Zusammenfassung der Ressource
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Purpose: TSI agar can be considered an initial step in the identification of the [blank_start]Enterobacteriaceae[blank_end]
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Enterobacteriaceae
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Salmonella typhi
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Escherichia coli
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Citrobacter diversus
Frage 2
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Principle: for [blank_start]Indole Broth[blank_end]: tryptophan PRESENT in peptone is oxidized y certain bacteria to indole, skatole, and indoleacetic acid. Indole is detected in broth cultures of bacteria with an alcoholic p-dimethylaminobenzldehyde reagent. Indole reacts with the aldehyde to form a [blank_start]red[blank_end] product.
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Indole Broth
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Urea Agar
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Voges-proskauer
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Methyl red
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red
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black
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yellow
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gray
Frage 3
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Key Ingredients: [blank_start]Indole broth[blank_end]: Tryptophan (1% broth), Kovac's reagent or Ehrlich's reagent, Xylene or chloroform for extraction if using Ehrlich's reagent
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Indole broth
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Methyl Red
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Phenylalanine
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LIA
Frage 4
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Indole Broth Reactions:
Negative Reaction: [blank_start]No[blank_end] color development
Positive Reaction: [blank_start]red[blank_end] ring at the interfacec of reagent and broth)
Variable reaction: [blank_start]Orange[blank_end] color, indicates production of aktole, a methylated intermediate that may be a precursor to indole production
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No
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Red
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Dark
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Yellow
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red
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yellow
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no
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brown
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Orange
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Red
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Black
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Purple
Frage 5
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Principle: Urea Hydrolysis (Christensen's Agar): Many organisms possess the enzyme urease which can hydrolyze urea with the release of ammonia and carbon dioxide which acts to increase the pH of the medium and turn the indicator phenol red from pale [blank_start]orange[blank_end] to dark [blank_start]fuchsia pink[blank_end]
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orange
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fuchsia pink
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violet purple
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green
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fuchsia pink
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orange
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violet purple
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green
Frage 6
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Key Ingredients: Urea Agar
Urea Agar ([blank_start]christensen's Base[blank_end])
pH indicator = [blank_start]phenol red[blank_end]
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christensen's Base
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methyl red
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spot indole broth
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citrate
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phenol red
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methyl red
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gelatin
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API 20E strip
Frage 7
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Interpretation: urea hydrolysis
A strong positive hydrolysis test: entire agar, slant and butt, turning [blank_start]deep pink[blank_end] after overnight incubation.
A moderate positive test: [blank_start]pink[blank_end] slant at 24 hours, with the intensity of the color increasing with extended incubation
A negative test: no change from pale [blank_start]orange[blank_end] color of the slant
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deep pink
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deep purple
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light orange
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light pink
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pink
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orange
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yellow
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red
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orange
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pink