Question 1
Question
Obligate intracellular parasite
Answer
-
Organisms that can only grow inside another living host.
-
Viruses that infect bacteria.
-
Viral replication cycle that ends in the death of the host cell.
Question 2
Answer
-
Viruses that infect bacteria.
-
Organisms that can only grow inside another living host cell.
-
Viral replication cycle that ends in death of its host cell.
Question 3
Answer
-
Viral replication cycle that ends in death of its host cell.
-
Organisms that can only grow inside another living host cell.
-
Viruses that infect bacteria.
Question 4
Question
How does a medium used for bacterial growth differ from media used for bacteriophage growth?
Answer
-
Use ordinary media to grow bacteria but, a bacteriophage needs a host cell.
-
Use ordinary media to grow bacteriophages but, bacteria needs a host cell.
Question 5
Question
What is the purpose of making serial dilutions of the T-4 phage?
Answer
-
To obtain a countable plate of 30 - 300 plaques.
-
To obtain a countable plate of 30 - 300 colonies.
-
To obtain a countable plate of 30 - 300 individual viruses.
Question 6
Question
What is a viral plaque and what does it represent?
Answer
-
An area of clearing on an agar representing a spot where a virus landed, infected a bacteria and lysed it.
-
An area on an agar representing a spot where a bacteria landed, infected a virus and lysed it.
-
An area on an agar representing a spot where a virus landed, infect a yeast and lysed it.
Question 7
Question
What purpose does the E. coli serve in this procedure?
Answer
-
It is the host cell for the T4 bacteriophage
-
It is the host cell of the T4 bacteria.
-
It is the host cell of the T4 fungus.
Question 8
Question
Explain why Coccidioides immitis is considered to be a dimorphic fungus.
Answer
-
It can grow as a yeast and as a mold.
-
It can grow as a yeast and a virus.
-
It can grow as a mold and a virus.
Question 9
Question
What are the growth differences between fungus and bacteria?
Answer
-
Fungi grow faster, at a lower temperature and at a lower pH than bacteria.
-
Fungi grow faster, at a higher temperature and at a higher pH than bacteria.
-
Fungi grow slower, at a lower temperature and at a lower pH than bacteria.
-
Fungi grow slower, at a higher temperature and at a higher pH than bacteria.
Question 10
Question
Staphylococcus and Micrococcus are"
Answer
-
Gram (+) cocci in clusters
-
Gram (-) cocci in clusters
-
Staphylococcus is gram (+) in clusters and Micrococcus is gram (-) in pairs
-
Staphylococcus is gram (-) in clusters and Micrococcus is gram (+) in pairs
Question 11
Question
Where are Staphylococcus and Micrococcus normally found in the body?
Answer
-
Skin and mucus membranes
-
Intestines
-
Gastrointestinal tract
-
Skin and hair
Question 12
Question
Where are Staphylococcus and Micrococcus normally found in the body?
Answer
-
Skin and mucus membranes
-
Intestines
-
Gastrointestinal tract
-
Skin and hair
Question 13
Question
People that harbor the pathogen but exhibit no symptoms.
Answer
-
Carrier
-
MRSA
-
Nosocomial
-
Giver
Question 14
Question
Methicillin-resistant Staphylococcus aureus
Question 15
Question
Hospital acquired
Answer
-
Nosocomial
-
Carrier
-
Giver
-
Catalase
Question 16
Question
Which substances that Staphylococcus aureus produce to cause disease
Answer
-
Catalase
-
Coagulase
-
Hyaluronidase
-
Carilase
Question 17
Question
When do staphylococcus epidermidis and Micrococcus cause disease?
Question 18
Question
Differentiate the colony appearance of Staphylococcus and Streptococcus
Answer
-
Staph are larger, convex, opague and pigmented, Strep are smaller, translucent to slightly opaque.
-
Strep are larger, convex, opague and pigmented, Straph are smaller, translucent to slightly opaque.
-
Staph are smaller, convex, opague and pigmented, Strep are larger, translucent to slightly opaque.
Question 19
Question
Which enzyme helps differentiate all staphylococci from streptococci?
Answer
-
Catalase
-
Streptocase
-
Staphoolase
Question 20
Question
How did Lancefield divide the beta-hemolytic streptococci into groups?
