Molecular Biology Techniques

Descrição

Quiz sobre Molecular Biology Techniques , criado por MPusey em 29-12-2014.
MPusey
Quiz por MPusey, atualizado more than 1 year ago
MPusey
Criado por MPusey mais de 9 anos atrás
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Resumo de Recurso

Questão 1

Questão
What monomer units make up the DNA molecule?
Responda
  • Nucleotides
  • Monosaccharides
  • Amino acids
  • Phospholipids

Questão 2

Questão
Which of the four nucleotide bases are purine bases?
Responda
  • Adenine
  • Guanine
  • Cytosine
  • Thymine

Questão 3

Questão
How many carbon rings do purine bases have?
Responda
  • One
  • Two

Questão 4

Questão
Which of the four bases are pyramidine bases?
Responda
  • Adenine
  • Guanine
  • Cytosine
  • Thymine

Questão 5

Questão
How many carbon rings do pyramidine bases have?
Responda
  • One
  • Two

Questão 6

Questão
In which direction do the nucleotide chains run in?
Responda
  • 5' to 3'
  • 3' to 5'

Questão 7

Questão
What type of sequences do restriction enzymes recognize?
Responda
  • Palindromic sequences
  • Repetitive sequences
  • Sequences that only occur once in the whole of our genome

Questão 8

Questão
All restriction enzymes recognize the same sequence. True or false?
Responda
  • True
  • False

Questão 9

Questão
Restriction enzymes that cut in the middle of the palindrome leave....
Responda
  • Blunt ends
  • Sticky ends

Questão 10

Questão
Restriction enzymes that cut at the end of the palindrome leave......
Responda
  • Sticky ends
  • Blunt ends

Questão 11

Questão
What enzyme could be considered the opposite to a restriction enzyme?
Responda
  • RNA polymerase
  • DNA ligase
  • DNA polymerase

Questão 12

Questão
How do restriction enzymes cut DNA?
Responda
  • They hydrolyse the phosphdiester backbone
  • They perform a condensation reaction on the phosphodiester backbone
  • They hydrolyse the hydrogen bonds between DNA bases

Questão 13

Questão
What type of bonds does DNA ligase form in DNA?
Responda
  • Phosphodiester bonds
  • Hydrogen bonds
  • Peptide bonds
  • Triple carbon bonds

Questão 14

Questão
What is the main piece of equipment required for gel electrophoresis?
Responda
  • Agrose gel plate
  • Test tube
  • Restriction enzyme
  • DNA primers
  • Free nucleotides

Questão 15

Questão
What is gel electrophoresis used for?
Responda
  • To determine the size of DNA fragments
  • To map out plasmids
  • To replicate large amounts of DNA

Questão 16

Questão
Are DNA fragments positively or negatively charged?
Responda
  • Positively
  • Negatively

Questão 17

Questão
Which end of the agrose gel plate will the DNA samples move towards?
Responda
  • The positive end
  • The negative end

Questão 18

Questão
Which fragments will move further during gel electrophoresis?
Responda
  • Smaller ones
  • Larger ones

Questão 19

Questão
What are restriction maps used for?
Responda
  • To map out plasmids
  • To determine the size of fragments of DNA
  • To replicate large amounts of DNA
  • To discover new restriction enzymes
  • To isolate restriction enzymes for later use

Questão 20

Questão
If a restriction enzyme cuts a plasmid in one place only, how many fragments of the plasmid will there be in total?
Responda
  • One
  • Two
  • Three
  • Not enough information

Questão 21

Questão
If a restriction enzyme cuts a plasmid in two places only, how many plasmid fragments will there be?
Responda
  • One
  • Two
  • Three
  • Not enough information

Questão 22

Questão
What is Polyermerase Chain Reaction (PCR) used for?
Responda
  • Replicating known sequences of DNA
  • Replicating unknown sequences of DNA
  • Mapping out DNA sequence on a plasmid
  • Cutting DNA at a particular sequence

Questão 23

Questão
What is missing from this list of what is required to complete PCR? Template, 2 primers, free nucleotides, buffer.
Responda
  • DNA polymerase
  • RNA polymerase
  • More primers
  • DNA ligase

Questão 24

Questão
What are the three stages of PCR?
Responda
  • Denaturation
  • Annealing
  • Extension
  • Replication
  • Repetition

Questão 25

Questão
What temperature should denauration take place at in PCR?
Responda
  • 95 degrees
  • 50-65 degrees
  • 72 degrees

Questão 26

Questão
At what temperature should the annealing stage of PCR take place at?
Responda
  • 95 degrees
  • 50-65 degrees
  • 72 degrees

Questão 27

Questão
At what temperature should the extension stage of PCR taken place at?
Responda
  • 90 degrees
  • 50-65 degrees
  • 72 degrees

Questão 28

Questão
If there were four strands of DNA in the PCR before one cycle, how many would there be after the cycle has completed?
Responda
  • Four
  • Eight
  • Sixteen

Questão 29

Questão
What is the purpose of the denaturation stage of PCR?
Responda
  • To separate the strands of DNA
  • To damage the DNA so that it cannot replicate as it would do in the cell
  • To heat the DNA until it is no longer able to code for specific proteins

Questão 30

Questão
What enzyme extends the DNA strand during the extension phase of PCR?
Responda
  • DNA polymerase
  • RNA polymerase
  • DNA ligase

Questão 31

Questão
When would you use cloning instead of PCR to replicate DNA?
Responda
  • When there are more than 1000 base pairs in the sequence
  • When the base sequence is unknown
  • When the base sequence in known
  • When the base sequence contains too many repetitions

Questão 32

Questão
Why is E. coli often used in the cloning process?
Responda
  • Because it grows rapidly in cheap material
  • Because it is the easiest bacteria to make safe for human use
  • Because it is easy to extract the DNA from
  • Because it is easy to re-insert the plasmid into
  • Because it is a very common bacteria
  • Because it has no cell membranes

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