Natural Products

Descrição

a quiz on natural product extraction and tests
Sam Watkins
Quiz por Sam Watkins, atualizado more than 1 year ago
Sam Watkins
Criado por Sam Watkins mais de 8 anos atrás
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Resumo de Recurso

Questão 1

Questão
In specimen collection the plant must be dried and kept sterile to minimise damage
Responda
  • True
  • False

Questão 2

Questão
Which of the following is best to aid identification of the natural product/plant
Responda
  • plant handbook
  • flora or field guide
  • herbarium

Questão 3

Questão
macerated leaves are treated with chemicals that gradually decrease in polarity to extract chemicals
Responda
  • True
  • False

Questão 4

Questão
General screening bioassays are [blank_start]simple[blank_end], [blank_start]inexpensive[blank_end] and used for [blank_start]rapid screening[blank_end] of extracts
Responda
  • simple
  • complex
  • inexpensive
  • expensive
  • rapid screening
  • specific enzyme screening

Questão 5

Questão
specialised screening bioassays are [blank_start]subcellular[blank_end] assays directed at [blank_start]specific[blank_end] enzymes or receptors. Compounds working by mechanisms unrelated to the assay are likely to be missed.
Responda
  • subcellular
  • multicellular
  • specific
  • broad spectrum

Questão 6

Questão
which of the following is FALSE
Responda
  • Brine shrimp lethality tests are used to determine cytotoxicity (potential antitumour) of extracts
  • crown gall tumour inhibition tests give a fairly accurate prediction of in vivo murine antileukemic activity
  • starfish or sea urchin assays are used to investigate in vivo antileukemic activity
  • agar dilution streak assay means up to 7 microorganisms can be screened simultaneously on a petri dish
  • microtitre plates allow testing of 3 dilutions of one or two extracts against up to 24 microorganisms

Questão 7

Questão
crown gall tumour inhibition tests use potatoes to measure inhibition of growth
Responda
  • True
  • False

Questão 8

Questão
which 3 do starfish or sea urchin assays allow detection of?
Responda
  • dna and rna synthesis inhibitors
  • mRNA substrate inhibitors
  • microtubule assembly inhibitors
  • protein synthesis inhibitors

Questão 9

Questão
which best applies to an agar dilution streak assay?
Responda
  • up to 5 micro-organisms can be tested
  • up to 4 micro-organisms can be tested
  • up to 7 micro-organisms can be tested

Questão 10

Questão
Which tlc staining technique is used to test acidic groups
Responda
  • bromocrescol green
  • vanillin
  • potassium permanganate

Questão 11

Questão
which one of the following is not considered in NP isolation or synthesis
Responda
  • source - availability
  • environmental and local impact
  • cost
  • complexity of novel structures
  • diluent needed

Questão 12

Questão
What are the stages of natural product processing? [blank_start]Specimen[blank_end] collection, establishing [blank_start]taxonomy[blank_end], extracting possible active molecules, using [blank_start]screening[blank_end] techniques to evaluate therapeutic activity, identifying and [blank_start]isolating[blank_end] the structure responsible for the activity, using organic synthesis
Responda
  • Specimen
  • Taxonomy
  • molecules
  • structure
  • taxonomy
  • specimen
  • molecules
  • therapeutics
  • screening
  • showing
  • therapeutic
  • herbariums
  • isolating
  • burning
  • melting
  • releasing

Questão 13

Questão
a range of techniques are used to isolate active compounds (fractionation). these include [blank_start]TLC[blank_end], column chromatography, [blank_start]HPLC[blank_end] and combinations such as HPLC/MS
Responda
  • TLC
  • LTC
  • MSMS
  • HPLC
  • ELISA
  • SPE

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