457002
Beschreibung
Quiz von gina_evans0312, aktualisiert more than 1 year ago
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Erstellt von gina_evans0312
vor fast 11 Jahre
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Frage 1
Frage
Name the two methods of physical fragmentation
Antworten
-
Shearing- filtering through a fine needed
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Sonication
-
Acidification
-
Boiling
Frage 2
Frage
Non-random shearing involves the use of helicases
Antworten
- True
- False
Frage 3
Frage
Which of the following cuts most frequently?
Antworten
-
4 cutters
-
6 cutters
-
8 cutters
Frage 4
Frage
A 4 cutter cuts a 4 bp sequence, and so on
Antworten
- True
- False
Frage 5
Frage
The staggered ends of two different enzymes can be ligated together, but afterwards cannot be cut by the same enzymes
Antworten
- True
- False
Frage 6
Frage
When dealing with DNA-cutting enzymes, what does the word degenerate mean?
Antworten
-
The ends they produce don't last long
-
They can cut more than one sequence
-
Where in the sequence they cut changes
Frage 7
Frage
What conditions are necessary for a partial digest?
Antworten
-
Not enough time
-
Less enzyme
-
Wrong enzyme conditions
-
A four cutter
Frage 8
Frage
Why could a partial digest be done?
Antworten
-
To get longer fragments and more likely preserve the gene
-
To save time
-
To prevent the DNA from degrading
Frage 9
Frage
Why is E.Coli most often used as a cloning vector?
Antworten
-
It has a rapid generation time
-
Its DNA is easy to manipulate
-
Its DNA is easy to get hold of
-
They don't mutate much
Frage 10
Frage
Bacteriophages can be used to transplant vectors into E.Coli
Antworten
- True
- False
Frage 11
Frage
How does M13 infect the male E.Coli?
Antworten
-
Binds to pilus surface
-
Enters through bacterial pores
-
Direct injection of DNA
Frage 12
Frage
Once inside the cell, the ssDONA of M13...
Antworten
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Is convered to dsDNA that acts like a plasmid
-
Is integrated into the bacterial chromosome
-
Is cut up by restriction enzymes ans integrated into the bacterial chromosome
Frage 13
Frage
TEVEN and Lambda enter the bacteria directly through the pore
Antworten
- True
- False
Frage 14
Frage
What is the point of the resistance genes in this Generation 1 plasmid
Image:
Gen_1_Plasmid (image/jpg)
Antworten
-
Tetracycline is interrupted- E.Coli with no resistance have had the recombinant plasmid inserted
-
The recombinant DNA is inserted at the EcrR1 site
-
E.Coli with ampicillin resistance have taken up a version of the plasmid
Frage 15
Antworten
-
The small subunit of LacZ
-
The large subunit of LacZ
-
Ampicillin
-
Tetracycline
Frage 16
Antworten
-
Monomer
-
Dimer
-
Tetramer
-
Hexamer
Frage 17
Frage
For Gen 2 plasmid testing to work, the LacZ gene must be removed from the E.Coli, and a plasmid for the large LacZ subunit added
Antworten
- True
- False
Frage 18
Frage
Why is the dish impregnated with X-Gal?
Antworten
-
It goes from blue to white in the presence of LacZ
-
It goes from white to blue in the presence of LacZ
-
It goes from white to blue in the presence of the small subunit of LacZ
Frage 19
Frage
If the gene is successfully inserted, the colony will remain white
Antworten
- True
- False
Frage 20
Frage
A Phagemid is virally inserted DNA in plasmid form
Antworten
- True
- False
Frage 21
Frage
Why would a vector be given an M13 origin and inserted with M13?
Antworten
-
The M13 phage will treat the plasmid as its own DNA and replicate it for secretion
-
The phage will insert the vector into the chromosome
-
The phage will prevent the bacteria from destroying the plasmid
Frage 22
Frage
Cosmids are plasmids with...?
Antworten
-
Cos lambda sites
-
Cos beta sites
-
Cos gamma sites
Frage 23
Frage
Which of the following has the largest limit?
Antworten
-
BAC's
-
YAC's
-
MAC's
Frage 24
Frage
Why do you remove a phosphate from the ends of bacterial DNA using a phosphatase?
Image:
Remove_P (image/png)
Antworten
-
To prevent it self ligating
-
To prevent it degrading
-
To prevent it getting destroyed
Frage 25
Frage
Why mix enzyme T4 and ATP together with the DNA to be inserted?
Antworten
-
Adding AMP to ligated ends gives easily ligable high energy form
-
ATP & T4 remove the phosphates from the ends of the DNA to prevent self annealing
-
T4 can only ligate DNA in the presence of ATP
Frage 26
Frage
A vector must be used because E.Coli cannot take up linear DNA
Antworten
- True
- False
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