Biochemistry undergrad quiz (still making)

Beschreibung

Biochemistry Quiz am Biochemistry undergrad quiz (still making), erstellt von Lily O am 14/12/2019.
Lily O
Quiz von Lily O, aktualisiert more than 1 year ago
Lily O
Erstellt von Lily O vor mehr als 4 Jahre
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Zusammenfassung der Ressource

Frage 1

Frage
Viruses contain either DNA OR RNA
Antworten
  • True
  • False

Frage 2

Frage
A virus that is composed not of DNA but of RNA is called a [blank_start]retrovirus[blank_end]
Antworten
  • retrovirus

Frage 3

Frage
Viroids are infectious agents without protein
Antworten
  • True
  • False

Frage 4

Frage
Prions –are infectious agents with both DNA and RNA
Antworten
  • True
  • False

Frage 5

Frage
Adenine and guanine are
Antworten
  • pyrimidines
  • purines

Frage 6

Frage
A nucleoside is formed when a base is linked to the WHAT carbon of a deoxyribose (or ribose) molecule
Antworten
  • 1'
  • 2'
  • 3'
  • 4'

Frage 7

Frage
dCMP and dAMP are examples of [blank_start]nucleotides[blank_end]
Antworten
  • nucleotides
  • nucleosides
  • proteins
  • nucleic acid

Frage 8

Frage
In DNA and RNA, nucleotides are linked by phosphodiester bonds between the [blank_start]5′[blank_end] and 3′ carbons
Antworten
  • 2'
  • 3'
  • 4'
  • 5'

Frage 9

Frage
In all prokaryotes and eukaryotes DNA is double-stranded
Antworten
  • True
  • False

Frage 10

Frage
Guanine and Cytosine form a base-pair held together by [blank_start]3[blank_end] hydrogen bonds. Adenine and Thymine form a base-pair held together by [blank_start]2[blank_end] hydrogen bonds.
Antworten
  • 3
  • 2

Frage 11

Frage
Most or all cellular DNA is A-DNA
Antworten
  • True
  • False

Frage 12

Frage
Z-DNA is left handed and A-DNA is right handed
Antworten
  • True
  • False

Frage 13

Frage
Underwinding generates [blank_start]negative[blank_end] supercoils Overwinding generates [blank_start]positive[blank_end] supercoils
Antworten
  • negative
  • positive

Frage 14

Frage
Single stranded DNA absorbs more UV than double stranded DNA
Antworten
  • True
  • False

Frage 15

Frage
UV absorbance rises as DNA denatures is known as the [blank_start]hyperchromic shift[blank_end]
Antworten
  • hyperchromic shift

Frage 16

Frage
Tm (The temperature needed to denature 50% of the DNA molecules in a sample) is increased in DNA with high content of AT base-pairs
Antworten
  • True
  • False

Frage 17

Frage
What are three key structural differences between DNA and RNA?
Antworten
  • RNA has ribose instead of deoxyribose.
  • RNA has deoxyribose instead of ribose
  • RNA contains adenine, cytosine and guanine (like DNA) but has Uracil (U) instead of thymine.
  • RNA contains adenine, cytosine and guanine (like DNA) but has thymine (T) instead of uracil
  • RNA is usually double stranded
  • RNA is usually single stranded

Frage 18

Frage
Plasmids carry non-essential genes, e.g. for antibiotic resistance
Antworten
  • True
  • False

Frage 19

Frage
Bacterial genomes usually consist of a single, linear chromosome made of double-stranded DNA
Antworten
  • True
  • False

Frage 20

Antworten
  • Heterochromatin
  • Euchromatin

Frage 21

Frage
(Length of DNA/ Length of structure DNA packed into) is known as the [blank_start]packing ratio[blank_end]
Antworten
  • packing ratio

Frage 22

Frage
By mass, the composition of a chromosome at mitosis is approximately
Antworten
  • 1/2 DNA and 1/2 histone proteins
  • 1/3 DNA and 2/3 histone proteins
  • 1/3 DNA, 1/3 histone proteins and 1/3 non histone proteins
  • 1/2 DNA. 1/4 histone proteins and 1/4 non histone proteins

