Question 1
Question
How do we study microbes and all the jazz?
Answer
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Culture them
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Ingest them
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Look at them under a microscope
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Study their DNA, RNA, and proteins!
Question 2
Question
Complex media is harder to make and every single ingredient is measured.
Question 3
Question
General Media has which of the following characteristics?
Answer
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Super selective
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Great Plate Count Anomaly
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Still can’t culture everything
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Cultures as many microbes as possible
Question 4
Question
Enrichment media has which of the following characteristics/example(s)?
Question 5
Question
[blank_start]Selective[blank_end] media selects for certain bacteria and selects against other bacteria.
Answer
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Selective
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Enriched
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Differential
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General
Question 6
Question
Differential media usually is dependent on bacterial metabolic activity.
Question 7
Question
Differential media is not similar or at all like selective media.
Question 8
Question
Choose the correct terms for the following characteristics of autoclaving.
Autoclaving uses high [blank_start]pressure[blank_end] and high temperature to kill even [blank_start]endospores[blank_end].
[blank_start]Wet[blank_end] heat has more energy than dry heat. This kills everything but can be [blank_start]bad[blank_end] for delicate or reactive compounds. Autoclaving can also cause [blank_start]vitamins[blank_end] to [blank_start]denature[blank_end]. Also, trace metals that are [blank_start]redox[blank_end]-sensitive might react and become unavailable.
Answer
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pressure
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endospores
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Wet
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bad
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vitamins
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denature
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redox
Question 9
Question
If autoclaving can denture vitamins and ruin or cause trace metal react, then what is another method of sterilization that would be better suited to handle this problem?
Question 10
Question
Which of the following is TRUE about filtered sterilization?
Answer
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Used for reactive compounds (vitamins or trace metals)
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Removes the cells from the liquid, as autoclaving kills cells but doesn’t necessarily destroy them.
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Requires UV lamp
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Need cell-free water to do dilutions for microscope counts
Question 11
Question
Which is not a form of UV light sterilization?
Question 12
Question
Order the strength of UV light sterilization in increasing order in terms of effectiveness of killing microbes:
1) Lowest --> [blank_start]UV-A[blank_end]
2) Medium --> [blank_start]UV-B[blank_end]
3) Highest --> [blank_start]UV-C[blank_end]
Answer
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UV-A
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UV-B
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UV-C
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UV-B
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UV-A
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UV-C
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UV-C
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UV-A
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UV-B
Question 13
Question
UV-A is the least effective in killing microbes but has the deepest penetrance. UV-C is the most effective in killing microbes but has the lowes penetrance.
Question 14
Question
Hypothermiphiles and thermophiles grow in [blank_start]hot[blank_end] environments.
Psychrophiles grow in [blank_start]cold[blank_end] environments.
Halophiles love [blank_start]salt[blank_end].
Osmophiles love [blank_start]sugar[blank_end].
Xerophiles grow in [blank_start]dry[blank_end] environments.
Question 15
Question
What is true about thermophiles?
Answer
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They love cold environments
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They have unsaturated fatty acids
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They have saturated fatty acids
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They use unsaturated fatty acids to increase the melting temperature of the cell membrane.
Question 16
Question
The closer to 1, the purer the water solution.
Question 17
Question
Why do we use salt and sugar in food preservation?
Question 18
Question
We can use microbes to preserve our food by them producing acid. This method of food preservation is called?
Answer
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Fermentation
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Salting
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Sugaring
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Acidification
Question 19
Question
Canned fruits naturally have higher acid than canned meat or vegetables as they have naturally occurring acidity prevents the growth of this bacteria.
Answer
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E. coli
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C. botulinum
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Listeria
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Anaerobes
Question 20
Question
What is pasteurization?
Answer
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Heating food at a HIGH temperature for a LONG time to kill bacteria without destroying the nutritional value or dramatically altering the flavor of the food
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Heating food at a LOW temperature for a SHORT time to kill bacteria without destroying the nutritional value or dramatically altering the flavor of the food
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Heating food at a HIGH temperature for a SHORT time to kill bacteria without destroying the nutritional value or dramatically altering the flavor of the food
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Heating food at a LOW temperature for a LONG time to kill bacteria without destroying the nutritional value or dramatically altering the flavor of the food
Question 21
Question
What is true about anaerobes?
Answer
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Boiling water to remove gas is needed to make anaerobic media.
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Anaerobes need air to survive
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Some obligate anaerobes have superoxide reductase
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Obligate anaerobes can thrive in the presence of oxygen
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They cannot utilize oxygen as an electron acceptor
Question 22
Question
Which type of organism contains a protein-free manganese complex instead of superoxide dismutase?
Answer
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Aerobes
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Microaerophiles
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Aerotolerant
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Obligate anaerobes
Question 23
Question
Aerobes and facultative aerobes always have [blank_start]superoxide dismutase[blank_end] and [blank_start]catalase[blank_end].
Answer
-
superoxide dismutase
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catalase
Question 24
Question
Some aerotolerant organisms have [blank_start]protein-free manganese complexes[blank_end] instead of [blank_start]superoxide dismutase[blank_end]
Question 25
Question
Obligate anaerobes have [blank_start]superoxide[blank_end] [blank_start]reductase[blank_end] instead of [blank_start]superoxide[blank_end] [blank_start]dismutase[blank_end].
Answer
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superoxide
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reductase
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superoxide
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dismutase
Question 26
Question
Which of these organisms utilize oxygen as a terminal electron acceptor?
Answer
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Aerotolerant
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Aerobes
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Facultative aerobes
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Microaerophiles
Question 27
Question
Bacteriocidal [blank_start]kills[blank_end] the bacteria, bacteriostatic [blank_start]stops[blank_end] bacterial growth, and bacteriolytic [blank_start]destroys[blank_end] the cells.
Answer
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kills
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destroys
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breaks down
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stops
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grows
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slows
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destroys
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hides
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nurse
Question 28
Question
[blank_start]Sterilants[blank_end] destroy all microorganisms including endospores
[blank_start]Disinfects[blank_end] are used on surfaces to kill microorganisms but not necessarily endospores
[blank_start]Sanitizers[blank_end] reduce microbial numbers but do not sterilize
[blank_start]Antiseptics[blank_end] kill or inhibit microbial growth, but are non-toxic enough to be applied to living tissue
Answer
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Sterilants
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Disinfects
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Sanitizers
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Antiseptics