Answer
-
C polysaccharide extracted from the cell walls
-
Peptidoglycan extracted from the cell walls
-
Lipids extracted from the cell walls
Question 21
Question
What is the species name of Lancefield Group A streptococci.
Question 22
Question
What is the species name of Lancefield Group B streptococci?
Answer
-
Streptococcus agalaticae
-
Streptococcus aureus
-
Streptococcus pyogenes
-
Streptococcus cocci
Question 23
Question
Where are Enterococcus normally found int he body?
Answer
-
Gastrointestinal tract
-
Skin and mucus
-
Liver
-
Kidneys
Question 24
Answer
-
Vancomycin resistant enterococcus
-
Vancomycin resistant ebolacoccus
-
Vancomycin resistant enteroboccus
Question 25
Question
Viridans streptococci
Answer
-
Alpha hemolytic streptococci other than streptococcus pneumonea
-
Beta hemolytic streptococci other than streptococcus pneumonea
-
Alpha heolytic staphylococci other than staphylococcus pneumonea
-
Beta heolytic staphylococci other than staphylococcus pneumonea
Question 26
Question
Group A Strep causes __________________
Question 27
Question
Group B Strep causes _____________
Question 28
Question
Enterococcus causes _________________
Answer
-
Urinary tract infection
-
Dental cavities
-
Strep throat
-
Neonatal speticemia
Question 29
Question
Streptococcus pneumoniae causes _________________
Question 30
Question
Viridans streptococci
Answer
-
Dental cavities
-
Strep throat
-
Urinary tract infection
-
Neonatal septicemia
Question 31
Question
Why is Coccidioides immitis considered to be a dimorphic fungus?
Question 32
Question
What are the growth factors between fungus and bacteria?
Answer
-
Fungi grow slower, at a lower temp and lower pH than bacteria
-
Fungi grow faster, at a higher temp and higher pH than bacteria
-
Fungi grow slower, at a higher temp and lower pH than bacteria
-
Fungi grow faster, at a lower temp and higher pH than bacteria
Question 33
Question
Explain the difference between vegetative hyphae and aerial hyphae.
Question 34
Question
The difference between sporangiospores and conidiospores:
Question 35
Question
How are molds identified in the laboratory?
Answer
-
Macroscopic appearance
-
Microscopic appearance
-
Hyphal structure
Question 36
Question
What are the characteristics of Enterobacteriacea?
Question 37
Question
What are the characteristics of non-fermenters?
Question 38
Question
What are the two major groups of gram (-) rods?
Answer
-
Enterobacteriacaea
-
Non-fermenters
-
Staphylococcus
-
Streptococcus
Question 39
Question
Which carbohydrate is used to determine whether a gram negative rod is a "Nonfermenter" or "Enterobacteriacae"?
Question 40
Question
What carbohydrate(s) are in the Kigler Iron Agar media?
Answer
-
Glucose
-
Lactose
-
Sucrose
-
Galactose
Question 41
Question
What carbohydrate(s) are in the MacConkey agar?
Answer
-
Glucose
-
Lactose
-
Sucrose
-
Galactose
Question 42
Question
Why is MacConkey agar considered to be a selective and differential media?
Answer
-
It is selective because it contains bile salts and crystal violet which inhibit the growth of gram (+) organisms. It is differential because it contains lactose making it differential for lactose fermentation.
-
It is selective because it contains bile salts and crystal violet which inhibit the growth of gram (-) organisms. It is differential because it contains lactose making it differential for lactose fermentation.
-
It is differential because it contains bile salts and crystal violet which inhibit the growth of gram (+) organisms. It is selective because it contains lactose making it differential for lactose fermentation.
-
It is differential because it contains bile salts and crystal violet which inhibit the growth of gram (-) organisms. It is selective because it contains lactose making it differential for lactose fermentation.
Question 43
Question
How many types of organisms will you identify in the mixed unknown exercise?
Question 44
Question
What type of organism grows on MacConkey agar?
Question 45
Question
What ingredient on the MacConkey agar will differentiate the organisms growing on the agar?
Question 46
Question
What type of organism grows on CNA?
Question 47
Question
What ingredient in the CNA media allows differentiation of the organisms growing on the agar?
Question 48
Question
Why is CNA media incubated in a candle jar?
Question 49
Question
Why is a cotton swab used to obtain the sample from the original broth?
