DNA (labeling) for biochem and cell biology (lecture 2)

Descrição

1st year Biochemistry and molecular biology Quiz sobre DNA (labeling) for biochem and cell biology (lecture 2), criado por MrSujg em 29-11-2015.
MrSujg
Quiz por MrSujg, atualizado more than 1 year ago
MrSujg
Criado por MrSujg quase 9 anos atrás
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Resumo de Recurso

Questão 1

Questão
Label the image
Responda
  • 1'
  • 2'
  • 3'
  • 4'
  • 5'
  • alpha
  • Beta
  • gamma
  • base

Questão 2

Questão
What base is it ?
Responda
  • Adenine
  • Guanine
  • Cytosine
  • Thymine
  • Uracil

Questão 3

Questão
What base is it ?
Responda
  • Guanine
  • Adenine
  • Cytosine
  • Thymine
  • Uracil

Questão 4

Questão
What base is it?
Responda
  • Cytosine
  • Adenine
  • Guanine
  • Thymine
  • Uracil

Questão 5

Questão
What base is it?
Responda
  • Thymine
  • Adenine
  • Uracil
  • Cytosine
  • Guanine

Questão 6

Questão
What base is it?
Responda
  • Uracil
  • Adenine
  • Guanine
  • Tymine
  • Cytosine

Questão 7

Questão
Identify primers
Responda
  • 3'
  • 5'

Questão 8

Questão
Label the image
Responda
  • 5'
  • 3'
  • 5'
  • 3'
  • alpha
  • beta
  • gamma
  • O
  • C
  • S
  • H
  • N
  • F
  • DNA polymerase I
  • DNA polymerase II
  • DNA polymerase III

Questão 9

Questão
Label the image
Responda
  • Minor groove
  • Major groove

Questão 10

Questão
Label the image
Responda
  • Guanine
  • Cytosine
  • Thymine
  • Adenine

Questão 11

Questão
Determine the primers
Responda
  • 5'
  • 3'
  • 3'
  • 5'
  • 3'
  • 5'
  • 5'
  • 3'

Questão 12

Questão
Label the image
Responda
  • A-DNA
  • B-DNA
  • Z-DNA

Questão 13

Questão
Label the image
Responda
  • Conservative mechanisms
  • Semiconservative mechanism

Questão 14

Questão
Label the image
Responda
  • Origin
  • Growing fork

Questão 15

Questão
Label the image
Responda
  • EcorRI restriction site
  • Origin
  • Replication bubble
  • Time of replication

Questão 16

Questão
Labelt the replication cartoon
Responda
  • Primase
  • SIngle0stranded region of parent DNA
  • RNA primer
  • DNA polymerase III
  • Newly made DNA
  • DNA polymerase I
  • RNA still to be replaced
  • Newest DNA
  • Completed daughter cell

Questão 17

Questão
Label the cartoon showing the unwinding at the origin
Responda
  • 9-mers
  • Negatively supercoiled template
  • DnaA
  • ATP
  • Initial complex
  • Open complex
  • DnaC
  • DnaB (helicase)
  • Prepriming complex

Questão 18

Questão
Label the cartoon showing Simultaneous synthesis on both strands
Responda
  • Point of joining
  • Lagging strand
  • Okazaki fragment
  • Short RNA primer
  • Leading strand
  • Parental DNA duplex
  • Daughter duplex
  • Direction of fork movement

Questão 19

Questão
Label the cartoon showing the participitation of topoisomers in DNA unwinding
Responda
  • Covalently closed circular template
  • DNA replication
  • Unwound parental duplex
  • Negative supercoils
  • Positive supercoils
  • Overwound region

Questão 20

Questão
Label DNA polymerase
Responda
  • Fingers
  • Thumb
  • Growing strand
  • Template strand
  • Exo
  • Palm
  • Pol

Questão 21

Questão
Label the image showing mismatch repair
Responda
  • Daughter strand
  • Parental strand
  • Mismatched base pair
  • Mismatch recognition
  • MutS
  • MutH
  • MutH cleavage
  • Gap repair by DNA polymerase and ligase

Questão 22

Questão
Label the cartoon showing excision repair
Responda
  • Initial damage recognition
  • 23B
  • XP-C
  • Opening of DNA double helix
  • TFIIH
  • XP-G
  • RPA
  • XP-F and XP-G endonucleases
  • DNA polymerase and DNA ligase

Questão 23

Questão
Label the image showing PCR
Responda
  • DNA Denaturation, annealing of primers
  • Elongation of primers
  • DNA denaturation; annealing or primers 2
  • Elongation of primers 2

Questão 24

Questão
Label the image
Responda
  • RNA polymerase I
  • RNA polymerase II
  • RNA polymerase 3

Questão 25

Questão
Label the image showing the Transcription in Prokaryotes
Responda
  • Promoter DNA
  • -35 sequence
  • 10 sequence
  • Coding strand
  • Template strand
  • Startpoint

