Prostaglandin Synthase & Multi-Enzyme Complexes

Question	Answer
Prostoglandin Synthase	Converts Arachidonate to Prostoglandin H2
Role of Prostoglandin	Causes inflammation and modulates gastric acid levels
No of enzyme centres in Prostoglandin synthase	2
Cyclooxygenase	Arachidonic Acid + O2 --> Prostoglandin G2
Peroxidase	Prostoglandin G2 --> H20 + Prostoglandin H2
Monotopic protein	Attached to the membrane from one side
Attachment of Prostoglandin synthase	Hydrophobic alpha helices
Integral/Peripheral	Peripheral, but binds strongly enough to be classed as integral
Can be detached by...	Detergent
Location of PS	Inner membrane- as requires substrate of membrane lipid hydrolysis
Substrate of P.S	Hydrophobic
Travel of Substrate	Moves through hydrophobic channel in membrane to active site devoid of water
Binding of aspirin/ibruprofin	Serine 540
Enzyme complexes require...	A high degree of subunit communication
Why have M.E.C's?	Allow specialized function
Charge	Can prevent M.E.C's forming in the wrong conditions
Reason for Complex- Product	Product of one enzyme is so reactive/expensive it's better to pass it directly along a complex
Reason for Complex- Speed	Enzymes are brought closer together and so reactants move between active sites faster
Reason for Complex- Substrate cycling	Allows cycle to happen so heat can be released
Positive Collisional Interaction	Two molecules collide and have enough energy to react
Negative Collisional Interaction	Two molecules collide/don't react due to shape/charge/size issues

Resource summary

	Created by gina_evans0312 about 11 years ago