Answer
-
To make sure we get both organisms from the mixed culture
-
To make sure enough sample is collected
-
To make it easier to inoculate the media
Question 50
Question
Why is T. soy agar used to grow the "stock cultures"?
Answer
-
It's a neutral media that all bacteria like
-
It is a selective media that only gram (-) bacteria like.
-
It is a selective media that only gram (+) bacteria like.
Question 51
Question
Why do you need to develop two flow charts in order to identify your unknown organisms?
Answer
-
You have to develop a dichotomous key for each of your unknown organisms and we have two organisms.
-
You have to develop a dichotomous key for each depending on the outcome of growth on the selective media.
Question 52
Question
What is an "enteric" organism?
Question 53
Question
How are culture media designed to detect if a bacteria can produce a specific enzyme?
Answer
-
Substrates are incorporated into the culture media that the enzymes act upon and that along with indicator system lets you see the products.
-
Enzymes are incorporated into the media and an indicator systems lets you see if they match the organism.
Question 54
Question
What is the purpose of the Durham tube in the carbohydrate fermentation tubes?
Question 55
Question
Name the specific tests that are part of the IMViC test
Answer
-
Indole
-
Methyl red
-
Voges-Proskauer
-
Citrate
-
Urease
Question 56
Question
Name 3 Enterobacteriaceae normally found in the gastrointestinal tract.
Answer
-
E. coli
-
Enterobacter aerogenes
-
Proteus vulgaris
-
Salmonella
-
Klebsiella pneumoniae
-
Shigella
Question 57
Question
Name two Enterobacteriaceae that are major pathogens and state the disease they cause.
Answer
-
Salmonella - Gastroenteritis, typhoid fever
-
Shigella - Bacterial dysentery
-
E. coli - Gastroenteritis, typhoid fever
-
Proteus vulgaris - Bacterial dysentery
Question 58
Question
Which commercial test systems are used to identify gram (-) rods in clinical labs?
Answer
-
Entero tube
-
API
-
20E
-
Micro ID
-
Micro tube
-
APE
Question 59
Question
Why do you use the streak-stab method for blood agar inoculations?
Question 60
Question
Where do you grow streptococcus and why?
Question 61
Question
Which bacteria are alpha and optochin sensitive?
Answer
-
Streptococcus pneumoniae
-
Streptococcus mitis
-
Streptococcus pyogenes
-
Group C Streptococcus
-
Enterococcis faecalis
Question 62
Question
Which bacteria are and optochin resistant?
Answer
-
Streptococcus mitis
-
Streptococcus pneumoniae
-
Streptococcus pyogenes
-
Group C Streptococcus
-
Enterococcus faecalis
Question 63
Question
Which bacteria are beta and bacitracin sensitive?
Answer
-
Streptococcus pyogenes
-
Streptococcus pneumoniae
-
Streptococcus mitis
-
Group C Streptococcus
-
Enterococcus faecalis
Question 64
Question
Which bacteria are beta and bacitracin resistant?
Answer
-
Group C Streptococcus
-
Streptococcus mitis
-
Streptococcus pneumoniae
-
Enterococcus faecalis
-
Streptococcus pyogenes
Question 65
Question
How do you tell the difference between the alpha hemolytic Streptococcus and and Enterococcus faecalis?
Question 66
Question
How does the catalase test differentiate between Staphylococcus and Streptococcus?
Question 67
Question
What kind of hemolysis is shown on this plate?
Answer
-
Alpha hemolyisis
-
Beta Hemolysis
-
Gamma Hemolysis
Question 68
Question
What is the mold pictured?
Answer
-
Aspergillis
-
Penicillium
-
Rhizopus
Question 69
Question
What is the mold pictured?
Answer
-
Aspergillus
-
Penicillium
-
Rhizopus
Question 70
Question
What is the mold pictured?
Answer
-
Aspergillus
-
Penicillium
-
Rhizopus
Question 71
Question
What is the mold pictured?
Answer
-
Aspergillus
-
Penicillium
-
Rhuzopus
Question 72
Question
What is the mold pictured?
Answer
-
Aspergillus
-
Penicillium
-
Rhizopus
Question 73
Question
What is the mold pictured?
Answer
-
Aspergillus
-
Penicillium
-
Rhizopus
Question 74
Question
What is the mold pictured?
Answer
-
Aspergillus
-
Penicillium
-
Rhizopus
Question 75
Question
What mold is pictured?
