Quiz
Molecular Genetics
Create a free account to finish this quiz
Save your progress and see your final score.
Already have an account? Sign in
Questions in this quiz (74)
1The first step in the polymerase chain reaction (PCR) is
- ADenaturation
- BCooling
- CAnnealing
- DPrimer extension
2In a polymerase chain reaction, a synthetic sequence of nucleotides are involved in
- ADenaturing
- BHeating
- CCooling
- DCopying
- EAll of the above
3Bacteria protect themselves from viruses by fragmenting viral DNA upon entry with
- ALigases
- BEndonucleases
- CMethylases
- DVectors
- EProbes
4When "sticky ends" are paired, they can be joined by
- ARestriction Enzymes
- BpSC101
- CMethylases
- DX-gal
- EDNA ligase
5One of the most useful methods for identifying a specific gene is
- AWestern blot
- BSouthern blot
- CEastern blot
- DMagnetic resonance imaging
- EThin chromatography
6The Polymerase Chain Reaction is used to:
- Aamplify a small amount of DNA
- Bcleave bacterial plasmids
- Cseal "sticky ends"
- Didentify target plasmids
- Enone of the above
7When tryptophan is present in the medium, the transcription of tryptophan producing genes in E. coli is stopped by a helix-turn-helix regulator binding to the
- Atrp repressor
- Btrp operon
- Ctrp promoter
- Dtrp operator
- Etrp polymerase
8When tryptophan is present in the environment of E. coli, the tryptophan binds to the
- Atrp operon
- Btrp promoter
- Ctrp operator
- Dtrp repressor
- Etrp polymerase
9The operon that controls tryptophan producing genes in E. coli consists of _______________
- Aactivator
- Bregulator
- Cpromoter
- Doperator
- Erepressor
10In order for a gene to be transcribed, RNA polymerase must have access to the DNA helix and be able to bind to the genes
- Aactivator
- Bregulator
- Cpromoter
- Doperator
- Erepressor
11In the function of the lac operon in E. coli, the lac genes are transcribed in the presence of lactose because
- ARNA polymerase binds to the operator
- Bthe repressor cannot bind to the promoter
- Can isomer of lactose binds to the repressor
- DCAP does not bind to the operator
- Eof the absence of cAMP
12The role of methylation of DNA is now viewed as
- Ainterfering with DNA transcription by blocking base pairing between cytosine and guanine
- Bcomplexing with enhancers to prevent transcription
- Cprevention of mutation
- Dinsuring that genes that are turned off, stay off
- Eirrelevant to gene transcription
13E. coli is able to use foods other than glucose in the absence of available glucose, because falling levels of glucose cause an increase of
- AcAMP
- BCAP
- Clactase
- Dglu operon
- EtRNA
14In the absence of glucose, E. coli can import lactose to change into glucose and galactose because CAP binds to the
- AcAMP
- BDNA
- Clac operon
- Doperator
- Erepressor
15Which is not part of the lac operon?
- Arepressor
- Bactivator protein
- Coperator
- Dpromoter
- Estructural gene
16In an operon the location of the regulatory region occurs ________ the structural genes.
- Aafter
- Bwithin
- Cbefore
17Which of the following is part of an operon?
- Astructural genes
- Boperator
- Cpromoter
- Da CAP binding site
- Eall of the above
18Transcription is initiated when RNA polymerase binds to
- Apromoter
- Binitiator
- Ccodon
- Dtranscriptor
19In eukaryotes, there are _______________ codons that specify amino acids
- A21
- B24
- C61
- D64
- E100
20In eukaryotes, the "start" codon also specifies the amino acid,
- Aphenylalanine
- Bvaline
- Cmethionine
- Daspartate
21In transcription, the nucleotide sequence CAT in DNA would specify _______________ in mRNA.
- ATAC
- BGAU
- CCAT
- DGTU
- EGUA
22The genetic code consists of groups of three nucleotides called
- Acodons
- Bintrons
- Canticodons
- Dreading frame
- Etriplets
23The function of tRNA is to
- Aprovide a site for polypeptide synthesis
- Btransport amino acids to the ribosome
- Ctravel to the ribosome to direct the assembly of polypeptides
- Dtranscribe DNA
- Etranslate DNA
24In the formation of an initiation complex, a _______________ is positioned first.
- Amet-tRNA
- Bser-tRNA
- Ctyr-rRNA
- Dmval-tRNA
- Ecyst-tRNA
25The function of mRNA is to
- Aprovide a site for polypeptide synthesis
- Btransport amino acids to the ribosome
- Ctravel to the ribosome to direct the assembly of polypeptides
- Dtranscribe DNA
- Etranslate DNA
26The process of _______________ cuts introns from the primary transcript and the final "processed" mRNA is produced.
