452186
Descrição
Quiz por gina_evans0312, atualizado more than 1 year ago
|
Criado por gina_evans0312
aproximadamente 11 anos atrás
|
|
Questão 1
Questão
Regulatory modifications can be removed
Responda
- True
- False
Questão 2
Questão
What is added in Palmitoylation?
Responda
-
Palmitic Acid
-
Palmitoyl-5-Phosphate
-
Palmitite
Questão 3
Questão
The substrate in palmitoylation is added through a thio-ester bond
Responda
- True
- False
Questão 4
Questão
Where is the substrate in palmitoylation added?
Responda
-
The central cystine
-
The two flanking leucines
-
Of C/LCL/C
Questão 5
Questão
Describe Model 1 of regulatory modification
Responda
-
The modification changes the functional state (Active --> Inactive or vice versa)
-
The modification changes the affinity of the protein for its binding partner
Questão 6
Questão
In Model 2, what does the addition do?
Responda
-
Activates/deactivates the protein
-
Creates a new binding site that only exists with the modification
-
Marks the protein for destruction
Questão 7
Questão
Phosphorylation is the least used modification in eukaryotic cells
Responda
- True
- False
Questão 8
Questão
What is the donor for phosphorylation?
Responda
-
Gamma phosphate of ATP
-
Alpha phosphate of ATP
-
Beta phosphate of ATP
Questão 9
Questão
Name the two enzymes that add/remove the phosphate
Responda
-
Protein kinases
-
Protein phosphatases
-
Protein isomerases
-
Protein transferases
Questão 10
Questão
Phosphates are added to amino acids with OH groups on the R chain
Responda
- True
- False
Questão 11
Questão
Phosphorylation can be used for both Model 1 & 2
Responda
- True
- False
Questão 12
Questão
Give an example of a Model 1 phosphorylation
Responda
-
Phosphorylation of Erk Kinase
-
Phosphorylation of tyrosine kinase
-
Phosporylation of Ras
Questão 13
Questão
Describe a Model 2 pathway for phosphorylation
Responda
-
Platelet derived growth factor self phosphorylates on Tyr
-
Allows binding of other proteins SH2 domains
-
Phosphorylation of Raf
-
Phosphorylation of MEK
Questão 14
Questão
Acetylation is used almost exclusively for model 1
Responda
- True
- False
Questão 15
Questão
To which residue is the substrate for Acetylation added?
Responda
-
Lysine
-
Leucine
-
Tyrosine
Questão 16
Questão
Acetylysine is neutral
Responda
- True
- False
Questão 17
Questão
Acetyl-lysines are recognised by what?
Responda
-
Bromo-domains
-
Phosphoserine domains
-
KH4 domains
Questão 18
Questão
The donor for Acetylation is?
Responda
-
N-acetyl glucosamine
-
N-acetyl galactosamine
-
Acetyl CoA
Questão 19
Questão
Name the proteins that add/remove the substrate in acetylation
Responda
-
Acetyl Transferase
-
Deacetylases
-
Acetyl Isomerase
-
Acetylase
Questão 20
Questão
How is acetylation used for DNA density regulation?
Responda
-
The histone monomers (H3 & H4 especially) have tails rich in lysine for acetylation
-
T/A residues are acetylated, which signals to move them to the histone and be silenced
-
GTG repeats are acetylated to activate them, moving them away from the histone
Questão 21
Questão
Acetylation causes the DNA to relax
Responda
- True
- False
Questão 22
Questão
What is the role of bromodomains?
Responda
-
To interact with histones
-
To interact with nucleosoems
-
Bring in other proteins for the chromatin remodelling complex
Questão 23
Questão
What is the donor substrate for methylation?
Responda
-
S-adenosyl Methionine
-
Ethanol
-
N-acetyl methionine
Questão 24
Questão
Name the two protein sets that add/remove methyl groups
Responda
-
Methyl transferase
-
Demethylase
-
Methyl isomerase
-
Methylases
Questão 25
Questão
There are separate enzymes for lysine and arginine methylation
Responda
- True
- False
Questão 26
Questão
Methyl can be added to the same lysine how many times?
