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Beschreibung
Quiz von Christina Marshall, aktualisiert more than 1 year ago
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Erstellt von Christina Marshall
vor fast 7 Jahre
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Frage 1
Frage
Vectors used to clone genes were derived originally from
Antworten
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proteins
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plasmids
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viruses
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all of the above
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b and c only
Frage 2
Frage
Restriction enzymes used in most cloning experiments
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cut CNA into pieces for gene cloning
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are naturally produced by bacteria cells to prevent viral infection
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produce sticky ends on DNA fragments
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All of the above are correct
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only a and c are correct
Frage 3
Frage
DNA ligase is needed in a cloning experiment
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to promote hydrogen bonding between sticky ends
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to catalyze the covalent attachment of the backbone of DNA strands
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to digest the chromosomal DNA into small pieces
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Only a and b are correct
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a, b, and c are correct
Frage 4
Frage
Let's suppose you followed protocols described in figures 20.2 and 20.3. Which experiment(s) would you conduct to confirm that a white colony really contained a recombinant vector with an inserted fragment of DNA?
Antworten
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Pick a bacterial colony and restreak on plates containing X-Gal to confirm that the cells really form white colonies.
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Pick a bacterial colony, isolate plasmid DNA, digest the plasmid DNA with a restriction enzyme, and then preform gel electrophoresis with the DNA.
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Pick a bacterial colony and test it to see it X-Gal is functional within the bacterial cells.
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Pick a bacterial colony and retest it on ampicillin-containing plates to double-check that the cells are really ampicillin resistant.
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Both c and d should be conducted.
Frage 5
Frage
Why is Taq polymerase used in PCR rather than other DNA polymerases?
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Taq polymerase is a synthetic enzyme that produces DNA strands at a faster rate than natural polymerases.
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Taq polymerase is a heat-stable form of DNA polymerase that can function after exposure to the high temperatures necessary for PCR.
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Taq polymerase is easier to isolate than other DNA polymerases.
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Taq polymerase is the DNA polymerase commonly produced by most eukaryotic cells.
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All of the above are correct.
Frage 6
Frage
Let's suppose you want to clone a gene that has never been analyzed before by DNA sequencing. Which of the following statements do you agree with the most?
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Do PCR to clone the gene because it is much faster.
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Do PCR to clone the gene because it is very specific and gives a high yield.
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You can't do PCR because you can't make forward and reverse primers.
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Do cloning by insertion into a vector because it will give you a higher yield.
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Do cloning by insertion into a vector because it is easier than PCR.
Frage 7
Frage
The method of determining the base sequence of DNA is termed
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PCR
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gene cloning
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DNA fingerprinting
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DNA sequencing
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gene mapping
Frage 8
Frage
With regard to dideoxy sequencing, which of the following statements is false?
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The dideoxy nucleotides are fluorescently labeled.
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The dideoxy nucleotides cannot be incorporated into a growing DNA strand.
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When incorporated into a DNA strand, the dideoxynucleotides prevent further growth of the strand.
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The dideoxy sequencing method is used to determine the base sequence of DNA.
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The dideoxy sequencing method requires the use of primers.
Frage 9
Frage
Organisms that carry genes that were introduced using molecular techniques are called
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transgenics
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recombinant DNA
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mutants
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genetically modified organisms
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both a and d
Frage 10
Frage
DNA fingerprinting is used
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to precisely identify an organism, such as the identification of specific strains of bacteria
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as a forensics tool to provide evidence in a criminal case
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to determine the genetic relationship between individuals
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to determine the identity of an individual
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for all of the above.
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