Questão 26

Questão
Labelt the image showing A-Binding Domains (DBDs)
Responda
  • Helix-turn-helix motif
  • The lambda repressor
  • Zinc finger motif
  • Leucine zipper motif
  • Helix-loop-helix motif

Questão 27

Questão
Label the image showing the lac operon activation
Responda
  • Beta-galactosidase
  • RNA polymerase bound to promoter
  • lac-mRNA
  • Lactose
  • RNA polymerase transcribing the operon
  • lacI gene
  • Inactive form of repressor

Questão 28

Questão
Label the image showing the regulation of the lac Operon
Responda
  • Promoter
  • CAP site
  • CAP
  • a70-Pol
  • lac repressor
  • Lactose
  • cAMP

Questão 29

Questão
Label the image showing Eukaryotic promoters
Responda
  • GC box
  • CAAT box
  • TATA box
  • Proximal control elements
  • Core promoter
  • Start of transcription
  • Poly (A) site
  • 5' untranslated region (leader)
  • 3' untranslated region (trailer)

Questão 30

Questão
label the image showing General Eukaryotic Transcription Factors
Responda
  • Core promoter
  • Startpoint
  • TFIID
  • TFIIA
  • TFIIB
  • TFIIF
  • RNA polymerase 2
  • TFIIE
  • TFIIH
  • Transcription begins

Questão 31

Questão
Label the image showing Additional Eukaryotic Transcription Factors
Responda
  • Enhancer
  • Core promotor
  • TATA
  • Inr
  • Gene
  • Activators
  • Coactivators
  • Enhanceosome
  • TFIID
  • Activator and Coactivator
  • Transcription

Questão 32

Questão
label the image showing Tissue-Specific Gene Expression
Responda
  • Control elements
  • Core promoter
  • Regulatory transcription factors
  • General transcription factors
  • RNA polymerase
  • Transcription complex
  • DNA
  • RNA transcript

Questão 33

Questão
Label the image showing how the final mRNA is made
Responda
  • Exon
  • Intron
  • Poly (A) site
  • Termination sites
  • Transcription 5' capping
  • Cleavage at poly (A) site
  • Polyadenylation
  • RNA splicing

Questão 34

Questão
Labe the image showing Microarrays: Genome-Wide Analysis
Responda
  • RNA isolation
  • Reverse transcriptase labeling
  • Combine targets
  • Hybridaze to microarray

Questão 35

Questão
Label the image showing the molecular Dogma
Responda
  • Transcription
  • Translation
  • Assembly
  • tRNA
  • rRNA
  • Ribosomes
  • Polypeptides
  • Proteins
  • Ribosomal proteins

Questão 36

Questão
Label the image
Responda
  • Primary RNA transcript
  • 5'
  • 3'
  • DNA
  • Promoter
  • Transcription
  • Coding sequence
  • Upstream
  • Downstream

Questão 37

Questão
Follow the genetic code to match the appropriate amino acid to each codon
Responda
  • Ala
  • Cys
  • Leu
  • Arg
  • Ile
  • Val
  • Tyr
  • Glu

Questão 38

Questão
Label the type of the mutation
Responda
  • Frameshift
  • Missense
  • Nonsense

Questão 39

Questão
Identify the type of ribosome, and the size of the assembled unit
Responda
  • Prokaryotic
  • Eukaryotic (vertebrate)
  • 70S
  • 80S

Questão 40

Questão
Label the image showing aminoacyl-tRNAsynthetases
Responda
  • High-energy ester bond
  • Amino acid (Phe)
  • mRNA
  • Aminoacyl-tRNA
  • tRNA specific for Phe

Questão 41

Questão
Label the image
Responda
  • Adenosine deaminase
  • Adenosine
  • Inosine

Questão 42

Questão
Label the initiator methionine
Responda
  • Formyl group
  • Carboxyl group
  • Acetyl group
  • Phosphate group

Questão 43

Questão
Label each mRNA
Responda
  • Prokaryotic mRNA
  • Eukaryotic mRNA

Questão 44

Questão
Label the ribosome sites
Responda
  • A site
  • P site
  • E-site
  • mRNA-binding site
  • mRNA
  • Growing peptide chain

Questão 45

Questão
Label the image showing translation
Responda
  • P site
  • E site
  • A site
  • Release factor
  • free tRNA
  • Polypeptide
  • Ribosomal subunits
  • mRNA

Questão 46

Questão
Label the image showing translation mechanism
Responda
  • AUG start codon
  • UAG stop codon
  • mRNA
  • Initiation
  • Elongation
  • Termination
  • Release factor
  • Completed polypeptide
  • Recycling of translational components
  • Ribosomal subunits

Questão 47

Questão
Label the image showing western blotting
Responda
  • Electrophoresis and transfer
  • Antibody detection
  • Chromogenic detection

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