16253851
Descrição
Quiz por Olivia Muench, atualizado more than 1 year ago
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Criado por Olivia Muench
aproximadamente 6 anos atrás
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Questão 1
Questão
Microbiology Lab is Biosafety Level 2
Responda
- True
- False
Questão 2
Questão
Bacteria are [blank_start]ubiquitous[blank_end], since they can be found all over and in every environment.
Responda
-
ubiquitous
-
formite
-
thick
Questão 3
Questão
Bacteria have a [blank_start]negative charge[blank_end]. Because of this basic dyes, with their [blank_start]positive charge[blank_end], stain the [blank_start]bacteria[blank_end]. Acidic dyes, with their [blank_start]negative charge[blank_end], stain the [blank_start]background[blank_end].
Responda
-
negative charge
-
positive charge
-
positive charge
-
negative charge
-
negative charge
-
positive charge
-
bacteria
-
background
-
background
-
bacteria
Questão 4
Questão
Examples of acidic dyes:
Responda
-
Eosin
-
Methylene Blue
-
Crystal Violet
-
Nigrosin
-
Safranin
-
Congo Red
-
Maneval's stain
Questão 5
Questão
Which microscope was used in the lab?
Responda
-
Dark-field microscope
-
Phase contrast microscope
-
Bright-field microscope
-
Flourescence microscope
Questão 6
Questão
Limit of Resolution:
D = [blank_start]Wavelength[blank_end] / ([blank_start]NA condensor[blank_end] + [blank_start]NA objective lens[blank_end])
Responda
-
Wavelength
-
NA condensor
-
NA objective lens
-
Wavelength
-
NA condensor
-
NA objective lens
-
Wavelength
-
NA condensor
-
NA objective lens
Questão 7
Questão
Smears should:
Responda
-
withstand washing
-
cover the entire slide
-
not be too thick
-
not result in excessive distortion of the organism
-
fall off the slide
Questão 8
Questão
Purpose of heat-fixing
Responda
-
kill bacteria
-
kill you
-
fix the bacteria to the slide
-
make cells more visible by coagulating cytoplasmic proteins
Questão 9
Questão
Pure cultures should have many different kinds of bacteria
Responda
- True
- False
Questão 10
Questão
Klebsiella Pneumoniae has a capsule
Responda
- True
- False
Questão 11
Questão
Choose what best describes capsules
Responda
-
Made of a polysaccharide layer called glycocalyx
-
Water soluble
-
Fat soluble
-
Heat sensitive
-
Prevents dehydration/dessication
-
Decreases virulence by protecting the cell from phagocytosis
-
Helps attachment to host cell
Questão 12
Questão
Gram-positive bacteria
Responda
-
Have an outer membrane/lipopolysaccharide layer (LPS)
-
Cell wall is ~90% peptidoglycan and has teichoic acid
-
Cell wall is ~10% peptidoglycan
-
are stained pink/red with safranin
Questão 13
Questão
Order of Gram staining:
1. [blank_start]Primary stain[blank_end] with [blank_start]Crystal Violet[blank_end]
2. [blank_start]Mordant[blank_end] with [blank_start]Gram's Iodine[blank_end]
3. [blank_start]Decolorizer[blank_end] with [blank_start]95% Ethanol[blank_end]
4. [blank_start]Counterstain[blank_end] with [blank_start]Safranin[blank_end]
Responda
-
Primary stain
-
Crystal Violet
-
Mordant
-
Gram's Iodine
-
Decolorizer
-
95% Ethanol
-
Counterstain
-
Safranin
Questão 14
Questão
Which describes chemotaxis?
Responda
-
organism vibrates due to molecules hitting the cell
-
directional; independent movement over greater distances
-
movement towards chemical attractants and away from repellants
Questão 15
Questão
Which describes lophotrichous flagella?
Responda
-
flagella on both ends
-
flagella around perimeter of bacteria
-
a single flagella at one end
-
a tuft of flagella at one end
Questão 16
Questão
The motility test media is 0.4-0.5% agar and contains TTC (2,3,5-triphenyltetrazolium chloride)
Responda
- True
- False
Questão 17
Questão
Disadvantages of the motility test:
Responda
-
can be used to test for motility of pathogenic organisms
-
requires incubation time
-
if not performed properly, non-motile organisms appear motile
Questão 18
Questão
TTC is colorless when [blank_start]oxidized[blank_end] and red when [blank_start]reduced[blank_end].
Responda
-
oxidized
-
reduced
Questão 19
Questão
Escherichia coli is motile and Staphylococcus epidermidis is non-motile.
Responda
- True
- False
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