449175
Descrição
Quiz por gina_evans0312, atualizado more than 1 year ago
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Criado por gina_evans0312
mais de 10 anos atrás
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Questão 1
Questão
Define Protein Folding
Responda
-
The process by which a protein obtains its natural 3D state
-
The process by which a polypeptide chain is created from mRNA
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The process by which DNA sequences are converted into an mRNA chain
Questão 2
Questão
Which structure of a protein shows biological activity?
Responda
-
Primary
-
Secondary
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Tertiary
Questão 3
Questão
Which of the following occurs to ALL proteins?
Responda
-
Non-covalent folding
-
Covalent folding
Questão 4
Questão
Which of the following are covalent modifications of the polypeptide chain?
Responda
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Glycosylation
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Phosphorylation
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Formation of disulphide bonds
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Van Der Waals interactions
-
Electrostatic interactions
Questão 5
Questão
Folding corresponds to a hierarchy of protein structure
Responda
- True
- False
Questão 6
Questão
Put the following in Order
A -Unfolded
B - Nucleation
C - Secondary Structure
D - Domain
E - Active Oligomer
F - Inactive Oligomer
G - Supersecondary Structure
H - Folded Monomer
Responda
-
A-B-C-G-D-H-F-E
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A-B-E-F-G-D-C-H
-
A-D-C-B-E-F-H
Questão 7
Questão
What is meant by the 'Thermodynamic Hypothesis of Protein Folding'
Responda
-
That proteins fold into different structures based on the energy available to them in the medium
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That the information for correct protein folding is found within the sequence itself
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That the protein will always fold into the lowest possible energy state
Questão 8
Questão
The Kinetic Hypothysis suggests that there is a specific folding pathway for each protein- protein folding is not random
Responda
- True
- False
Questão 9
Questão
Describe the features of the RNAase Refolding experiment?
Responda
-
RNAase is denatured with urea
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RNAase is denatured with detergent
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And an oxidising agent to remove the disulphide bridges
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And a reducing agent to remove the disulphide bridges
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Once the denaturing agents were removed and it was re-oxdised
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Once the denaturing agents were removed and it was re-reduced
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Protein fully reformed itself and worked
Questão 10
Questão
How do we know the RNAase really was denatured during the experiment?
Responda
-
If the protein was allowed to reform before the reducing agents were removed, the sequence showed non native sulphides and didn't work
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We don't know- we can never really KNOW anything
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If the protein was allowed to reform before the reducing agents were removed, the sequence didn't work
Questão 11
Questão
Which of the following are conditions for In Vitro Refolding (Like in the RNAase experiment)?
Responda
-
Structure must be amenable to environmental changes
-
Free energy path must be relatively smooth
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Must contain covalent bonds
-
Must have slow transitions
-
Structure must have unique free energy
Questão 12
Responda
-
Cis
-
Trans
Questão 13
Questão
Trans formations are less stable due to their steric hindrance
Responda
- True
- False
Questão 14
Questão
Proline is added to an amino acid chain exclusively in what form?
Responda
-
Trans
-
Cis
-
The other form occurs 10-40% of the time, and must be altered post addition
Questão 15
Questão
Which of the following proteins converts cis isomers to their trans form?
Responda
-
Peptidyl Propyl Isomerases
-
Peptidyl Glucyl Isomerases
-
Peptidyl Lipyl Isomerases
Questão 16
Questão
In the Cis form, the clash of functional groups around the alpha carbon is much greater due to steric hindrance
Responda
- True
- False
Questão 17
Responda
-
Disulphide
-
Van Der Waals
-
Electrostatic Attraction
Questão 18
Questão
The previous amino acid to proline experiences steric hindrance in either proline conformation
Responda
- True
- False
Questão 19
Questão
Cis proline has _ energy than/to trans proline
Responda
-
Greater
-
Less
-
Equal
Questão 20
Questão
The two cystine molecules that bind together are known as what?
Responda
-
Cysteine
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Bi-cystine
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Bi-sulphur cystine
Questão 21
Questão
Proteins with di-sulphide bonds are most likely to be found where?
Responda
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In low/high pH
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Extracellular proteins
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Cytoplasmic proteins
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In low/high salt concentrations
Questão 22
Questão
Phosphorylation occurs on which residues?
Responda
-
Serine
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Valine
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Threonine
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Tyrosine
-
Glutamine
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Asparagine
Questão 23
Questão
Pro-sequences are found at the C-terminus
Responda
- True
- False
Questão 24
Questão
The Pro-sequence is also known as
Responda
-
A chaperone sequence
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A ligation sequence
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A glycosylation sequence
Questão 25
Questão
Which proteins will have a pro-sequence?
Responda
-
Chaperones
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Proteases
-
Ligand-binding proteins
Questão 26
Questão
The protein with a pro-sequence will auto-cleave it before folding correctly
Responda
- True
- False
Questão 27
Questão
What is co-operativity in protein folding?
Responda
-
Where one area of the protein that has already folded provides a nucleation site for another domain to fold
-
The pre-formed proteins will be used as a template to fold more of that species
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Where chaperone proteins assist in protein folding
Questão 28
Questão
What does the Hydrophobic Zipper Model suggest?
Responda
-
That hydrophobic amino acids in close proximity are more likely to interact
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That hydrophillic amino acids in close proximity are more likely to interact
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Constraining the chain and making other residues more likely to interact, which further constrains the chain, etc.
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