Questão 1
Questão
The glycolytic pathway in dinosaurs
Responda
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Was likely to be completely different to modern day animals, because every organism has different metabolic pathways
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Was likely to be completely different to modern day organisms because they eat different types of food
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Was likely to be similar or identical to modern day organisms, because metabolic pathways have been highly conserved through evolution
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Is something we cannot make meaningful predictions about because we have no knowledge of how metabolism has evolved
Questão 2
Questão
In thin layer chromatography, ninhydrin can be used to stain which compounds?
Responda
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Monosaccharides
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Amino acids
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Keto acids
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All of the above
Questão 3
Questão
Which of the following lists contains the components of a phospholipid?
Responda
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Choline, phosphate group, glycerol, fatty acids
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Choline, phosphate group, glyceraldehyde, fatty acids
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Carnitine, phosphate group, glycerol, amino acids
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Carnitine, phosphate group, glyceraldehyde, amino acids
Questão 4
Questão
Testosterone belongs to which class of compounds?
Responda
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Fats
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Oils
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Ketose monosaccharides
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Sterols
Questão 5
Questão
Which of the following passes easily through phospholipid bilayers?
Responda
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Chloride ions
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ATP
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Glucose 6-phosphate
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Water
Questão 6
Questão
Peripheral membrane proteins
Responda
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Pass through the cell membrane
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Are non-covalently associated with integral membrane proteins
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Are covalently linked to membrane lipids
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Are always linked to sugars
Questão 7
Questão
Which of the following fatty acids is likely to have the highest melting temperature?
Responda
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One with 14 carbon atoms and no double bonds
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One with 14 carbon atoms and one double bond
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One with 16 carbon atoms and no double bonds
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One with 16 carbon atoms and one double bond
Questão 8
Questão
In the furanose ring form of β-D-fructose, how many of the carbon atoms are chiral?
Questão 9
Questão
Cellulose is a polymer of
Questão 10
Questão
In catabolic (degradative) reactions in humans, the most commonly used oxidising cofactors are
Responda
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NAD+ and FAD
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NADP+ and FAD
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NADH and FADH2
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NADPH and FADH2
Questão 11
Questão
Considering only the glycolytic pathway, what is the net gain in ATP molecules, per molecule of glucose processed?
Questão 12
Questão
Under anaerobic conditions, glycolysis in mammals can result in the compound lactose. Why?
Responda
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To make ATP by substrate level phosphorylation
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To regenerate NAD+
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To make NADH for oxidative phosphorylation
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To replenish Krebs’ (tricarboxylic acid) cycle intermediates
Questão 13
Questão
Which of the following statements about gluconeogenesis is TRUE?
Responda
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Glucose is generated from the hydrolysis of phosphate from glucose 6-phosphate. No ATP is produced in this reaction.
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Glucose is generated from the hydrolysis of phosphate from glucose 6-phosphate. One molecule of ATP is produced in this reaction.
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In mammals, acetyl coenzyme A (AcCoA) can be a starting point.
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The reactions are exactly the same as glycolysis, except in reverse.
Questão 14
Questão
Isomerisation of glucose 1-phosphate to glucose 6-phosphate occurs in
Responda
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Glycolysis and gluconeogenesis
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Glycogen synthesis and degradation
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Glycolysis and the Leloir pathway
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The Leloir pathway and glycogen degradation
Questão 15
Questão
In the Calvin cycle, carbon dioxide reacts with ribulose 1,5-bisphosphate. The first product(s) of this reaction is/are:
Responda
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Two molecules of 3-phosphoglycerate
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Two molecules of glyceraldehyde 3-phosphate
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One molecule of glucose 6-phosphate
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One molecule of fructose 6-phosphate
Questão 16
Questão
In Calvin’s experiments to identify the compounds in the Calvin cycle, the function of the hot ethanol was
Responda
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To shield the algae from the heat of the lamps
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To provide a source of carbon atoms for the algae
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To kill the algae after a defined time point
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To solublise the compounds in the algae
Questão 17
Questão
The main source of energy for sperm cells is
Responda
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Glucose
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Fatty acids
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Glycerol
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Fructose
Questão 18
Questão
The transition state in an enzyme-catalysed reaction:
Responda
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Is always covalently associated to the enzyme.
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Is never covalently associated to the enzyme.
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Can never be observed experimentally.
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Can often be similar in structure to potent enzyme inhibitors.
Questão 19
Questão
In enzyme catalysis, the term ‘approximation’ refers to:
Responda
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A catalytic strategy that facilitates transition state stabilisation through formation of covalent bonds between a substrate and amino acid groups in the active site.
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A catalytic strategy that facilitates transition state stabilisation through formation of hydrogen bonds and electrostatic interactions between a substrate and amino acid groups in the active site.
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A catalytic strategy that facilitates transition state stabilisation through interaction with metal ions in the active site.
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A catalytic strategy that facilitates transition state stabilisation through direct transfer of a proton to the substrate from an acidic amino acid group in the active site.
Questão 20
Responda
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Is completely located in the mitochondrial matrix – preventing the exposure of free ammonium to other components of the eukaryote cell.
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Allows free ammonia obtained directly from de-amination of glutamate to be converted to urea – preventing the exposure of free ammonium to other components of the eukaryote cell.
