Enzyme Kinetics

Michaelis Constant (Km)
1. [Substrate] when rxn rate = 1/2 Vmax
  1. Indicates [Substrate] needed to speed up rxn
    1. High [substrate] means low affinity for substrate
      1. Km inversely proportional to enzyme-substrate affinity
Vmax
1. Maximum rxn rate
Saturation Kinetics
1. [Substrate] increase, rate of rxn increases until Vmax is achieved
Cofactor
1. Non-protein component required by some enzymes to reach optimal activity
  1. Can be coenzymes or metal ions
    1. Coenzymes
      1. Organic molecules
        Water-soluble vitamins
        Cosubstrates
        Reversibly bind to an enzyme, transfer a chemical group to another substrate
        Reverts to original form via other enzymatic rxn
        Example: ATP
        Prosthetic groups
        Binds covalently to enzyme during rxn
        Emerges from rxn unchanged
        Example: Heme
        Heme binds to Catalase in peroxisomes to degrade H2O2
    2. Metal Ions

Zusammenfassung der Ressource

	Erstellt von Christopher Boga vor fast 9 Jahre