1823989
Questão 1
Questão
What monomer units make up the DNA molecule?
Responda
-
Nucleotides
-
Monosaccharides
-
Amino acids
-
Phospholipids
Questão 2
Questão
Which of the four nucleotide bases are purine bases?
Responda
-
Adenine
-
Guanine
-
Cytosine
-
Thymine
Questão 3
Questão
How many carbon rings do purine bases have?
Responda
-
One
-
Two
Questão 4
Questão
Which of the four bases are pyramidine bases?
Responda
-
Adenine
-
Guanine
-
Cytosine
-
Thymine
Questão 5
Questão
How many carbon rings do pyramidine bases have?
Responda
-
One
-
Two
Questão 6
Questão
In which direction do the nucleotide chains run in?
Responda
-
5' to 3'
-
3' to 5'
Questão 7
Questão
What type of sequences do restriction enzymes recognize?
Responda
-
Palindromic sequences
-
Repetitive sequences
-
Sequences that only occur once in the whole of our genome
Questão 8
Questão
All restriction enzymes recognize the same sequence. True or false?
Responda
- True
- False
Questão 9
Questão
Restriction enzymes that cut in the middle of the palindrome leave....
Responda
-
Blunt ends
-
Sticky ends
Questão 10
Questão
Restriction enzymes that cut at the end of the palindrome leave......
Responda
-
Sticky ends
-
Blunt ends
Questão 11
Questão
What enzyme could be considered the opposite to a restriction enzyme?
Responda
-
RNA polymerase
-
DNA ligase
-
DNA polymerase
Questão 12
Questão
How do restriction enzymes cut DNA?
Responda
-
They hydrolyse the phosphdiester backbone
-
They perform a condensation reaction on the phosphodiester backbone
-
They hydrolyse the hydrogen bonds between DNA bases
Questão 13
Questão
What type of bonds does DNA ligase form in DNA?
Responda
-
Phosphodiester bonds
-
Hydrogen bonds
-
Peptide bonds
-
Triple carbon bonds
Questão 14
Questão
What is the main piece of equipment required for gel electrophoresis?
Responda
-
Agrose gel plate
-
Test tube
-
Restriction enzyme
-
DNA primers
-
Free nucleotides
Questão 15
Questão
What is gel electrophoresis used for?
Responda
-
To determine the size of DNA fragments
-
To map out plasmids
-
To replicate large amounts of DNA
Questão 16
Questão
Are DNA fragments positively or negatively charged?
Responda
-
Positively
-
Negatively
Questão 17
Questão
Which end of the agrose gel plate will the DNA samples move towards?
Responda
-
The positive end
-
The negative end
Questão 18
Questão
Which fragments will move further during gel electrophoresis?
Responda
-
Smaller ones
-
Larger ones
Questão 19
Questão
What are restriction maps used for?
Responda
-
To map out plasmids
-
To determine the size of fragments of DNA
-
To replicate large amounts of DNA
-
To discover new restriction enzymes
-
To isolate restriction enzymes for later use
Questão 20
Questão
If a restriction enzyme cuts a plasmid in one place only, how many fragments of the plasmid will there be in total?
Responda
-
One
-
Two
-
Three
-
Not enough information
Questão 21
Questão
If a restriction enzyme cuts a plasmid in two places only, how many plasmid fragments will there be?
Responda
-
One
-
Two
-
Three
-
Not enough information
Questão 22
Questão
What is Polyermerase Chain Reaction (PCR) used for?
Responda
-
Replicating known sequences of DNA
-
Replicating unknown sequences of DNA
-
Mapping out DNA sequence on a plasmid
-
Cutting DNA at a particular sequence
Questão 23
Questão
What is missing from this list of what is required to complete PCR? Template, 2 primers, free nucleotides, buffer.
Responda
-
DNA polymerase
-
RNA polymerase
-
More primers
-
DNA ligase
Questão 24
Questão
What are the three stages of PCR?
Responda
-
Denaturation
-
Annealing
-
Extension
-
Replication
-
Repetition
Questão 25
Questão
What temperature should denauration take place at in PCR?
Responda
-
95 degrees
-
50-65 degrees
-
72 degrees
Questão 26
Questão
At what temperature should the annealing stage of PCR take place at?
Responda
-
95 degrees
-
50-65 degrees
-
72 degrees
Questão 27
Questão
At what temperature should the extension stage of PCR taken place at?
Responda
-
90 degrees
-
50-65 degrees
-
72 degrees
Questão 28
Questão
If there were four strands of DNA in the PCR before one cycle, how many would there be after the cycle has completed?
Responda
-
Four
-
Eight
-
Sixteen
Questão 29
Questão
What is the purpose of the denaturation stage of PCR?
Responda
-
To separate the strands of DNA
-
To damage the DNA so that it cannot replicate as it would do in the cell
-
To heat the DNA until it is no longer able to code for specific proteins
Questão 30
Questão
What enzyme extends the DNA strand during the extension phase of PCR?
Responda
-
DNA polymerase
-
RNA polymerase
-
DNA ligase
Questão 31
Questão
When would you use cloning instead of PCR to replicate DNA?
Responda
-
When there are more than 1000 base pairs in the sequence
-
When the base sequence is unknown
-
When the base sequence in known
-
When the base sequence contains too many repetitions
Questão 32
Questão
Why is E. coli often used in the cloning process?
Responda
-
Because it grows rapidly in cheap material
-
Because it is the easiest bacteria to make safe for human use
-
Because it is easy to extract the DNA from
-
Because it is easy to re-insert the plasmid into
-
Because it is a very common bacteria
-
Because it has no cell membranes
Quer criar seus próprios Quizzes gratuitos com a GoConqr? Saiba mais.