have an overall positive charge so stain with positive (basic) dyes. Likes attract likes.

Explicação

Questão 35 de 127

1

State two differences between a simple stain and a differential stain.

Selecione uma das seguintes:

Simple stain uses one dye; all bacteria are the same color; shows size, shape and arrangement. Differential stain uses two dyes; different bacteria are stained contrasting colors; shows size, shape, arrangement and something else.
Differential stain uses one dye; all bacteria are the same color; shows size, shape and arrangement. Simple stain uses two dyes; different bacteria are stained contrasting colors for simple identification; shows size, shape, arrangement and something else.

Explicação

Questão 36 de 127

1

A) Has a high lipid content and thin layer of tecoic acid underneath and stains pink.
B) Has a low lipid content and thick layer containing tecoic acids and stains purple.

Selecione uma das seguintes:

A) Gram negative bacteria
B) Gram positive bacteria
A) Gram positive bacteria
B) Gram negative bacteria
A) Gram stain
B) Acid-fast stain
A) Acid-fast stain
B) Gram stain

Explicação

Questão 37 de 127

1

What us the importance of using only a pinpoint of inoculum for the smear preparation?

Selecione uma ou mais das seguintes:

Insure proper staining
Insure proper decolorizing
The slide is small
Allows you to prepare only one or two bacteria.

Explicação

Questão 38 de 127

1

What is the importance of allowing the smear to air dry?

Selecione uma ou mais das seguintes:

Insure the cells stay intact and stain properly.
Insure the cells are dead and stain properly
Insure the cells wont move around during staining

Explicação

Questão 39 de 127

1

What is the importance of decolorizing smears one at a time?

Selecione uma das seguintes:

It is the most critical step and there is no hard and fast rule on how long to decolorize.
It is the most critical step and don't want to drop the slides.
It is the most critical step and need to time it exactly.

Explicação

Questão 40 de 127

1

List the gram stain reagents in order and state their function.

Selecione uma das seguintes:

1) Crystal Violet - Primary stain
2) Iodine - Mordant, combines with the crystal violet in the cell.
3) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer
4) Safranin - Counterstain or Secondary stain
1) Crystal Violet - Primary stain
2) Safranin - Counterstain or Secondary stain
3) Iodine - Mordant, combines with the crystal violet in the cell.
4) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer
1) Crystal Violet - Primary stain
2) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer
3) Iodine - Mordant, combines with the crystal violet in the cell.
4) Safranin - Counterstain or Secondary stain
1) Crystal Violet - Primary Stain
2) Iodine - Mordant, combines with the crystal violet in the cell
3) Safranin - Counterstain or Secondary stain
4) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer

Explicação

Questão 41 de 127

1

Why do gram negative cells lose the primary dye during decolorization?

Selecione uma das seguintes:

The high lipid content in gram negative cell walls causes them to dissolve in the ethanol allowing the crystal violet dye to leak out.
The low lipid content in gram negative cell walls causes them to dissolve in the ethanol allowing the crystal violet dye to leak out.

Explicação

Questão 42 de 127

1

Why is the Acid-fast stain considered a "differential stain"?

Selecione uma ou mais das seguintes:

It distinguishes between two groups of organisms based on the lipid content in their cell walls.
It uses two positively charged dyes.
It uses one positively charged dye and one negatively charged dye.
It tells size, shape, arrangement and something else.
It tells size, shape and arrangement.

Explicação

Questão 43 de 127

1

What is the difference between the decolorizer used in the Gram stain and the decolorizer used in the Acid-fast stain?

Selecione uma das seguintes:

Acid fast uses a solution of 3% HCl and 95% ethanol. Gram stain uses just ethanol.
Acid fast uses a solution of 95% HCl and 3% ethanol. Gram stain uses just ethanol.

Explicação

Questão 44 de 127

1

Lipids in the cell's waxy wall prevent the acid-alcohol from decolorizing the cell.

Selecione uma das seguintes:

Acid-fast
Gram
Negative
Endospore

Explicação

Questão 45 de 127

1

What is the difference between the Ziehl-Neelsen stain and the Kinyoun stain?

