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Viruses contain either DNA OR RNA
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A virus that is composed not of DNA but of RNA is called a [blank_start]retrovirus[blank_end]
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Viroids are infectious agents without protein
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Prions –are infectious agents with both DNA and RNA
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Adenine and guanine are
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A nucleoside is formed when a
base is linked to the WHAT carbon of a
deoxyribose (or ribose) molecule
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dCMP and dAMP are examples of [blank_start]nucleotides[blank_end]
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nucleotides
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nucleosides
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proteins
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nucleic acid
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In DNA and RNA, nucleotides are linked by phosphodiester bonds between the [blank_start]5′[blank_end] and 3′ carbons
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In all prokaryotes and eukaryotes DNA is double-stranded
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Guanine and Cytosine form a base-pair held together by [blank_start]3[blank_end] hydrogen bonds.
Adenine and Thymine form a base-pair held together by [blank_start]2[blank_end] hydrogen bonds.
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Most or all cellular DNA is A-DNA
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Z-DNA is left handed and A-DNA is right handed
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Underwinding generates [blank_start]negative[blank_end] supercoils
Overwinding generates [blank_start]positive[blank_end] supercoils
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Single stranded DNA absorbs more UV than double
stranded DNA
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UV absorbance rises as DNA
denatures is known as the [blank_start]hyperchromic shift[blank_end]
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Tm (The temperature needed to denature
50% of the DNA molecules in a sample) is increased in DNA with high content of AT base-pairs
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What are three key structural differences between
DNA and RNA?
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RNA has ribose instead of deoxyribose.
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RNA has deoxyribose instead of ribose
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RNA contains adenine, cytosine and guanine (like DNA) but has Uracil (U) instead of thymine.
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RNA contains adenine, cytosine and guanine (like DNA) but has thymine (T) instead of uracil
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RNA is usually double stranded
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RNA is usually single stranded
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Plasmids carry non-essential genes, e.g. for
antibiotic resistance
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Bacterial genomes usually consist of a single, linear chromosome made of double-stranded DNA
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Heterochromatin
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Euchromatin
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(Length of DNA/ Length of structure DNA packed into) is known as the [blank_start]packing ratio[blank_end]
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By mass, the composition of a chromosome at mitosis is
approximately
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1/2 DNA and 1/2 histone proteins
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1/3 DNA and 2/3 histone proteins
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1/3 DNA, 1/3 histone proteins and 1/3 non histone proteins
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1/2 DNA. 1/4 histone proteins and 1/4 non histone proteins
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20% of amino acids are arginine or lysine
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[blank_start]Condensin[blank_end] is a ring-shaped protein that can anchor the ends of a loop of DNA
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[blank_start]topoisomerase II[blank_end] is an enzyme that can remove supercoils from DNA
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Acetylation removes positive charge from side-chain and so [blank_start]weakens[blank_end]
interaction between histones and negatively-charged DNA
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Eukaryotic chromosomes have 1 origin of replication
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In bacterial replication, replication begins at a single AT-rich origin and is
bidirectional
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What enzyme carries out transcriptase?
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Maltase
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DNA transcriptase
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RNA
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RNA polymerase
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DNA helicase
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To synthesise RNA, RNA polymerases require:
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RNA polymerase binds to the promoter in the [blank_start]initiation[blank_end] stage
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RNA polymerase dissociates from DNA releasing
the new RNA molecule in the [blank_start]transcriptase[blank_end] stage
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RNA polymerase moves along the DNA molecule
synthesising an RNA copy in the [blank_start]elongation[blank_end] stage
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A [blank_start]consensus[blank_end] sequence is a sequence of DNA having similar structure and function in different organisms.
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The holoenzyme carries out elongation but not initiation
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Eukaryotic promoters differ from those in bacteria
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RNA polymerase [blank_start]I[blank_end] - synthesises rRNA
RNA polymerase [blank_start]III[blank_end] - synthesises tRNA
RNA polymerase [blank_start]II[blank_end] - synthesises mRNA
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I
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ll
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lll
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I
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ll
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lll
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I
-
ll
-
lll
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RNA polymerase II produces a 1° transcript (aka pre-mRNA)
which is processed in the nucleus to form mRNA. The main processing reactions are
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Promoters recognised by RNA polymerase II usually consists of a core promoter that may include a [blank_start]TATA[blank_end] box
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RNA polymerases and DNA polymerases share the same mechanism.
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By mass, ribosomes have about a 1:2 ratio of rRNA to protein
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The size of ribosomes is measured in Svedbergs (S)
- sedimentation rate during centrifugation.
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The small subunit on a ribosome catalyses peptide bond formation, the
large subunit binds the mRNA being translated
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The small (30S) ribosome subunit binds to the ribosome
binding site on the mRNA in E.Coli
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There are several ribosome binding sites on mRNA in eukaryotes
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A sequence has [blank_start]three[blank_end] possible reading frames depending
on where translation starts from
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The region between an initiation codon (AUG) and a
termination codon is an“open-reading frame”
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How do amino acids recognise codons?
Adaptor molecules deliver amino acids to the appropriate codons. These adaptors are transfer RNAs
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How long are tRNAs?
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Large: 220-293 nucleotides long
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Small: 50-73 nucleotides long
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Small: 73-90 nucleotides long
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Large: 320-393 nucleotides long
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All tRNAs have an anticodon
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All tRNAs have the sequence GCA at their 3' end.
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the ability of some bases at 5' end of anticodon to pair with more than one base at 3' end of codon is known as the [blank_start]base wobble[blank_end]
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The enzymes that link tRNAs to amino acids are called
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aminoakyl-tRNA synthetases.
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aminoacyl-tRNA synthetases.
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aminoacyl-rRNA synthetases.
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aminoakyl-rRNA synthetases.
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The classic example of inducible genes are the three genes required for utilisation of lactose by E. coli. These are:
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Lac Z gene
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Lac B gene
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Lac A gene
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Lac Y gene
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Lac T gene
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The [blank_start]operator[blank_end] is the binding
site for lac repressor
protein.
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The Lac [blank_start]i[blank_end] gene encodes the
Lac repressor protein.
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Transcription of the genes of the lac operon is repressed by the lac [blank_start]repressor[blank_end] protein
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When lactose is absent, the lac repressor protein binds to the promoter
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When the lac repressor protein is bound to the operator,
RNA polymerase can still bind to the promoter but cannot
initiate transcription.
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When lactose is present some is
converted into an isomer called
[blank_start]allolactose[blank_end].
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Allolactose binds to the lac repressor protein inducing a conformational change so that lac repressor cannot bind to operator.
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Lactose breakdown is only necessary if glucose is not available. Therefore when both lactose and glucose are present in environment, lac operon genes only transcribed weakly. This effect is called [blank_start]Catabolite[blank_end] Repression
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Removal of catabolite repression requires:
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When glucose conc’ is high the cAMP conc’ is low
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[blank_start]Monosaccharides[blank_end] are colourless, crystalline solids, water soluble and most are sweet tasting.
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Monosaccharides
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Polysaccharides
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Oligosaccharides
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In general, a molecule with n chiral centres can have [blank_start]2[blank_end]n stereoisomers
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[blank_start]Epimers[blank_end]: each of two isomers with different configurations of atoms about one of several asymmetric carbon atoms present