Enterobacteriaceae

Beschreibung

All about enterobacteriaceae
Katie Koontz
Quiz von Katie Koontz, aktualisiert more than 1 year ago
Katie Koontz
Erstellt von Katie Koontz vor etwa 8 Jahre
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Zusammenfassung der Ressource

Frage 1

Frage
Purpose: TSI agar can be considered an initial step in the identification of the [blank_start]Enterobacteriaceae[blank_end]
Antworten
  • Enterobacteriaceae
  • Salmonella typhi
  • Escherichia coli
  • Citrobacter diversus

Frage 2

Frage
Principle: for [blank_start]Indole Broth[blank_end]: tryptophan PRESENT in peptone is oxidized y certain bacteria to indole, skatole, and indoleacetic acid. Indole is detected in broth cultures of bacteria with an alcoholic p-dimethylaminobenzldehyde reagent. Indole reacts with the aldehyde to form a [blank_start]red[blank_end] product.
Antworten
  • Indole Broth
  • Urea Agar
  • Voges-proskauer
  • Methyl red
  • red
  • black
  • yellow
  • gray

Frage 3

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Key Ingredients: [blank_start]Indole broth[blank_end]: Tryptophan (1% broth), Kovac's reagent or Ehrlich's reagent, Xylene or chloroform for extraction if using Ehrlich's reagent
Antworten
  • Indole broth
  • Methyl Red
  • Phenylalanine
  • LIA

Frage 4

Frage
Indole Broth Reactions: Negative Reaction: [blank_start]No[blank_end] color development Positive Reaction: [blank_start]red[blank_end] ring at the interfacec of reagent and broth) Variable reaction: [blank_start]Orange[blank_end] color, indicates production of aktole, a methylated intermediate that may be a precursor to indole production
Antworten
  • No
  • Red
  • Dark
  • Yellow
  • red
  • yellow
  • no
  • brown
  • Orange
  • Red
  • Black
  • Purple

Frage 5

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Principle: Urea Hydrolysis (Christensen's Agar): Many organisms possess the enzyme urease which can hydrolyze urea with the release of ammonia and carbon dioxide which acts to increase the pH of the medium and turn the indicator phenol red from pale [blank_start]orange[blank_end] to dark [blank_start]fuchsia pink[blank_end]
Antworten
  • orange
  • fuchsia pink
  • violet purple
  • green
  • fuchsia pink
  • orange
  • violet purple
  • green

Frage 6

Frage
Key Ingredients: Urea Agar Urea Agar ([blank_start]christensen's Base[blank_end]) pH indicator = [blank_start]phenol red[blank_end]
Antworten
  • christensen's Base
  • methyl red
  • spot indole broth
  • citrate
  • phenol red
  • methyl red
  • gelatin
  • API 20E strip

Frage 7

Frage
Interpretation: urea hydrolysis A strong positive hydrolysis test: entire agar, slant and butt, turning [blank_start]deep pink[blank_end] after overnight incubation. A moderate positive test: [blank_start]pink[blank_end] slant at 24 hours, with the intensity of the color increasing with extended incubation A negative test: no change from pale [blank_start]orange[blank_end] color of the slant
Antworten
  • deep pink
  • deep purple
  • light orange
  • light pink
  • pink
  • orange
  • yellow
  • red
  • orange
  • pink
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