Selecione uma das seguintes:

Ziehl-Neelsen uses steam to penetrate the mycolic acid in cell walls.
Kinyoun uses concentrated dye, hard heat fixing and longer exposure time.
Kinyoun uses steam to penetrate the mycolic acid in cell walls.
Ziehl-Neelsen uses concentrated dye, hard heat fixing and longer exposure time.
Ziehl-Neelsen uses hard heat fixing to penetrate the mycolic acid in cell walls.
Kinyoun uses concentrated dye, steam and longer exposure time.
Kinyoun uses hard heat fixing to penetrate the mycolic acid in cell walls.
Ziehl-Neelsen uses concentrated dye, steam and longer exposure time.

Explicação

Questão 46 de 127

1

Which are acid-fast organisms and what disease do they cause?

Selecione uma ou mais das seguintes:

Mycobacterium tuberculosis
Disease: Tuberculosis
Mycobacterium leprae
Disease: Leprosy or Hansen's disease
Nocardia asteroides
Disease: Nocardiosis
Cryptosporidium
Disease: Cryptosporidiosis
Mycosporidium
Disease: Mycardiosis
Nocardia tuberculosis
Disease: Tuberculosis

Explicação

Questão 47 de 127

1

What are the reactants in the acid-fast stain, in order?

Selecione uma das seguintes:

1) Carbolfuchsin
2) Acid-alcohol
3) Loeffler's methylene blue
1) Loeffler's methylene blue
2) Acid-alcohol
3) Carbolfichsin
1) Carbolfuschsin
2) Iodine
3) Acid-alcohol
4) Loeffler's methylene blue
1) Loeffler's methylene blue
2) Iodine
3) Acid-alcohol
4) Carbolfuchsin

Explicação

Questão 48 de 127

1

Two methods used to force the dye into an endospore.

Selecione uma ou mais das seguintes:

Steam for 3-5 minutes
Hard heat fixing then letting the dye remain on the slide for an extended period of time.
Hard heat fixing
Steam then letting the dye remain on teh slide for an extended period of time.

Explicação

Questão 49 de 127

1

What dyes are used in the Schaffer-Fulton spore stain?

Selecione uma ou mais das seguintes:

Malachite green
Gram's safranin
Iodine
Loeffler's methylene blue

Explicação

Questão 50 de 127

1

In the "Christmas tree" stain, the endospores are colored _ and the vegetative cells are colored ____.

Selecione uma ou mais das seguintes:

Green
Red
Red
Green

Explicação

Questão 51 de 127

1

Metabolically active, replicating cell.

Selecione uma ou mais das seguintes:

Vegetative cell
Endospore
Germinated cell

Explicação

Questão 52 de 127

1

The formation of endospores within a vegetative cell

Selecione uma das seguintes:

Sporogenesis
Decolorization
Germination

Explicação

Questão 53 de 127

1

When an endospore reverts to a vegetative state.

Selecione uma das seguintes:

Germination
Sporogenesis
Growth

Explicação

Questão 54 de 127

1

Why are endospores considered "the most resistant life form known"?

Selecione uma ou mais das seguintes:

Can remain dormant indefinitely
Resistant to antibiotics and most disinfectants
Resistant to radiation
Resistant to boiling
Resistant to drying
Impermeability of spore coat

Explicação

Questão 55 de 127

1

What are the medically important endospore-producing bacteria and their associated disease?

Selecione uma ou mais das seguintes:

Bacillus anthracis
Disease: anthrax
Clostridium botulinum
Disease: Botulism
Clostridium difficile
Disease: Toxic enterocolotis
Clostridium perfringens
Disease: Gas gangrene, food poisoning
Clostridium tetani
Disease: Tetnus, lockjaw
Clostridium anthracis
Disease: Anthrax

Explicação

Questão 56 de 127

1

Which medically important genera of bacteria produce endospores?

Selecione uma ou mais das seguintes:

Bacillus
Clostridium
Streptococcus
Staphylococcus

Explicação

Questão 57 de 127

1

A positive stain _ and a negative stain __.

Selecione uma das seguintes:

Stains what you want to see
Stains everything but the structure you want to see
Stains everything but the structure you want to see
Stains what you want to see
Stains gram (+) bacteria
Stains gram (-) bacteria
Stains gram (-) bacteria
Stains gram (+) bacteria

Explicação

Questão 58 de 127

1

Why are Nigrosin and India ink commonly used negative stains?