Answer
-
Aspergillus
-
Penicillium
-
Rhizopus
Question 76
Question
What mold is pictured?
Answer
-
Aspergillus
-
Penicillium
-
Rhizopus
Question 77
Question
What mold is pictured?
Answer
-
Aspergillus
-
Penicillium
-
Rhizopus
Question 78
Question
What mold is pictured?
Answer
-
Aspergillus
-
Penicillium
-
Rhizopus
Question 79
Question
What mold is pictured?
Answer
-
Aspergillus
-
Penicillium
-
Rhizopus
Question 80
Question
What mold is pictured?
Answer
-
Aspergillus
-
Penicillium
-
Rhizopus
Question 81
Question
What mold is pictured?
Answer
-
Aspergillus
-
Penicillium
-
Rhizopus
Question 82
Question
What mold is pictured?
Answer
-
Aspergillus
-
Penicillium
-
Rhizopus
Question 83
Question
Is this result positive or negative for beta lactamase?
Question 84
Question
Is this result positive or negative for beta lactamase?
Question 85
Question
What are the results of the bile esculin test pictured?
Answer
-
A = negative, B = positive
-
A = positive, B = negative
Question 86
Question
What are the results of the carbohydrate fermentation pictured?
Answer
-
A = negative, B = positive, C = positive + gas
-
A = positive, B = negative, C = negative +gas
Question 87
Question
What are the results of the citrate test pictured?
Answer
-
A = negative, B = positive
-
A = positive, B = negative
Question 88
Question
What are the results of the indole test pictured?
Answer
-
A = negative, B = positive
-
A = positive, B = negative
Question 89
Question
What is the result of the lactose fermentation pictured?
Answer
-
A = negative, B = positive, C = positive
-
A = negative, B = negative, C = positive
-
A = positive, B = negative, C = negative
-
A = positive, B = positive, C = negative
Question 90
Question
What are the results of the motility test pictured?
Answer
-
A = non-motile, B = motile, C = motile
-
A = non-motile, B = non-motile, C = motile
-
A = motile, B =motile, C = non-motile
-
A = motile, B = non-motile, C = non-motile
Question 91
Question
What are the results of the sensitivity test pictured?
Answer
-
A = sensitive, B = resistant
-
A = resistant, B = sensitive
Question 92
Question
How do you calculate the titer of phage in the original sample given the number of plaques and the dilution of the plate?
Question 93
Question
What kind of hemolysis is pictured?
Question 94
Question
Which bacteria is cogulase positive and manitol slat agar positive?
Question 95
Question
Which bacteria is coagulase negative and manitol salt agar negative?
Question 96
Question
What bacteria is positive for nitrate reduction and rose for pigment production?
Question 97
Question
What bacteria is negative for nitrate reduction and yellow for pigment production?
Question 98
Question
How is Enterococcus faecalis differentiated from other alpha hemolytic streptococcus?
Question 99
Question
Which Streptococcus bacteria is optochin resistant?
Answer
-
Streptococcus mitis
-
Streptococcus pneumoniae
-
Streptococcus pyogenes
-
Group C streptococcus
Question 100
Question
Which streptococcus bacteria is optochin sensitive?
Answer
-
Streptococcus pneumoniae
-
Streptococcus mitis
-
Streptococcus pyogenes
-
Group C Streptococcus
Question 101
Question
Which bacteria is bacitration resistant?
Answer
-
Group C Streptococcus
-
Streptococcus pyogenes
-
Streptococcus pneumoniae
-
Streptococcus mitis
Question 102
Question
Which bacteria is bacitracin sensitive?
Answer
-
Streptococcus pyogenes
-
Streptococcus mitis
-
Streptococcus pneumoniae
-
Group C Streptococcus
Question 103
Question
Which bacteria are alpha hemolytic?
Answer
-
Streptococcus mitis
-
Streptococcus pneumoniae
-
Streptococcus pyogenes
-
Group C Streptococcus
Question 104
Question
Which bacteria are beta hemolytic?
Answer
-
Streptococcus mitis
-
Streptococcus pneumoniae
-
Streptococcus pyogenes
-
Group C Streptococcus
Question 105
Question
What hemolysis is pictured?
Answer
-
A= alpha, B = beta, C = gamma
-
A = beta, B = alpha, C = gamma
-
A = gamma, B = alpha, C = beta