1496722
Beschreibung
Mindmap von Olivia Farrow, aktualisiert more than 1 year ago
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Erstellt von Olivia Farrow
vor etwa 10 Jahre
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Cell Fractionation
- Requirements of the solution?
- Cold- Reduce enzyme activity
that might break down the
ogranelles
- Isotonic- Prevents
organelles bursting or
shrinking as a result of
osmotic gain or loss of
water.
- Has the same water potential as
the original tissue.
- Has the same water potential as
the original tissue.
- Buffered-
Maintain a
constant pH.
- Cold- Reduce enzyme activity
that might break down the
ogranelles
- Homogenation
- Cells are broken up by a
homogeniser to release the
organelles from the cell.
- The resultant fluid
is known as the
homogenate
- This is filtered
to remove and
complete cells
and large
pieces of debris
- This is filtered
to remove and
complete cells
and large
pieces of debris
- Cells are broken up by a
homogeniser to release the
organelles from the cell.
- Process
where cells
are broken up
and the
different
organelles
separated out
- The techniques of cell fractionation and
ultracentrifugation enables considerable
advances in biological knowledge. They
allowed a detailed study of the structure
and function of organelles.
- The techniques of cell fractionation and
ultracentrifugation enables considerable
advances in biological knowledge. They
allowed a detailed study of the structure
and function of organelles.
- ULTRACENTRIFUGATION
- Process in which
the homogenate is
spun at a high
speed to create a
centrifugal force.
- 1) Tube of filtrate is spun at low speed.
- 2) Heaviest organelles, the nuclei, are
forced to the bottom to form a thin
sediment pellet.
- 3) Supernatant is removed, leaving the sediment of nuclei.
- 4) Supernatant is transferred to another tube, and
spun in the centrifuge at a faster speed.
- ~PROCESS IS REPEATED~
- ~PROCESS IS REPEATED~
- 4) Supernatant is transferred to another tube, and
spun in the centrifuge at a faster speed.
- 3) Supernatant is removed, leaving the sediment of nuclei.
- 2) Heaviest organelles, the nuclei, are
forced to the bottom to form a thin
sediment pellet.
- 1) Tube of filtrate is spun at low speed.
- Process in which
the homogenate is
spun at a high
speed to create a
centrifugal force.
- Order of separation of organelles
(and speed of centrifugation)
- Nuclei- 1 000
- Mitochondria- 3 500
- Lysosomes- 16 500
- Ribosomes- 100 000
- Ribosomes- 100 000
- Lysosomes- 16 500
- Mitochondria- 3 500
- Nuclei- 1 000
Medienanhänge
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