Creating an E.Coli Library

Beschreibung

Year 2 Quiz am Creating an E.Coli Library, erstellt von gina_evans0312 am 29/12/2013.
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Quiz von gina_evans0312, aktualisiert more than 1 year ago
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Erstellt von gina_evans0312 vor fast 11 Jahre
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Zusammenfassung der Ressource

Frage 1

Frage
Electroporations- passing electricity through a mix of DNA and bacteria causes DNA to be taken up
Antworten
  • True
  • False

Frage 2

Frage
What does N stand for?
Antworten
  • The no of clones needed
  • P of getting a clone with the desired gene
  • Genome size

Frage 3

Frage
What are the meanings of i and g?
Antworten
  • i = Library size g= probability of getting desired gene
  • i = avg insert size g = genome size
  • i = probability of getting desired gene g = genome size

Frage 4

Frage
The larger the genome, the larger the library required to replicate it
Antworten
  • True
  • False

Frage 5

Frage
When is cDNA used?
Antworten
  • When an organism has a lot of non-coding DNA
  • When an organism is very small
  • When the DNA is degraded

Frage 6

Frage
How is cDNA made?
Antworten
  • Converted from mRNA
  • Converted from snoRNA
  • Converted from siRNA

Frage 7

Frage
Less than 1% of the genome is mRNA, but it's exclusively expressible genes
Antworten
  • True
  • False

Frage 8

Frage
What is required to make the first RNA-DNA hybrid?
Antworten
  • dNTP's
  • Primer
  • Reverse Transcriptase

Frage 9

Frage
What are the two methods of degrading the RNA part?
Antworten
  • Alkylisation (RNA is less stable)
  • RNA digestion through RNAase H
  • Boiling
  • Restriction enzymes

Frage 10

Frage
Which method was used to get the following DNA strand?
Antworten
  • Alkali degrading
  • Boiling
  • Shaking
  • RNAase H

Frage 11

Frage
What is required to get from the former to the latter?
Antworten
  • dNTP's
  • DNA Polymerase
  • Primer
  • Reverse transcriptase

Frage 12

Frage
What method was used to degrade the RNA into the following?
Antworten
  • RNAase H
  • Alkylation
  • Boiling
  • Sonication

Frage 13

Frage
What is needed to get from the former to the latter?
Antworten
  • dNTP's
  • DNA Polymerase H
  • RNAase
  • rNTP's

Frage 14

Frage
When creating cDNA, a little information is always lost from the 3' end
Antworten
  • True
  • False

Frage 15

Frage
How do we prevent this loss of information?
Antworten
  • After degrading the RNA, add a homopolymer (polyC) tail to the 3 end
  • Using Terminal Transferase
  • Before degrading the DNA, degrade the Poly A tail

Frage 16

Frage
Then in second strand synthesis add ...
Antworten
  • dNTP's
  • DNA polymerase
  • Oligo-dG primer

Frage 17

Frage
Enriching desired DNA means creating/finding more of it
Antworten
  • True
  • False

Frage 18

Frage
Selective Hybridisation removes cDNA's common to more than one tissue
Antworten
  • True
  • False

Frage 19

Frage
Tissue Specificity is useful if the gene is only expressed in a certain tissue
Antworten
  • True
  • False

Frage 20

Frage
What is Normalisation?
Antworten
  • Melt, anneal and isolate ssDNA
  • Use restriction enzymes to repeatdely cut and ligate DNA
  • Use DNA helicase to continually replicate DNA

Frage 21

Frage
How does Normalisation work?
Antworten
  • Repetitive DNA binds to other repetitive DNA
  • Leaving only unique DNA single stranded
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