Enterobeacteriaceae

1

How are Enterobacteraceae classified according to a gram stain?

1

All Enterobacteriaceae ferment glucose

1

Not all members of the Enterobacteriaceae family are cytochrom-oxidase negative.

1

Enterobacteriaceae are obligatory anaerobes

3

Match the Classification with the antigen

Somatic ( O, K, H )
Envelope ( O, K, H )
Flagellar ( O, K, H )

3

Match the Antigen with its description.

Endotoxin, heat stable part of the outer cell wall ( O, K, H )
Heat Labile capsular polysaccharide surrounding the cell wall, covers the O antigen ( O, K, H )
Heat labile protein found in the flagellum of mobile Enterobacteriaceae ( O, K, H )

2

Which Media are usually used for the isolation of Enterobacteriaceae?

1

What reaction would one expect to see on a blood agar if the organism was positive for Hydrogen Sulfide?

4

Match the IMViC test with the importance of the result it generates

E. coli is always positive: If the bacteria is positive in this test it means that the bacteria contains tryptophanase to convert tryptophan to ammonia and indole ( Indole, Methyl Red, Voges-Prokauer, Citrate )
E. Coli is always positive: If the bacteria is positive in this test it means it is able to convert glucose to pyruvic acid ( Indole, Methyl Red, Voges-Prokauer, Citrate )
E. Coli is negative/Klebsiella is Positive: If the bacteria is positive in this test it means that it is able to convert glucose to acetoin ( Indole, Methyl Red, Voges-Prokauer, Citrate )
E. Coli is negative/Kelbsiella is positive. If the bacteria is positive in this test it means that it uses citrate as its main source of carbon ( Indole, Methyl Red, Voges-Prokauer, Citrate )

3

Select the sugars present in Triple Sugar Iron (TSI) agar

1

Which test is used to look for slow/late fermenters?

1

Which test is used to determine if an organism can convert urea into ammonia, carbon dioxide and water?

1

What colour will a Urease test change to if the organism is Urease positive?

2

Match the urea producer will its speed of production

Proetus ( fast producer, slow producer )
Klebsiella ( slow producer, fast producer )

2

Which two organisms will test negative in a motility test?

3

Which organisms will test positive for a Deaminase/Phenylalanine Deaminsae (PAD) test?

3

Match the test colour result with its corresponding conclusion.

Acid Production ❌
No Acid Production ❌
Base production due to use of peptone by organism ❌

Yellow

Red

Pink

1

Which test is a good choice when one wants to distinguish Enterobacteriaceae from gram negative rods that do NOT ferment glucose?

5

Match the colour result from a Triple Sugar Iron (TSI) test with the correct conclusion.

Bacteria ferments glucose but not sucrose or lactose. Alkaline slant over acid deep (K/A). ❌
Bacteria ferments glucose, lactose and/or sucrose. Acid slant over Acid deep (A/A). ❌
Bacteria is not able to ferment glucose. Alkaline Slant over Alkaline deep (K/K) ❌
Production of gas ❌
Bacteria produces Hydrogen Sulfide (H2s) ❌

Red over Yellow

Yellow over Yellow

Red over Red

Cracks or medium pulling away

Black precipitate

1

Which Species of Enterobacteriaceae will produce an acid slant and a deep acid (A/A) result with Gas production and no production of Hydrogen Sulfide in a Triple Sugar Iron (TSI) test.

1

Which species of Enterobacteriaceae will produce an Alkaline slant over a deep acid with positive gas production and positive hydrogen sulfide production in a Triple Sugar Iron (TSI) test?

1

Which species of Enterobacteriaceae will produce an Alkaline slant over a deep acid with negative gas production and negative hyrdogen sulfide production in a Triple Sugar Iron (TSI) test?

1

Which species of Enterobacteriaceae will produce an Alkaline slant over a deep alkalin with negative gas production and negative hyrdogen sulfide production in a Triple Sugar Iron (TSI) test?

Microbiology Quiz sobre Enterobeacteriaceae, criado por Pip Fraggle em 19-09-2016.

Enterobeacteriaceae

How are Enterobacteraceae classified according to a gram stain?

All Enterobacteriaceae ferment glucose

Not all members of the Enterobacteriaceae family are cytochrom-oxidase negative.

Enterobacteriaceae are obligatory anaerobes

Match the Classification with the antigen Somatic O K H( O, K, H ) Envelope O K H( O, K, H ) Flagellar O K H( O, K, H )

Match the Antigen with its description. Endotoxin, heat stable part of the outer cell wall O K H( O, K, H ) Heat Labile capsular polysaccharide surrounding the cell wall, covers the O antigen O K H( O, K, H ) Heat labile protein found in the flagellum of mobile Enterobacteriaceae O K H( O, K, H )

Which Media are usually used for the isolation of Enterobacteriaceae?

What reaction would one expect to see on a blood agar if the organism was positive for Hydrogen Sulfide?

Select the sugars present in Triple Sugar Iron (TSI) agar

Which test is used to look for slow/late fermenters?

Which test is used to determine if an organism can convert urea into ammonia, carbon dioxide and water?

What colour will a Urease test change to if the organism is Urease positive?

Match the urea producer will its speed of production Proetus fast producer slow producer( fast producer, slow producer ) Klebsiella slow producer fast producer( slow producer, fast producer )

Which two organisms will test negative in a motility test?

Which organisms will test positive for a Deaminase/Phenylalanine Deaminsae (PAD) test?

Match the test colour result with its corresponding conclusion. Acid Production ❌ No Acid Production ❌ Base production due to use of peptone by organism ❌

Which test is a good choice when one wants to distinguish Enterobacteriaceae from gram negative rods that do NOT ferment glucose?

Which Species of Enterobacteriaceae will produce an acid slant and a deep acid (A/A) result with Gas production and no production of Hydrogen Sulfide in a Triple Sugar Iron (TSI) test.

Match the Classification with the antigen

Somatic ( O, K, H )
Envelope ( O, K, H )
Flagellar ( O, K, H )

Match the Antigen with its description.

Endotoxin, heat stable part of the outer cell wall ( O, K, H )
Heat Labile capsular polysaccharide surrounding the cell wall, covers the O antigen ( O, K, H )
Heat labile protein found in the flagellum of mobile Enterobacteriaceae ( O, K, H )

Match the urea producer will its speed of production

Proetus ( fast producer, slow producer )
Klebsiella ( slow producer, fast producer )

Match the test colour result with its corresponding conclusion.

Acid Production ❌
No Acid Production ❌
Base production due to use of peptone by organism ❌