Frage 23

Frage
20% of amino acids are arginine or lysine
Antworten
  • True
  • False

Frage 24

Frage
[blank_start]Condensin[blank_end] is a ring-shaped protein that can anchor the ends of a loop of DNA
Antworten
  • Condensin

Frage 25

Frage
[blank_start]topoisomerase II[blank_end] is an enzyme that can remove supercoils from DNA
Antworten
  • topoisomerase II

Frage 26

Frage
Acetylation removes positive charge from side-chain and so [blank_start]weakens[blank_end] interaction between histones and negatively-charged DNA
Antworten
  • strengthens
  • weakens

Frage 27

Frage
Eukaryotic chromosomes have 1 origin of replication
Antworten
  • True
  • False

Frage 28

Frage
In bacterial replication, replication begins at a single AT-rich origin and is bidirectional
Antworten
  • True
  • False

Frage 29

Frage
What enzyme carries out transcriptase?
Antworten
  • Maltase
  • DNA transcriptase
  • RNA
  • RNA polymerase
  • DNA helicase

Frage 30

Frage
To synthesise RNA, RNA polymerases require:
Antworten
  • A primer
  • A double stranded template DNA molecule that Includes a promoter sequence
  • All four nucleoside triphosphates

Frage 31

Frage
RNA polymerase binds to the promoter in the [blank_start]initiation[blank_end] stage
Antworten
  • initiation

Frage 32

Frage
RNA polymerase dissociates from DNA releasing the new RNA molecule in the [blank_start]transcriptase[blank_end] stage
Antworten
  • transcriptase

Frage 33

Frage
RNA polymerase moves along the DNA molecule synthesising an RNA copy in the [blank_start]elongation[blank_end] stage
Antworten
  • elongation

Frage 34

Frage
A [blank_start]consensus[blank_end] sequence is a sequence of DNA having similar structure and function in different organisms.
Antworten
  • consensus

Frage 35

Frage
The holoenzyme carries out elongation but not initiation
Antworten
  • True
  • False

Frage 36

Frage
Eukaryotic promoters differ from those in bacteria
Antworten
  • True
  • False

Frage 37

Frage
RNA polymerase [blank_start]I[blank_end] - synthesises rRNA RNA polymerase [blank_start]III[blank_end] - synthesises tRNA RNA polymerase [blank_start]II[blank_end] - synthesises mRNA
Antworten
  • I
  • ll
  • lll
  • I
  • ll
  • lll
  • I
  • ll
  • lll

Frage 38

Frage
RNA polymerase II produces a 1° transcript (aka pre-mRNA) which is processed in the nucleus to form mRNA. The main processing reactions are
Antworten
  • Initiation
  • RNA splicing
  • DNA splicing
  • Cleavage and polyadenylation
  • Capping
  • Druining

Frage 39

Frage
Promoters recognised by RNA polymerase II usually consists of a core promoter that may include a [blank_start]TATA[blank_end] box
Antworten
  • TATA
  • TAAT
  • ATAT
  • ATTA

Frage 40

Frage
RNA polymerases and DNA polymerases share the same mechanism.
Antworten
  • True
  • False

Frage 41

Frage
By mass, ribosomes have about a 1:2 ratio of rRNA to protein
Antworten
  • True
  • False

Frage 42

Frage
The size of ribosomes is measured in Svedbergs (S) - sedimentation rate during centrifugation.
Antworten
  • True
  • False

Frage 43

Frage
The small subunit on a ribosome catalyses peptide bond formation, the large subunit binds the mRNA being translated
Antworten
  • True
  • False

Frage 44

Frage
mRNA is read from
Antworten
  • 5' to 3'
  • 3' to 5'

Frage 45

Frage
The small (30S) ribosome subunit binds to the ribosome binding site on the mRNA in E.Coli
Antworten
  • True
  • False

