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What is the main role of cytochrome aa3?
Antworten
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Takes e- from ubiquinol and reduces oxygen to water
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Takes e- from ubiquinol and reduces oxygen to peroxide
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Takes e- from cytochrome c and reduces oxygen to peroxide
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Takes e- from cytochrome c and reduces oxygen to water
Frage 2
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Complex IV contains how many subunits?
Frage 3
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Complex IV doesn't transfer protons across the inner mitochondrial membrane
Frage 4
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Which ions does it contain, and how many of each?
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2 Fe (in haeme groups) and 3 Cu (2 as a Cu-Cu binuclear centre, 1 as a binuclear Fe-Cu centre)
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2 Cu (in haeme groups) and 3 Fe (2 as a Fe-Fe binuclear centre, 1 as a binuclear Fe-Cu centre)
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2 Mg (in haeme groups) and 3 Cu (2 as a Cu-Cu binuclear centre, 1 as a binuclear Fe-Mg centre)
Frage 5
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Cytochrome aa3 complex consists almost entirely of alpha helices
Frage 6
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Put the following in order
A- Cyt c
B- CuA centre
C- Cyt a
D- Cyt a3-CuB centre
E - Water
•Transfer from cyt a3-CuB centre to oxygen
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A-B-C-D-E
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A-C-D-B-E
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A-C-B-D-E
Frage 7
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How many protons are pumped by complex 4, and how many are used to make water (per Oxygen molecule)?
Frage 8
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Which conformation of Complex IV has the low affinity for protons?
Frage 9
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Protons bind to Complex IV during Conformation B
Frage 10
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What happens during conformation B that lowers the Complex IV's affinity for protons?
Frage 11
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No question, just look at it and learn it
Frage 12
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ATP synthase is found on the inner mitochondrial membrane
Frage 13
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ATP synthase is divided into two sections, F1 & F0
Which is peripheral and which is integral?
Frage 14
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Name the function of ATP synthase
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ATP synthesis
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ATP hydrolysis
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ATP translocation
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Proton translocation
Frage 15
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If ATP is high, and the proton gradient is low, what will Complex V do instead?
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Hydrolyse ATP
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Synthesise GTP
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Break itself down
Frage 16
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Protons flow through F1 to power the ATP synthesis in F0
Frage 17
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Name the subunits of F1
Frage 18
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Which of the following subunit(s) are the largest in F1?
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Alpha & Beta
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Gamma & Delta
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Beta and Gamma
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Alpha and Epsilon
Frage 19
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What is the role of the delta subunit in F1?
Frage 20
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The epsilon subunit of F0 connects the gamma to the C subunit
Frage 21
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F0 contains how many subunits?
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2- A & B
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3- A, B & C
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2- Alpha & Beta
Frage 22
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The F1 subunit is...
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8nm long
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18nm long
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12nm long
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90nm wide
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19nm wide
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9nm wide
Frage 23
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The Y subunit of F1 is made of 2 beta sheets that have been bent, but are bound at the base
Frage 24
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When the gamma subunit is in contact with a beta subunit, the ATP catalysis site is in it's _ formation
Frage 25
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A subunit contains ADP + Pi- what conformation is it in?
Frage 26
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Whilst the subunit is replacing ATP with ADP + Pi, the gamma subunit remains in plance
Frage 27
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What order do the three conformations work in?
Assume you start with the Loose formation
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Loose-Tight-Open
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Loose-Open-Tight
Frage 28
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State the characteristics of the C subunit of F0
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Small
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Large
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Hydrophobic
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Hydrophilic
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Integral
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Peripheral
Frage 29
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How many monomers make up the C subunit?
Frage 30
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The C subunit contains 3 rings of monomer
Frage 31
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Why is there a variance across species of the number of monomers in the C subunit?
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The more complex an organism, the more efficient the subunit
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The more complex an organism, the less efficient the subunit
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The larger the organism, the more subunits are required
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Mammals have 8, birds have 12, all lesser animals and prokaryotes have 14 or more
Frage 32
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More subunits require more protons to move them, so more subunits is less efficient
Frage 33
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How is the A subunit described?
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A proton half channel
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A proton channel
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A proton quarter channel
Frage 34
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Protons passing through the A subunit...
Frage 35
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Each subunit can hold 2 protons
Frage 36
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The momevent of the protons around the C subunit powers what?
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The movement of the gamma subunit
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The conformational changes of the beta subunits
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The attachment of the alpha subunits
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The rotation of the delta subunit
Frage 37
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The rotation of the F1 has been proved by attaching a microtubule filament to the F1 head and examining it under the microscope
Frage 38
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Only the gamma subunit of the F1 complex rotates
Frage 39
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How is the Alpha/Beta spheroid of F1 held in place?
Antworten
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B1 subunit
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B2 subunit
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Epsilon subunit
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Delta subunit
Frage 40
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For every _degree rotation, 1 molecule of ATP is produced
Frage 41
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The mechanism of the gamma subunit causes an affinity change for ATP/ADP + Pi, driving the cycle