Selecione uma das seguintes:

They are negatively charged dyes and are repelled by the negative contents of the bacterial cell.
They are positively charged dyes and are repelled by the positive contents of the bacterial cell.
They are positively charged dyes and are attracted by the negative contents of the bacterial cell.
They are negatively charged dyes and are attracted by the positive contents of the bacterial cell.

Explicação

Questão 59 de 127

1

What are the advantages of using a negative stain versus a positive stain?

Selecione uma ou mais das seguintes:

Able to see a capsule
Do not heat fix so can see them as they are
Can be used for things that don't stain.
Can see if it produces endospores
Can see if it is gram (-) or gram (+)

Explicação

Questão 60 de 127

1

Sticky substance that is tightly bound, highly organized and generally round or oval.

Selecione uma das seguintes:

Capsule
Slime layer
Endospore

Explicação

Questão 61 de 127

1

Sticky substance is loosely bound and somewhat irregularly shaped.

Selecione uma das seguintes:

Capsule
Slime layer
Endospore

Explicação

Questão 62 de 127

1

How does a capsule contribute to the virulence of a bacteria?

Selecione uma ou mais das seguintes:

Helps the bacteria avoid phagocytosis by white blood cells
Helps the bacteria attach to skin and mucous membranes
Is a source of nutrition for the bacteria when resources are scarce
Contains the pathogenic part of the bacteria

Explicação

Questão 63 de 127

1

What are the difference(s) between the smear preparation for a Gram stain and a capsule stain?

Selecione uma ou mais das seguintes:

A smear for a capsule stain is not heat fixed.
A spear from a capsule stain is not rinsed with deionized water it is rinsed with copper sulfate
A smear for a capsule stain uses steam
A smear for a Gram stain uses crystal violet and a capsule stain does not

Explicação

Questão 64 de 127

1

Using Anthony's stain, the background will be colored and the capsule will be colored _____.

Selecione uma das seguintes:

Dark blue
Light blue to white
Light blue to white
Dark blue

Explicação

Questão 65 de 127

1

What are the damaging by-products of oxygen during cellular respiration?

Selecione uma ou mais das seguintes:

Superoxides
Peroxides
Carbon dioxide
Carbon monoxide

Explicação

Questão 66 de 127

1

What enzymes neutralize the damaging effects of the by-products of oxygen during cellular respiration?

Selecione uma ou mais das seguintes:

Catalase
Peroxidase
Superoxide dismutase
Carbonase

Explicação

Questão 67 de 127

1

Uses oxygen as the final member of the ETC and has enzymes to break down the toxic by-products of oxygen.

Selecione uma das seguintes:

Aerobe
Anerobe

Explicação

Questão 68 de 127

1

Does not use oxygen as the final member of the ETC and lacks the enzymes to breakdown the toxic by-products of oxygen.

Selecione uma das seguintes:

Aerobe
Anaerobe

Explicação

Questão 69 de 127

1

How does a candle jar achieve an atmosphere to grow microaerophilic and capnophilic organisms?

Selecione uma das seguintes:

The flame burns using up the oxygen and producing CO2
The flame burns up the CO2 and produces oxygen.

Explicação

Questão 70 de 127

1

How does a Gas Pak jar achieve an anaerobic atmosphere?

Selecione uma das seguintes:

The pak contains chemicals that produce H+ and CO2. The H+ combines with the O2 in the jar to produce H2O.
The pak contains chemicals that produce CO2 and destroy O2.

Explicação

Questão 71 de 127

1

Which type of organism grows equally well in all types of oxygen concentrations?

Selecione uma das seguintes:

Facultative anaerobes
Microaerophiles
Capnophiles
Aerotolerant anaerobes

Explicação

Questão 72 de 127

1

How does thioglycolate broth grow anaerobic bacteria as well as aerobic bacteria?

Selecione uma ou mais das seguintes:

The agar prevents diffusion of O2
It contains a reducing agent, sodium thioglycollate, that combines with O2 to produce water
It contains a reducing agent, sodium thioglycollate, that combines with the agar to produce CO2 near the bottom
The agar prevents diffusion of CO2

Explicação

Questão 73 de 127

1

Organisms that require around 20% atmospheric oxygen.
(Check the name of the organism and the media on which it will grow.)