Frage 46

Frage
There are several ribosome binding sites on mRNA in eukaryotes
Antworten
  • True
  • False

Frage 47

Frage
A sequence has [blank_start]three[blank_end] possible reading frames depending on where translation starts from
Antworten
  • three
  • one
  • two
  • four

Frage 48

Frage
The region between an initiation codon (AUG) and a termination codon is an“open-reading frame”
Antworten
  • True
  • False

Frage 49

Frage
How do amino acids recognise codons? Adaptor molecules deliver amino acids to the appropriate codons. These adaptors are transfer RNAs
Antworten
  • True
  • False

Frage 50

Frage
How long are tRNAs?
Antworten
  • Large: 220-293 nucleotides long
  • Small: 50-73 nucleotides long
  • Small: 73-90 nucleotides long
  • Large: 320-393 nucleotides long

Frage 51

Frage
All tRNAs have an anticodon
Antworten
  • True
  • False

Frage 52

Frage
All tRNAs have the sequence GCA at their 3' end.
Antworten
  • True
  • False

Frage 53

Frage
the ability of some bases at 5' end of anticodon to pair with more than one base at 3' end of codon is known as the [blank_start]base wobble[blank_end]
Antworten
  • base wobble

Frage 54

Frage
The enzymes that link tRNAs to amino acids are called
Antworten
  • aminoakyl-tRNA synthetases.
  • aminoacyl-tRNA synthetases.
  • aminoacyl-rRNA synthetases.
  • aminoakyl-rRNA synthetases.

Frage 55

Frage
The classic example of inducible genes are the three genes required for utilisation of lactose by E. coli. These are:
Antworten
  • Lac Z gene
  • Lac B gene
  • Lac A gene
  • Lac Y gene
  • Lac T gene

Frage 56

Frage
The [blank_start]operator[blank_end] is the binding site for lac repressor protein.
Antworten
  • operator

Frage 57

Frage
The Lac [blank_start]i[blank_end] gene encodes the Lac repressor protein.
Antworten
  • i

Frage 58

Frage
Transcription of the genes of the lac operon is repressed by the lac [blank_start]repressor[blank_end] protein
Antworten
  • repressor

Frage 59

Frage
When lactose is absent, the lac repressor protein binds to the promoter
Antworten
  • True
  • False

Frage 60

Frage
When the lac repressor protein is bound to the operator, RNA polymerase can still bind to the promoter but cannot initiate transcription.
Antworten
  • True
  • False

Frage 61

Frage
When lactose is present some is converted into an isomer called [blank_start]allolactose[blank_end].
Antworten
  • allolactose

Frage 62

Frage
Allolactose binds to the lac repressor protein inducing a conformational change so that lac repressor cannot bind to operator.
Antworten
  • True
  • False

Frage 63

Frage
Lactose breakdown is only necessary if glucose is not available. Therefore when both lactose and glucose are present in environment, lac operon genes only transcribed weakly. This effect is called [blank_start]Catabolite[blank_end] Repression
Antworten
  • Catabolite

Frage 64

Frage
Removal of catabolite repression requires:
Antworten
  • The Catabolite Activator Protein (CAP)
  • cAMP
  • ATP
  • Lac i gene

Frage 65

Frage
When glucose conc’ is high the cAMP conc’ is low
Antworten
  • True
  • False

Frage 66

Frage
[blank_start]Monosaccharides[blank_end] are colourless, crystalline solids, water soluble and most are sweet tasting.
Antworten
  • Monosaccharides
  • Polysaccharides
  • Oligosaccharides

Frage 67

Frage
In general, a molecule with n chiral centres can have [blank_start]2[blank_end]n stereoisomers
Antworten
  • 2
  • 1
  • 3
  • 4

Frage 68

Frage
[blank_start]Epimers[blank_end]: each of two isomers with different configurations of atoms about one of several asymmetric carbon atoms present
Antworten
  • Epimers
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