Responda
-
3
-
4
-
5
Questão 27
Questão
MeK, Me2K and Me3K are all created and recognised by the same enzyme
Responda
- True
- False
Questão 28
Questão
Some enzymes can mono/bi/ and tri methylate, some can only do one
Responda
- True
- False
Questão 29
Questão
Name the forms of dimethylated arginine
Responda
-
Me2R
-
Symmetrical Me2R
-
Asymmetrical Me2R
Questão 30
Questão
What's the difference between s & aMe2R
Responda
-
One is in Cis, the other is in trans formation
-
aMe2R has both methylations on the same arginine
-
aMe2R has both methylations on different arginines
Questão 31
Questão
Arginine has 5 nitrogens to be replaced
Responda
- True
- False
Questão 32
Questão
Tudor domains recognise methylation
Responda
- True
- False
Questão 33
Questão
Why is S-adenosyl methionine used as the donor
Responda
-
It has a 5-bonded carbon, which makes the methyl easy to remove
-
It has a 4-bonded phosphate, which makes the methyl easy to remove
-
It has a 3-bonded sulphur, which makes the methyl easy to remove
Questão 34
Questão
Tandem tudor cannot bind to Me3K as there is no longer a H for it to bind with
Responda
- True
- False
Questão 35
Questão
A type of tudor domain will recognise asymmetical Me2R
Responda
- True
- False
Questão 36
Questão
ADP ribosylation is mostly Model 2
Responda
- True
- False
Questão 37
Questão
What is the donor for ADP ribosylation?
Responda
-
NAD+
-
FAD+
-
NADH
Questão 38
Questão
How is the ADP gained from the substrate?
Responda
-
Cut off the nicotinamide and you're left with the ADP
-
Cut off the flavin and you're left with the ADP
Questão 39
Questão
Name the proteins that add/removed ADP
Responda
-
Poly-ADP Ribose Polymerase
-
ADP Transferase
-
De-ADPase
-
Poly-ADP Ribose Glycohydrolases
Questão 40
Questão
Which amino acids are the ADP(s) added to?
Responda
-
Lysine
-
Glutamine
-
Valine
-
Glutaminc Acid
Questão 41
Questão
Which amino acid has the ADP(s) added?
Responda
-
Lysine
-
Glutamine
-
Valine
Questão 42
Questão
ADP ribose can be addded to ADP ribose in straight or branched chains
Responda
- True
- False
Questão 43
Questão
What is the role of glutamic acid ADP ribosylation?
Responda
-
Happens to linker histones
-
Happens to open stretches of DNA
-
Recognised by DNA damage response proteins
-
Recognised by silencing proteins
Questão 44
Questão
Name the 3 enzymes used in the addition of Ubiquitin
Responda
-
E1- Ubiquitin Conjugating Enzyme
-
E1- Ubiquitin Activating Enzyme
-
E2- Ubiquitin Conjugating Enzyme
-
U2- Ubiquitin Ligase
-
U3- Ubiquitin Activating Enzyme
-
U3- Ubiquitin Ligase
Questão 45
Questão
De-Ubiquitinating Enzyme removes Ubiquitin
Responda
- True
- False
Questão 46
Questão
What type of bond forms between Ubiquitin and the target residue?
Responda
-
Thio-ester, between the COOH of the ubiquitin and the lysine on the target protein
-
Isopeptide, between the COOH of the ubiquitin and the lysine on the target protein
-
Disulphide, between the COOH of the ubiquitin and the lysine on the target protein
Questão 47
Questão
Ubiquitinated lysine 63 are open and flexible and acts a signalling site for complex formation
Ubiquitinated lysine 48 are compact and mark the protein for destruction
Responda
- True
- False
Questão 48
Questão
What is the first step of ubiquitination?
Responda
-
Ubiquitin is activated by ATP hydrolysis
-
Ubiquitin is activated by GTP hydrolysis
-
Ubiquitin is activated by UTP hydrolysis
Questão 49
Questão
Once Ub has been activated, what enzyme does it bind to, and what bond forms?
Responda
-
Thio-ester
-
Peptide
-
E1
-
E2
Questão 50
Questão
The Ub is then passed on to..
Responda
-
E1
-
E2
-
E3
Questão 51
Questão
What is the role of E3?
Responda
-
Binding of an E2-Ub complex
-
Binding on an E1-Ub complex
-
Binds to substrate protein & transfers ubiquitin to amino group of lysine
Questão 52
Questão
Protein specificity for Ubiquitination lives in E2
Responda
- True
- False
Questão 53
Questão
How many versions of E1 are there?
Responda
-
1
-
2
-
3
Questão 54
Questão
There are 35 versions of E2
Responda
- True
- False
Questão 55
Questão
How many E3's are there?
Responda
-
1
-
10's
-
100's
Quer criar seus próprios Quizzes gratuitos com a GoConqr? Saiba mais.