L39 Microbial growth and behaviour (Winogradsky column)

Description

1.Describe the different energy and carbon sources used by microorganisms. 2.Describe the growth characteristics of prokaryotes in a ‘closed’ batch culture system. 3.Outline some examples of microbial behaviour.
Mer Scott
Quiz by Mer Scott, updated more than 1 year ago
Mer Scott
Created by Mer Scott about 7 years ago
109
2

Resource summary

Question 1

Question
Choose the incorrect statement about trophic nourishment.
Answer
  • Chemoheterotrophs can get their energy and carbon from the same organic compound.
  • A phototroph uses light as en energy source, and can be oxygenic or anoxygenic.
  • A chemotroph uses chemical energy as an energy source.
  • Autotrophs can make complex carbons from CO2.
  • Anoxygenic phototrophs oxidise molecules like H2S and do not produce oxygen.
  • Oxygenic phototrophs reduce H2O and produce oxygen.

Question 2

Question
Choose the correct statements about cyanobacteria.
Answer
  • They use light as an energy source.
  • They oxidise water for electrons.
  • They use complex carbons for a carbon source.
  • They carry out oxygenic photosynthesis.
  • They reduce water for electrons.

Question 3

Question
Choose the incorrect statement about green and purple cyanobacteria.
Answer
  • They are photoautotrophs.
  • They oxidise H2S to S for an electron source.
  • They use CO2 for an energy source.

Question 4

Question
Choose the correct statement about colourless sulfur bacteria(Beggiatoa).
Answer
  • They sit below green and purple sulfur bacteria in the Winogradsky column.
  • They are chemoheterotrophs.
  • They oxidise H2S to SO4.
  • They use complex organic carbons for a carbon source.

Question 5

Question
Sulfate reducing bacteria are chemoheterotrophs that essentially reverse the processes that colourless sulfur bacteria carry out.
Answer
  • True
  • False

Question 6

Question
Organisms which carry out fermentation are [blank_start]chemoheterotrophs[blank_end]. Their energy source, carbon source, and electron donor, are always [blank_start]complex carbons[blank_end] (organic molecules) and sometimes are even the same molecule. An example of [blank_start]a fermenting chemoheterotroph[blank_end] are cellulose degrading bacteria, which break pyruvate down into [blank_start]acetate[blank_end] and simultaneously degrade cellulose into [blank_start]lactate[blank_end]. Other examples include clostridium and [blank_start]rumen[blank_end] bacteria.
Answer
  • chemoheterotrophs
  • chemoautotrophs
  • complex carbons
  • fermenting chemoheterotrophs
  • fermenting chemoautotrophs
  • acetate
  • acetic acid
  • lactate
  • lactose
  • rumen
  • colourless sulfur

Question 7

Question
Microbial growth in a [blank_start]closed batch[blank_end] culture system is not like nature, in that it is a 'Feast and [blank_start]Famine[blank_end]' situation controlled by the examiner. There are four stages which characterise the growth [blank_start]curve[blank_end] for bacteria in this system. Firstly, there is a [blank_start]lag phase[blank_end]. It is shown as little to no change in the population size. The lag period [blank_start]differs depending[blank_end] on how much the inoculum must evolve and adjust before it is suited to the environment/has the proper [blank_start]biosynthetic reactions[blank_end] taking place. The next phase is the [blank_start]exponential phase[blank_end]. During this phase there are [blank_start]no limited resources[blank_end] or competition and so the bacterial population can [blank_start]double[blank_end] in size every generation via binary fission, displaying exponential population growth. The next phase is the [blank_start]stationary phase[blank_end]. This appears to be a stable population size, but does not indicate static activity. Instead, it reflects the cryptic growth of the organisms. Cryptic growth is the state of [blank_start]dynamic[blank_end] equilibrium where organisms survive by feeding from the lysed, dead bodies of deceased organisms. Finally, there will be a [blank_start]death phase[blank_end] where the population size decreases until it is at a stable end point.
Answer
  • closed batch
  • open batch
  • Famine
  • curve
  • lag phase
  • exponential phase
  • stationary phase
  • death phase
  • depends
  • biosynthetic reactions
  • double
  • no limited resources
  • limited resources
  • dynamic

Question 8

Question
What might cause a persistent bacterial infection?
Answer
  • Microbes which are able to degrade antibiotics.
  • Microbes not all being in the same stage of a life cycle, and the non-dividing organisms not being targeted by antibiotics.
  • Microbes which can repopulate faster than a patient can be medicated.

Question 9

Question
To be antibiotic resistant a microbe must carry the genes which confer resistance.
Answer
  • True
  • False

Question 10

Question
Choose the incorrect statement about microbial behaviour.
Answer
  • Some bacteria are very large e.g. thiomargarita.
  • Some bacteria have organelles e.g. Anammox bacteria
  • Myxobacteria display social behaviour.
  • None of these are incorrect.

Question 11

Question
Which statement best describe the 'sliding' behaviour displayed by myxobacteria.
Answer
  • It is a form of social motility wherein each bacteria pulls with pili and secretes slime like a propellent.
  • It is the microbial version of hunting like a wolf pack.
  • It is swarm feeding cooperatively by sharing enzymes and attacking targets as a group.

Question 12

Question
When a food source is exhausted, myxobacteria form 3D structures of endospores. What are these referred to as?
Answer
  • Fruiting bodies
  • Sporing bodies
  • Endospikes
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