Selecione uma ou mais das seguintes:

Strict aerobe
Facultative anaerobe
Only at the top of the thioglycollate broth.
Candle jar
Gas pak
Capnophile

Explicação

Questão 74 de 127

1

Organisms that require between 5-15% atmospheric oxygen for growth
(Check the name of the organism and the media on which it will grow.)

Selecione uma ou mais das seguintes:

Microaerophile
Facultative anaerobe
Grows in candle jar
Grows at the point where the oxygen level is diminishing in the thio broth.
Grows in the GasPak

Explicação

Questão 75 de 127

1

Organisms that require between 5-15% atmospheric oxygen and increased (10%) carbon dioxide.
(Check the name of the organism and the media on which it will grow.)

Selecione uma ou mais das seguintes:

Capnophile
Microaerophile
Grows in candle jar
Grows in oxygen
Grows in a GasPak

Explicação

Questão 76 de 127

1

Organisms that will use a fermentative metabolic pathway in anaerobic conditions yet can convert to aerobic cellular respiration when atmospheric oxygen is present. They grow equally well in aerobic anaerobic environments.

Selecione uma ou mais das seguintes:

Facultative anaerobe
Microaerophile
Grows in oxygen
Grows in candle jar
Grows in GasPak
Grows throughout the entire thio broth slant

Explicação

Questão 77 de 127

1

Organism that can exist in the presence of oxygen, but does not use oxygen for metabolism.

Selecione uma ou mais das seguintes:

Aerotolerant anaerobe
Microaerophile
All over the thio broth slant but slightly less at the top
Grows in the GasPak
Capnophile

Explicação

Questão 78 de 127

1

Organisms that die in the presence of atmospheric oxygen because they lack the enzymes needed to breakdown the toxic by-products.

Selecione uma ou mais das seguintes:

Strict anaerobe
Grows in GasPak
Grows in candle jar
Grows toward the bottom of the thio broth
Grows toward the top of the thio broth

Explicação

Questão 79 de 127

1

Which gene allows jellyfish to glow in the dark?

Selecione uma das seguintes:

Green fluorescent protein
Fluorescent blue plasmid
GFP
FBP

Explicação

Questão 80 de 127

1

Bacteria cells able to take up the plasmid/DNA.

Selecione uma das seguintes:

Competent cells
Competent genes
Competent proteins

Explicação

Questão 81 de 127

1

What laboratory procedures are done to encourage cells to become competent?

Selecione uma ou mais das seguintes:

Mixing cells with calcium chloride during growth phase
Exposing cells to extreme temperature changes during the proceduree
Mixing cells with with heated calcium hydroxide
Freezing cells

Explicação

Questão 82 de 127

1

What is the relationship between a gene and a protein?

Selecione uma das seguintes:

A gene is a piece of DNA that provides the instructions for making a protein
A protein is a piece of DNA that provides the instructions for making a gene
DNA is a piece of a protein that provides the instructions for making a gene

Explicação

Questão 83 de 127

1

Chromosomal DNA _ and plasmids _______.

Selecione uma das seguintes:

Carries genes needed for the hereditary characteristics essential for bacterial growth and reproduction.
Small pieces of circular DNA that have genes for one or more traits that may be beneficial but are not essential for bacterial survival.
Small pieces of circular DNA that have genes for one or more traits that may be beneficial but are not essential for bacterial survival.
Carries genes needed for the hereditary characteristics essential for bacterial growth and reproduction.
Carries genes needed for the hereditary characteristics that may be beneficial but are not essential for bacterial growth and reproduction.
Small pieces of circular DNA that have genes for one or more traits that are essential for bacterial survival.

Explicação

Questão 84 de 127

1

Which genes are contained in the pGLO plasmid?

Selecione uma ou mais das seguintes:

GFP
BLA
Ara-C
Ara-B

Explicação

Questão 85 de 127

1

How is expression of the Green Fluorescent Protein gene regulated?

Selecione uma das seguintes:

The Ara-C gene controls expression of GFP via the sugar arabinose
The Ara-B gene controls expression of GFP via the sugar arabinose
The Ara-C gene controls expression of GFP via the sugar glucose

Explicação

Questão 86 de 127

1

Chemicals produced by microorganisms that in small quantities can inhibit the growth of other microorganisms

Selecione uma das seguintes:

Antibiotic
MIC
E-test

Explicação

Questão 87 de 127

1

Determines the lowest concentration of an antibiotic that is able to inhibit the growth of a test organism.

