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In Flow Cytometry, cell size is measured as they pass through laser beams
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Why is this area left?
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In Flow Cytometry, the Ig tested for varies depending on which cell you're measuring
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For which cell would you use an alkaline reagent?
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Eosinophils
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Basophils
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Monocytes
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Normal range of W.B.C's is 4-11 (x10^7/l)
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What is to be expected in a Settling test from a leukaemia patient?
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Why is a strong lysing solution added to a W.B.C count?
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Remove R.B.C's
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Remove platelets
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Like R.B.C's, W.B.C's are counted using current impedence
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What are the two populations (peaks) shown here?
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Lymphocytes
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Monocytes
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Nutraphils
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All others
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What two things are mapped in D.I.F.F?
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Size
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Shape
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Granularity
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Markers
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What cells are being pointed at on the graph?
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Netraphils
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Monocytes
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Lymphocytes
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Cellpack & Stromatolyers 3D do what?
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Protect W.B.C's
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Speed R.B.C's lysis
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List some causes of Neutropenia
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Chronic infection/inflammation and increased cytokinase can lead to high...
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Monocytes
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Eosinophils
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Basophils
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Basophilia is caused by
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Leukaemias
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Allergic recations
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Eosinophilia is caused by allergic infections, parasitic infections and drug hypersensitivity
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Name some causes of Lymhpocytopenia
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Name the three tests done for any Leucocytic disorder
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Flow Cytometry
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Full blood count & DFF
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NAP test
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Paul Bunnell Test
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Morphology
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Why does Clonality indicate a severe pathology?
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What markers do you test for when looking for Clonality?
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Kappa/lambda markers
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CD4/CD8
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Alpha, beta & gamma
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In AIDS, you perform flow cytometry for Kappa/Lambda markers
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The Paul Bunnel test is done for...
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Name the cause of Haematological Malignancies
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Genetics
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Drugs
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Irradiation