457038
Beschreibung
Quiz von gina_evans0312, aktualisiert more than 1 year ago
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Erstellt von gina_evans0312
vor mehr als 10 Jahre
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Frage 1
Frage
Electroporations- passing electricity through a mix of DNA and bacteria causes DNA to be taken up
Antworten
- True
- False
Frage 2
Antworten
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The no of clones needed
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P of getting a clone with the desired gene
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Genome size
Frage 3
Antworten
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i = Library size g= probability of getting desired gene
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i = avg insert size g = genome size
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i = probability of getting desired gene g = genome size
Frage 4
Frage
The larger the genome, the larger the library required to replicate it
Antworten
- True
- False
Frage 5
Frage
When is cDNA used?
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When an organism has a lot of non-coding DNA
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When an organism is very small
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When the DNA is degraded
Frage 6
Frage
How is cDNA made?
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Converted from mRNA
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Converted from snoRNA
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Converted from siRNA
Frage 7
Frage
Less than 1% of the genome is mRNA, but it's exclusively expressible genes
Antworten
- True
- False
Frage 8
Antworten
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dNTP's
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Primer
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Reverse Transcriptase
Frage 9
Frage
What are the two methods of degrading the RNA part?
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Alkylisation (RNA is less stable)
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RNA digestion through RNAase H
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Boiling
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Restriction enzymes
Frage 10
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Alkali degrading
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Boiling
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Shaking
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RNAase H
Frage 11
Antworten
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dNTP's
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DNA Polymerase
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Primer
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Reverse transcriptase
Frage 12
Antworten
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RNAase H
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Alkylation
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Boiling
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Sonication
Frage 13
Antworten
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dNTP's
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DNA Polymerase H
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RNAase
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rNTP's
Frage 14
Frage
When creating cDNA, a little information is always lost from the 3' end
Antworten
- True
- False
Frage 15
Frage
How do we prevent this loss of information?
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After degrading the RNA, add a homopolymer (polyC) tail to the 3 end
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Using Terminal Transferase
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Before degrading the DNA, degrade the Poly A tail
Frage 16
Frage
Then in second strand synthesis add ...
Antworten
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dNTP's
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DNA polymerase
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Oligo-dG primer
Frage 17
Frage
Enriching desired DNA means creating/finding more of it
Antworten
- True
- False
Frage 18
Frage
Selective Hybridisation removes cDNA's common to more than one tissue
Antworten
- True
- False
Frage 19
Frage
Tissue Specificity is useful if the gene is only expressed in a certain tissue
Antworten
- True
- False
Frage 20
Frage
What is Normalisation?
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Melt, anneal and isolate ssDNA
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Use restriction enzymes to repeatdely cut and ligate DNA
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Use DNA helicase to continually replicate DNA
Frage 21
Frage
How does Normalisation work?
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Repetitive DNA binds to other repetitive DNA
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Leaving only unique DNA single stranded
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