- ARNA cleaving
- BRNA translocation
- CRNA splicing
- DRNA elongation
- ERNA releasing
27In the process of translation,
- Aa strand of mRNA is formed with nucleotide sequences complementary to those of DNA
- Bnucleotide sequences of tRNA are established
- Ca polypeptide is formed in response to the rRNA nucleotide sequence
- DrRNA is synthesized with sequences complementary to those of tRNA
- Ea polypeptide is formed as dictated by the nucleotide sequence in mRNA
28In mRNA, the series of nucleotides CCC specifies
- Aserine
- Bproline
- Calanine
- Darginine
- Estop
29A molecule of tRNA with the anticodon AAA will transport the amino acid
- Aphenylalanine
- Blysine
- Cglycine
- Dleucine
- Ealanine
30As polypeptides are formed at the ribosome, elongation continues until _______________ is exposed.
- Aa release factor
- Ban intron
- Ca nonsense codon
- Dan exon
- Epolypeptidases
31Which one of the following is not a kind of RNA?
- AmRNA
- BtRNA
- CrRNA
- DnRNA
- EAll of the above are kinds of RNA
32The direct result of transcription is
- Aa duplicate DNA molecule
- BnRNA
- Cprotein
- DmRNA
- Enone of the above
33The direct result of translation is
- Aduplicate DNA
- BmRNA
- Cprotein
- DnRNA (nuclear RNA)
- Enone of the above
34Which one of the following is not associated with transcription?
- AtRNA
- BmRNA
- CnRNA
- DrRNA
- Eall of the above
35Which one of the following is not associated with translation?
- AtRNA
- BmRNA
- CrRNA
- Danticodons
- EAll are involved with translation
36According to Chargaff's rule, the following proportion exists in DNA
- AA=G
- BC=G
- CT=G
- DA>T
- EC>G
37The actual synthesis of DNA in E. coli is the function of
- Apolymerase I
- Bprimase
- Cprimer
- Dpolymerase III
- Eligase
38Since the first nucleotides cannot be linked in a newly synthesized strand in DNA replication, ___________ is required.
- ADNA primer
- BDNA ligase
- CDNA polymerase
- DRNA primer
- Ehelicase
39The fact that some viruses use DNA to direct their heredity was demonstrated by
- Afinding radioactive sulfur from a bacteriophage in a bacterium
- Bfinding radioactive phosphorus from a bacterium in a bacteriophage
- Cfinding that radioactive phosphorus from a bacteriophage had mutated in bacterium
- Dfinding radioactive phosphorus from a bacteriophage in a bacterium
- Efinding radioactive sulfur from a bacterium in a bacteriophage
40Okazaki fragments are used to elongate
- Athe leading strand toward the replication fork
- Bthe lagging strand toward the replication fork
- Cboth strands in both directions
- Dthe leading strand away from the replication fork
- Ethe lagging strand away from the replication fork
41In nucleic acids, the free hydroxyl group is attached to the _______________ carbon of the sugar.
- A5'
- B4'
- C3'
- D2'
- E1'
42A single enzyme is specified by a single
- Agene
- Bchromosome
- Cnucleosome
- Dnucleotide
- Ehistone
43In replication of DNA, the helix is opened and untwisted by
- Aribase
- Bprimase
- Cdeoxase
- Dhelicase
- Eligase
44_______________ join DNA fragments to the lagging strand
- Atelomeres
- Bcentromeres
- Chelicases
- Dligases
- Epolymerases
45Which statement about the elongation of the lagging strand during DNA replication is correct?
- AIt is synthesized in a 3' --> 5' direction.
- BIt progresses (grows) toward the replication fork
- CIt requires a short RNA primer to proceed.
- DIt is synthesized by DNA ligase
- EIt is synthesized continuously
46If one side of a DNA molecule contains the following sequence of nucleotides, AGTCCG, the complementary sequence on the other side would be:
- AGCCTGA
- BAGTCCG
- CTCAGGC
- DCTGAAT
- Enone of the above
47During your summer job at Virotech, you isolate a previously unknown virus. Analysis of its genome reveals that it is composed of a double stranded DNA molecule containing 14% T (thymine). Based on this information, what would you predict the %C (cytosine) to be?
- A14%
- B28%
- C36%
- D72%
- Ecan't tell!
48The process of acquiring a gene from another organism is called transformation.
- ATrue
- BFalse
49The Hershey-Chase experiment showed that the virus protein is responsible for directing the production of new viruses.
- ATrue
- BFalse
50The two sides of the double helix structure of DNA are held together with hydrogen bonds.
- ATrue
- BFalse
51DNA replication is called semiconservative because half of each of the two new strands of DNA is "old" DNA from the original DNA molecule and half is "new" DNA.