Selecione uma das seguintes:

Antibiotic
MIC
E-test

Explicação

Questão 88 de 127

1

A plastic coated strip that contains a gradient

Selecione uma das seguintes:

Antibiotic
MIC
E-test

Explicação

Questão 89 de 127

1

What are the disadvantages of using a broad spectrum antibiotic?

Selecione uma ou mais das seguintes:

Contributes to escalating drug resistance
Wipes out a person's normal flora resulting in superinfections
Only works on weak bacteria

Explicação

Questão 90 de 127

1

What does the antibiotic test result "sensitive" indicate about the test organism?

Selecione uma ou mais das seguintes:

An organism is susceptible to drug concentrations obtained in the body using normal therapeutic doses.
The antibiotic is likely to be effective against the pathogen
The antibiotic is not likely to work.

Explicação

Questão 91 de 127

1

What factors must be controlled in order to standardize the Kirby-Bauer test?

Selecione uma ou mais das seguintes:

Stability of the antibiotic
Rate of diffusion of the antibiotic
pH of the culture medium
Depth of the culture medium
Inoculum density
Incubation time
Incubation temperature
Concentration of antibiotic

Explicação

Questão 92 de 127

1

A?

Selecione uma das seguintes:

Arm
Rheostat
Power switch
Coarse focus knob

Explicação

Questão 93 de 127

1

B?

Selecione uma das seguintes:

Rheostat
Power switch
Coarse focus knob
Arm

Explicação

Questão 94 de 127

1

C?

Selecione uma das seguintes:

Rheostat
Power switch
Coarse focus knob
Fine focus knob

Explicação

Questão 95 de 127

1

D?

Selecione uma das seguintes:

Coarse focus knob
Fine focus knob
RHeostat
Power switch

Explicação

Questão 96 de 127

1

E?

Selecione uma das seguintes:

Fine focus knob
Coarse focus knob
Rheostat
Power switch

Explicação

Questão 97 de 127

1

A?

Selecione uma das seguintes:

Ocular
Diopter
Diaphragm ring
Fine focus knob

Explicação

Questão 98 de 127

1

B?

Selecione uma das seguintes:

Mechanical stage
Base
Slide stage
Ocular stage

Explicação

Questão 99 de 127

1

C?

Selecione uma das seguintes:

Fine focus knob
Coarse focus knob

Explicação

Questão 100 de 127

1

D?

Selecione uma das seguintes:

Base
Mechanical stage
Mechanical base

Explicação

Questão 101 de 127

1

A
B
C
D
E

Selecione uma das seguintes:

A - Objective lens
B - Iris diaphragm lever
C - Condenser centering screw
D - Base diaphragm
E - Mechanical stage knob
A - Ocular lens
B - Iris diaphragm lever
C - Condenser centering screw
D - Base diaphragm
E - Mechanical stage knob
A - Ocular lens
B - Condenser centering screw
C - Iris diaphragm lever
D - Base diaphragm
E - Mechanical stage knob
A - Ocular lens
B - Iris diaphragm lever
C - Mechanical stage knob
D - Base diaphragm
E - Condenser centering screw

Explicação

Questão 102 de 127

1

A
B
C

Selecione uma das seguintes:

A - Iris diaphragm lever
B - Condenser centering screw
C - Base diaphragm
A - Condenser centering screw
B - Iris diaphragm lever
C - Base diaphragm
A - Iris diopter screw
B - Iris diaphragm lever
C - Base diaphragm
A - Iris diopter lever
B - Condenser centering screw
C - Base diaphragm

Explicação

Questão 103 de 127

1

A
B

Selecione uma das seguintes:

A - Revolving nose piece
B - Mechanical stage clip
A - Objective ring
B - Mechanical stage clip
A - Diopter ring
B - Mechanical stage clip

Explicação

Questão 104 de 127

1

A
B
C

Selecione uma das seguintes:

A - Condenser knob
B - Condenser
C - Base diaphragm ring
A - Condenser knob
B - Condenser ring
C - Base
A - Condenser ring
B - Condenser
C - Base

Explicação

Questão 105 de 127

1

In the thioglycollate test tube A, what kind of organism does the growth represent?

