Let's Learn Agars

Indole:
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive Reaction: ❌, Negative Reaction: ❌
End Product: Indole

Methyl Red
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌
End Product: Mixed Acids

Voges-Proskauer
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌
End-Product: Acetoin

Citrate
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Postive: ❌, Negative: ❌
End Product: Sodium Carbonate

Decarboxylation
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌
End Product: Primary amine for amino acid

Deamination Phenylalanine or TDA
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌ (Hint: color of the slant)
End Product: Phenyl Pyruvic Acid

Gelatin
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌
End Product: Liquid or Solid

ONPG
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌
End Product: Ortho Nitrophenol

Nitrate
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌ after reagent, colorless after zinc, Negative: Colorless after reagents, Red after zinc
End Product: N/A

Lysine Iron Agar
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌ (and black is H2S)
End Product: Ferric Ammonium Citrate

Motility (Semi-Solid)
Substrate: ❌, Enzyme:❌ , Detection Reagent: ❌
Positive: ❌, Negative: ❌
End Product: Examine growth closely

Urea
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌ (Hint: Sometimes only slant changes color)
End Product: H2O + CO2

Carbohydrate Fermentation
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: Same
End Product: Acid &/or Gas