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Allows free ammonia obtained directly from de-amination of tryptophan to be converted to urea – preventing the exposure of free ammonium to other components of the eukaryote cell.
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Is completely located in the cytoplasm of the cell – preventing the exposure of free ammonium to other components of the eukaryote cell.
Questão 21
Questão
Serine proteases:
Responda
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Are proteases that hydrolyse polypeptides with serine in the F1 position
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Are proteases that are found in the cytoplasm of all cells
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Utilise a serine residue at the active site to facilitate substrate binding
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Utilise a serine residue at the active site to facilitate cleavage of peptide bonds
Questão 22
Questão
Consider an enzyme that shows Michaelis-Menton kinetics where:
vo = Vmax . [S] / (Km + [S])
If a substrate, S, is present at a concentration of 4 mM, and Km is 2 mM, the rate of reaction (vo) measured will be:
Responda
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Half of Vmax
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Two thirds of Vmax
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Double Vmax
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Three times Vmax
Questão 23
Questão
In acid-base catalysis:
Responda
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An acidic- or basic- amino acid in the active site of an enzyme facilitates transition state formation by hydrogen abstraction from an appropriate substrate.
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An acid- or basic- substrate in the active site of an enzyme facilitates transition state formation by hydrogen abstraction from a catalytic amino acid in the active site.
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Both are correct.
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Neither are correct.
Questão 24
Questão
The protein ubiquitin:
Responda
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Can be covalently linked to proteins via the N-terminus glycine residue.
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Is a polypeptide.
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Is an essential component of eukaryote respiratory chains.
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Can be covalently linked to proteins via isopeptide bond formation.
Questão 25
Questão
An oxyanion hole is:
Responda
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A region of the enzyme active site that facilitates binding of positively charged substrates through their association with oxygen-containing amino-acid side chains in the enzyme.
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A region of the enzyme active site that facilitates binding of negatively charged substrates through their association with oxygen-containing amino acid side chains in the enzyme.
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A region of the active site that facilitates binding of positively charged oxygen containing groups present in a substrate.
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A region of the active site that facilitates binding of negatively charged oxygen-containing groups present in a substrate.
Questão 26
Questão
If the ΔG°′ of the reaction Malate → Oxaloacetate is +30 kJ/mol, what will happen in the presence of malate dehydrogenase under standard conditions?
Responda
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The reaction will proceed fast with the formation of the explosive products.
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The reaction will not occur spontaneously.
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The reaction will never reach equilibrium.
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The reaction will proceed spontaneously from left to right.
Questão 27
Questão
Which of the following redox couple have the highest midpoint redox potential?
Questão 28
Questão
What is the source of energy for ATP synthesis in mitochondria?
Responda
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AMP, ADP and inorganic phosphate
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Gradient of Na+ across the inner mitochondrial membrane
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Matrix fumarase and aconitase
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None of the above
Questão 29
Questão
Which of the following cannot reduce mitochondrial respiratory chain in normal conditions?
Responda
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NADH
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Oxygen
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FADH2
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Ubiquinol
Questão 30
Questão
The oxygen molecules released into the air as a product of photosynthesis comes from:
Responda
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Light quanta
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Cytochome c oxidase
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Carbon dioxide
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Water
Questão 31
Questão
Production of lactic acid in anaerobic muscle cells is:
Responda
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Caused by breakdown of glycogen
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Caused by a buildup of AMP
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Coupled to the recycling of NAD+ to NADH by lactate dehydrogenase when it oxidises pyruvate
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Due to pyruvate being reduced by lactate dehydrogenase while it recycles NADH to NAD+
Questão 32
Questão
Phosphofructokinase-1 activity is:
Questão 33
Questão
The Warburg effect:
Responda
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Is the inhibition of glycolysis that occurs in anaerobic cells when exposed to oxygen
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Is the continued use of anaerobic fermentation by cancer cells even in the presence of oxygen
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Is caused by an over-active glycerol phosphate shuttle in cancer
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Is due to the inhibition of phosphofructokinase-1 by AMP
Questão 34
Questão
Glycogen synthase activity is:
Questão 35
Questão
Phosphofructokinase-1 activity is:
Questão 36
Questão
Which of the following is NOT true of Aldosterone?
Questão 37
Questão
Calcitonin is a hormone that is released from:
Responda
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The pituitary gland
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The thyroid gland
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The hypothalamus
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The parathyroid gland
Questão 38
Questão
Which of the following is NOT a feature of metabolic pathways?
Responda
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Sequential feedback inhibition
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Common intermediate metabolites
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The presence of aquaporins
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End product inhibition
Questão 39
Questão
Which of the following is NOT true of the anabolic hormone insulin?
Responda
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It increases glycogen synthesis
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It increases fatty acid synthesis
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It increases esterification of fatty acids
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It increases protein breakdown (proteinolysis)
Questão 40
Questão
Which of the following has NOT been a commercial method for the synthesis of artificial human insulin?
Responda
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Separate A- and B-chain synthesis in E. coli
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Phage display in E. coli
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Pro-insulin synthesis in E. coli
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Biosynthesis in brewer’s yeast (Saccharomyces cerevisiae)