Selecione uma das seguintes:

Microaerophile
Obligate/Strict aerobe
Facultative anaerobe
Obigate/strict anaerbe

Explicação

Questão 106 de 127

1

In the thioglycollate test tube B, what kind of organism does the growth represent?

Selecione uma das seguintes:

Microaerophile
Obligate/Strict aerobe
Facultative anaerobe
Obligate anaerobe

Explicação

Questão 107 de 127

1

In the thioglycollate test tube C, what kind of organism does the growth represent?

Selecione uma das seguintes:

Microaerophile
Obligate/strict aerobe
Facultative anaerobe
Obligate/strict anaerobe

Explicação

Questão 108 de 127

1

In the thioglycollate test tube D, what kind of organism does the growth represent?

Selecione uma das seguintes:

Microaerophile
Obligate/strict aerobe
Facultative anaerobe
Obligate/strict anaerobe

Explicação

Questão 109 de 127

1

On a luria plate with Ampicillin, why is there no E. coli growth?

Selecione uma das seguintes:

Because E.coli are sensitive to Ampicillin
Because E. coli are resistant to Ampicillin
Because luria does not contain the nutrients necessary for growth.

Explicação

Questão 110 de 127

1

What protein allowed E. coli to grow on the plate with luria, Ampicillin and pGlo?

Selecione uma das seguintes:

Beta lactamase
BLA
GFP
Green fluorescent protein

Explicação

Questão 111 de 127

1

What gene allowed the E. coli to grow on the plate with luria, Ampicillin and pGlo?

Selecione uma das seguintes:

BLA
Beta lactamase
GFP
Green fluorescent protein

Explicação

Questão 112 de 127

1

What molecule allowed the E. coli to glow like a jellyfish?

Selecione uma das seguintes:

Arabinose
Beta lactamase
Ara-C
Green fluorescent protein

Explicação

Questão 113 de 127

1

What media is used for the antibiotic sensitivity test?

Selecione uma das seguintes:

Mueller hinton
Kirby Bauer
Luria

Explicação

Questão 114 de 127

1

What is the result of the beta lactamase test pictured?

Selecione uma das seguintes:

It is pink and therefore, the test is negative and the microbe is sensitive to penicillin
it is pink and therefore, the test is positive and the microbe is sensitive to penicillin
it is pink and therefore, the test is positive and the microbe is resistant to penicillin
It is pink and therefore, the test is negative and the microbe is resistant to penicillin

Explicação

Questão 115 de 127

1

Beta lactamase test

Selecione uma das seguintes:

It is yellow and therefore, the test is positive and the microbe is resistant to penicillin
It is yellow and therefore, the test is negative and the microbe is resistant to penicillin
It is yellow and therefore, the test is positive and the microbe is sensitive to penicillin
It is yellow and therefore, the test is negative and the microbe is sensitive to penicillin

Explicação

Questão 116 de 127

1

Why us thioglycollate a semi-solid agar?

Selecione uma das seguintes:

Has agar in it and contains a reducing agent that produces water
Has agar and a reducing agent that produces CO2

Explicação

Questão 117 de 127

1

Why are agar plates labeled on the bottom and inverted when incubated?

Selecione uma das seguintes:

Labeled on the bottom in case you lose the lid and inverted during incubation to prevent condensation from dripping onto the agar surface
Labeled on the bottom in case you lose the lid and inverted so you can read the label

Explicação

Questão 118 de 127

1

Why hold the test tube with your little finger while taking your bacterial sample?

Selecione uma das seguintes:

So you do not contaminate the cap.
So the cap doesn't roll away.
So you can stretch your fingers.

Explicação

Questão 119 de 127

1

Describe the proteins encoded in the pGLO plasmid.

Selecione uma ou mais das seguintes:

Green fluorescent protein
Beta lactamase
Ara-C represser protein
Beta green protein

Explicação

Questão 120 de 127

1

What is the principal of the beta lactamase test?

Selecione uma das seguintes:

Does the bacteria have the BLA gene and therefore produce beta lactamase
Does the bacteria have the BLA protein and therefore produce the beta lactamase gene

Explicação

Questão 121 de 127

1

Explain the regulation mechanism of the Green Fluorescent Protein gene expression.

Selecione uma das seguintes:

Arabinose has to move the Ara-C represser protein out of the way so GFP can be transcribed and translated.
GFP takes up the arabinose so Ara-C can be transcribed and translated.