- ATrue
- BFalse
52Genetic engineering has been used to do all of the following except:
- Amake plants more resistant to frost
- Bmake plants more resistant to disease
- Cmake plants more resistant to herbicides
- Dimprove the nutritional balance of plants
- Eall of the above
53According to Mendel, which of the following cause an organism's size, colour and markings?
- AChromosomes
- BGenes
- CAlleles
- DFactors
54Which term is associated with eukaryotic DNA?
- Achrosome
- Bhistone
- Ctelomere
- Dall of the above
55If 28% of a segment of DNA is composed of guanine, how much of the segment is adenine?
- A7%
- B22%
- C28%
- DThere is no way to determine this.
56How did the work of Franklin and Wilkins contribute to the discovery of the structure of DNA?
- AThey determined the rule of base pairing
- BThey determined that DNA has an X shape
- CThey determined the double helix structure
- DThey determined that the strands run antiparallel
57Which of these findings made Watson and Crick confident that their DNA model was correct?
- AChargaff's base-pairing ratios
- BPauling's three-strand model
- CFranklin's early disagreement with the double helix theory
- DAll of the above
58What would have been the results of Meselson and Stahl's experiment if DNA had undergone conservative replication?
- Aone band containing only 14N
- Bone band containing only 15N
- Ctwo bands: one of 15N and 14N
- Dthree bands: one of 15N, one of 14N, and one of 1:1 14N:15N
59Which characteristics of the structure of DNA results in a lagging strand and a leading strand during DNA replication?
- Aits phosphate backbone
- Bits antiparallel strands
- Cthe ratio of purines to pyrimidines
- Dthe full twist every ten nucleotides
60What is the function of DNA ligase in replication?
- AIt initiates a replication bubble
- BIt prevents DNA strands from tangling
- CIt places nucleotides to form a new DNA strand
- DIt fills gaps on the lagging strand
61Which method do prokaryotes use to compact DNA?
- ASpooling around a histone
- BForming a nucleosome
- CSupercoiling
- DWinding into solenoids
62Where are telomeres located?
- AAt ends of chromosomes
- BOn the leading strand
- CAt the replication fork
- DOn prokaryotic plasmids
63What is the name of Beadle and Tatum's original hypothesis?
- AOne gene-one sequence hypothesis
- BOne gene-one enzyme hypothesis
- COne gene-one peptide hypothesis
- DOne gene-one polypeptide hypothesis
64What is the sequence of information transfer, as outlined by the central dogma?
- ADNA -> tRNA -> mRNA --> polypeptide
- BDNA -> mRNA -> tRNA --> polypeptide
- CDNA -> mRNA -> rRNA --> polypeptide
- DDNA -> rRNA -> tRNA --> polypeptide
65Where is the TATA box found?
- AIn the termination sequence that stops DNA polymerase
- BIn the termination sequence that stops RNA polymerase
- CIn the promoter that enables the binding of DNA polymerase
- DIn the promoter that enables the binding of RNA polymerase
66What happens during capping and tailing?
- AA methyl 5' tail and poly(A) cap are added
- BA methyl 3' tail and poly(A) cap are added
- CA methyl 5' cap and poly(A) tail are added
- DA methyl 3' cap and poly(A) tail are added
67How is the lac operon inhibited?
- AThe lac repressor is not synthesized by the lacl gene
- BRNA polymerase is inhibited by high concentrations of allolactase
- CThe lac repressor is inhbited by high concentrations of allolactase
- DAllolactase is not present to bind with the lac repressor
68Which term refers to a point mutation that results in a different amino acid in a particular position?
- AMissense mutation
- BSilent mutation
- CNonsense mutation
- DFrameshift mutation
69What is the molecular tool scientists use to cut a DNA fragment from a larger piece of DNA?
- ARestriction enzymes
- BDNA primase
- CMethylase
- DDNA ligase
70After excising a DNA fragment that contains a target gene, what should a molecular biologist do?
- AIntroduce a vector into a suitable organism
- BIsolate a desired gene or DNA fragment
- CSelect the transformed cells
- DInsert the gene into a vector
71Which chemical compound can be used to stain a completed electrophoresis gel?
- AEthidium bromide
- BLoading dye
- CMolecular marker
- DDNA polymerase
72During PCR, double-stranded DNA is separated into two single strands of DNA. What is this step called?
- ASplicing
- BDenaturation
- CExtension
- DAnnealing
73After 8 PCR cycles, how many target sequences have been synthesized?
- A8
- B16
- C240
- D256
74How does genetic therapy differ from traditional methods of treating a disease?
- AIt targets the effects of the disease
- BIn some cases, it fixes the genetic code itself
- CIt reverses the damage done to the body
- DIt replaces the need for organ transplant
Information
- Description
- Quiz on Molecular Genetics, created by Nadia Siddique on 17/01/2016.
- Author
- Nadia Siddique
- Language
- English
- Views
- 99
- Tags
- Rating
- No ratings yet Rate this quiz