450891
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Quiz von gina_evans0312, aktualisiert more than 1 year ago
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Erstellt von gina_evans0312
vor fast 11 Jahre
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Frage 1
Frage
Which of the following is not a role of the DNAK/Hsp70 superfamily?
Antworten
-
Stabilise nascent polypeptides
-
Repaor heat shocked proteins
-
Preserve folding proteins
-
Protein transport
-
Protein degredation
Frage 2
Frage
Hsp70s (unlike DNAK's) do not require chaperones
Antworten
- True
- False
Frage 3
Frage
What is the role of DNAJ/Hsp40 when combined with DNAK/Hsp70
Antworten
-
ATPases
-
Kinases
-
Peptidyl propyl isomerases
Frage 4
Frage
DNAK/Hsp70 need NEF's in order to function
Antworten
- True
- False
Frage 5
Frage
Where is the Hsp70/DNAK binding domain for ATP?
Antworten
-
N-terminus
-
Core
-
C- terminus
Frage 6
Frage
Name the subdomains of the ATPase found in DNAK/Hsp70
Antworten
-
1A
-
1B
-
1C
-
2A
-
2B
-
2C
Frage 7
Frage
What increases the ATPase activity of the ATP binding domain?
Antworten
-
The binding of the substrate
-
Movement into an acidic compartment
-
DNAJ/Hsp40 binding
-
YDJ1 binding
-
SSA
Frage 8
Frage
The core substrate binding domain has an affinity for what?
Antworten
-
Neutral, hydrophobic aa
-
Acidic, polar aa
-
Basic, polar aa
Frage 9
Frage
The C-terminus of DNAK/Hsp 70 acts as a 'lid' for the protein
Antworten
- True
- False
Frage 10
Frage
When ATP is hydrolysed in DNAK/Hsp70, where does the C-terminus bind to?
Antworten
-
The N-terminus
-
The core
-
It doesn't move at all
-
Over the bound substrate
Frage 11
Frage
Without the assistance of DNAJ/Hsp40, the ATPase in DNAK/Hsp70 works too slowly for the C-terminus to trap the bound substrate
Antworten
- True
- False
Frage 12
Frage
Hsp70/DNAK's preferred binding pattern is 15 aa long
Antworten
- True
- False
Frage 13
Frage
What are the 'Flanking' residues (1-4, 9-13) of Hsp70's preferred binding site made of?
Antworten
-
Leu
-
Arg
-
Lys
-
Trp
Frage 14
Frage
The preferred residue for the -Core- of Hsp70's preferred binding site is Valine
Antworten
- True
- False
Frage 15
Frage
What other residues can make up the 'core' of the preferred binding site/
Antworten
-
Ile
-
Val
-
Phe
-
Tyr
Frage 16
Frage
How does this 'Preferred Binding Patter' for Hsp70 help direct the protein?
Antworten
-
In properly folded proteins, the hydrophobic stretch is hidden, and so only nascent/denatured proteins have the exposed residues for Hsp70 to bind to
-
In nascent/denatured proteins, these hydrophobic residues will be added by Hsp40, so it can recognise them
-
Stretches with the core residues are removed during protein folding, so only nascent polypeptides have them
Frage 17
Frage
What is the role of the J domain in a Hsp40?
Antworten
-
Contain the HPD peptide needed to boost Hsp40's ATPase
-
Gly/Phe rich linker for positioning
-
Dimerisation domain
-
Zn+ 'finger' for substrate binding
Frage 18
Frage
The role of the Gly/Phe rich linker does what?
Antworten
-
Makes DNAJ/Hsp40 flexible enough to bind to its respective protein
-
Makes DNAJ/Hsp40 a more effective ATPase
-
Allows DNAJ/Hsp40 to locate its respective DNAK/Hsp70
Frage 19
Frage
The Zinc 'Finger' is for substrate binding
Antworten
- True
- False
Frage 20
Frage
Which amino acids make up the HPD domain?
Antworten
-
Histadine
-
Proline
-
Aspartate
-
Leucine
-
Glycine
Frage 21
Frage
The Znc finger of DNAJ/Hsp40 can interact with the nascent polypeptide and bring it to the DNAK/Hsp70
Antworten
- True
- False
Frage 22
Frage
What is the sequence of the Zinc 'finger'?
Antworten
-
CXXCXGXG
-
GXXGXCXC
-
GXXCXGXC
-
CXXGXCXG
Frage 23
Frage
The C-terminus of Hsp40 is for tetramerisation
Antworten
- True
- False
Frage 24
Frage
What effect does the GEF factor binding have on the Hsp70/Hsp40 complex?
Antworten
-
ADP is replaced with ATP
-
ATP is replaced with ADP
-
Hsp40 dissociates
-
Newly folded protein binds
-
Newly folded protein dissociates
Frage 25
Frage
When the substrate & Hsp40 bind to Hsp70, the GEF factor also binds
Antworten
- True
- False
Frage 26
Frage
How is the nascent polypeptide transferred from the DNAJ site to the DNK site?
Antworten
-
The G/F domain is flexible enough to bind to the DNAK site and pull it in for transfer
-
The J domain is pulled like a thread through the ATPase, bringing the two sites close enough together for transfer
-
The dimerisation of the DNAJ pulls the two sites close together
Frage 27
Frage
In which of the following is Prefoldin found?
Antworten
-
Archea
-
Prokaryotes
-
Eukaryotes
Frage 28
Frage
Prefoldin can act as a substitute for Hsp70 if the organsim doesn't have the latter
Antworten
- True
- False
Frage 29
Frage
Prefoldin is ATP dependent
Antworten
- True
- False
Frage 30
Frage
Prefolding is a...
Antworten
-
Dimer
-
Tetramer
-
Hexamer
Frage 31
Frage
What is the role of Preofoldin in Archea?
Antworten
-
Captures nascent polypeptides and binds them to Gro-El
-
Captures nascent polypeptides and binds them to CCT
-
Captures nascent polypeptides and binds them to SSA
Frage 32
Frage
In Eukaryotes, prefoldin might be specific for the folding of what?
Antworten
-
Actin/tubulin
-
CCT
-
Hsp40
-
Hsp70
Frage 33
Frage
In archea there are 6 Prefoldin genes (Grim1-6) in Eukaryotes there are 2 (Gimalpha and Gimbeta)
Antworten
- True
- False
Frage 34
Frage
Which of the following describe Prefoldin
Antworten
-
Jellyfish like structure
-
With 6 coiled-coil beta sheets
-
With 6 coiled-coil alpha helices
Frage 35
Frage
There are 3.5 amino acid residues per helical turn of the coiled coil helices in Prefoldin (not 3.6)
Antworten
- True
- False
Frage 36
Frage
The subunits forming the dimerisation interface of the below molecule are what?
Image:
Prefolding (image/png)
Antworten
-
Hydrophobic
-
Hydrophillic
Frage 37
Frage
Where do the non-native proteins bind to Prefoldin?
Antworten
-
Exposed hydrophobic residues in the distal ends of the 'tentacle'
-
Exposed hydrophobic residues in the proximal ends of the 'tentacle'
-
Exposed hydrophobic residues at the top of the 'tentacle'
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