7779684
Description
Quiz by Daniel Brand, updated more than 1 year ago
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Created by Daniel Brand
almost 8 years ago
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Question 1
Question
Which of the following statements are true about chromatography? Select all that apply.
Answer
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It separates mixtures into its distinct constituents.
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It consists of stationary and mobile phases.
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The solvent used is water-based.
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Chromatography can only separate molecules based on their size.
Question 2
Question
TLC stands for [blank_start]thin[blank_end] [blank_start]layer[blank_end] [blank_start]chromatography[blank_end].
Answer
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thin
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layer
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chromatography
Question 3
Question
For which of the following biological molecules would you use water as the mobile phase? Check all that apply.
Answer
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Amino acids
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Fats
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Glucose
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Oils
Question 4
Answer
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Beaker
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Stationary phase
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Mobile phase
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Watch glass
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Chromatography paper
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Solvent
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Spot of mixture
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Beaker
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Pencil line
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Watch glass
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Stationary phase
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Beaker
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Pencil line
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Solvent
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Solvent
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Stationary phase
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Spot of mixture
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Chromatography paper
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Watch glass
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Solvent
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Pencil line
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Beaker
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Chromatography paper
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Spot of mixture
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Spot of mixture
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Chromatography paper
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Beaker
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Watch glass
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Stationary phase
Question 5
Question
The rate at which different molecules move along the stationary phase depends on...
Answer
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Size
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Polarity
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Solubility
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Electronic charge
Question 6
Question
Which of the following statements best explains how different molecules 'climb' chromatography paper at different rates?
Answer
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Polar molecules travel faster because they don't form hydrogen bonds with the paper.
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Polar molecules travel faster because they form hydrogen bonds with the paper.
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Non-polar molecules travel faster because they don't form hydrogen bonds with the paper.
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Non-polar molecules travel faster because they form hydrogen bonds with the paper.
Question 7
Question
Once a chromatography run has completed, you can identify the components in the mixture by finding their Rf values.
Rf = x / y
Which statements are true about this formula?
Answer
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x is the distance travelled by the solvent
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x is the distance travelled by the component
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y is the distance travelled by the solvent
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y is the distance travelled by the component
Question 8
Question
Three ways of visualising the molecular components separated during chromatography include shining [blank_start]a UV light[blank_end] over a [blank_start]TLC plate[blank_end], spraying [blank_start]ninhydrin[blank_end] on [blank_start]amino acid molecules[blank_end], or adding [blank_start]iodine crystals[blank_end].
Answer
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a UV light
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white light
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different coloured lights
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infrared light
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TLC plate
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gas chromatography column
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mobile phase
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ninhydrin
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amino acid molecules
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starch
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sodium hydrogen carbonate
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amino acid molecules
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triglyceride molecules
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reducing sugars
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non-reducing sugars
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iodine crystals
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chlorine gas
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alkaline solution
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copper sulphate
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