Explicação

Questão 122 de 127

1

Define reducing agent

Selecione uma das seguintes:

Sodium thioglycollate is a reducing agent and it reduces by binding O2 and converting it to water
Sodium thioglycollate is a reducing agent that reduces by causing evaporation of the broth

Explicação

Questão 123 de 127

1

What are the criteria used to describe a bacterial colony>

Selecione uma ou mais das seguintes:

Surface
Elevation
Whole colony
Edge
Color

Explicação

Questão 124 de 127

1

Locations of items in the lab

Selecione uma ou mais das seguintes:

Near the front door: Fire extinguisher, Eye wash, Shower, Gloves
Back of room: Fume hood
Lab bench: Disinfectant, Autoclave bags, Paper towels
Near Sinks: Bleach, Dyes used for stains, Paper towels (above)
Fume hood: Broken glass disposal box (underneath), Autoclave bags
Front podium: Phone
Back: Test tube racks, MSDS notebooks (back corner),
Next to fridge: Dustpan and broom

Explicação

Questão 125 de 127

1

Which items are disposed of in the autoclave trash?

Selecione uma ou mais das seguintes:

Used glass test tubes
Used glass slides
Broken glass
Used latex gloves
Paper towels used for bench disinfection
Contaminated Petri plates

	Criado por Elle Ashe quase 11 anos atrás

MCC Microbiology Lab Practical 2

Microbiology Lab Practical

Questão 1 de 127

What method of staining was used to create this slide?

Selecione uma das seguintes:

Explicação

Questão 2 de 127

What kind of staining process was used to make this slide?

Selecione uma das seguintes:

Explicação

Questão 3 de 127

What kind of microbes are present on this slide?

Selecione uma das seguintes:

Explicação

Questão 4 de 127

Where should your backpacks and purses be stored during the lab period?

Selecione uma das seguintes:

Explicação

Questão 5 de 127

What items should you wear to protect yourself in the lab.

Selecione uma ou mais das seguintes:

Explicação

Questão 6 de 127

When do you disinfect your lab table and where do you discard the used paper towels?

Selecione uma ou mais das seguintes:

Explicação

Questão 7 de 127

You are dying of thirst (hunger, for smoke, chapped lips, bad breath) during the lab period. What do you do to alleviate your problem?

Selecione uma das seguintes:

Explicação

Questão 8 de 127

You notice your lab partner has caught the lab manual on fire. What should you do?

Selecione uma ou mais das seguintes:

Explicação

Questão 9 de 127

Your lab partner has managed to spill microorganisms all over the lab table. What should be done to clean up the spill?

Selecione uma ou mais das seguintes:

Explicação

Questão 10 de 127

A student broke an empty glass test tube. What should be done to clean up the glass and where should the pieces of glass be disposed of?

Selecione uma ou mais das seguintes:

Explicação

Questão 11 de 127

The instructor tripped on a backpack on the floor knocking her safety goggles off and splashing HCl in her eyes. What should she do?

Selecione uma ou mais das seguintes:

Explicação

Questão 12 de 127

After surviving the lab period you remove your gloves by turning them inside out and then where do you toss them?

Selecione uma ou mais das seguintes:

Explicação

Questão 13 de 127

Choose the two major types of microscopes that have been developed and the difference between them

Selecione uma ou mais das seguintes:

Explicação

Questão 14 de 127

Which microscopes are compound microscopes?

Selecione uma ou mais das seguintes:

Explicação

Questão 15 de 127

The ability of the microscope to enlarge an object.

Selecione uma das seguintes:

Explicação

Questão 16 de 127

How clear your specimen will appear when viewed through the lenses.

Selecione uma das seguintes:

Explicação

Questão 17 de 127

How well you lens is able to bend light.

Selecione uma das seguintes:

Explicação

Questão 18 de 127

What components influence the resolving power of a microscope?

Selecione uma ou mais das seguintes:

Explicação

Questão 19 de 127

Concentrates the light beam and directs light through the specimen.

Selecione uma das seguintes:

Explicação

Questão 20 de 127

Regulates the intensity of the light entering the lens.

A) Has a high lipid content and thin layer of tecoic acid underneath and stains pink.
B) Has a low lipid content and thick layer containing tecoic acids and stains purple.