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Microbiology Practical F

Beschreibung

Raymond MCC
Elle Ashe
Quiz von Elle Ashe, aktualisiert more than 1 year ago
Elle Ashe
Erstellt von Elle Ashe vor mehr als 10 Jahre
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Zusammenfassung der Ressource

Frage 1

Frage
Obligate intracellular parasite
Antworten
  • Organisms that can only grow inside another living host.
  • Viruses that infect bacteria.
  • Viral replication cycle that ends in the death of the host cell.

Frage 2

Frage
Bacteriophage
Antworten
  • Viruses that infect bacteria.
  • Organisms that can only grow inside another living host cell.
  • Viral replication cycle that ends in death of its host cell.

Frage 3

Frage
Lytic cycle
Antworten
  • Viral replication cycle that ends in death of its host cell.
  • Organisms that can only grow inside another living host cell.
  • Viruses that infect bacteria.

Frage 4

Frage
How does a medium used for bacterial growth differ from media used for bacteriophage growth?
Antworten
  • Use ordinary media to grow bacteria but, a bacteriophage needs a host cell.
  • Use ordinary media to grow bacteriophages but, bacteria needs a host cell.

Frage 5

Frage
What is the purpose of making serial dilutions of the T-4 phage?
Antworten
  • To obtain a countable plate of 30 - 300 plaques.
  • To obtain a countable plate of 30 - 300 colonies.
  • To obtain a countable plate of 30 - 300 individual viruses.

Frage 6

Frage
What is a viral plaque and what does it represent?
Antworten
  • An area of clearing on an agar representing a spot where a virus landed, infected a bacteria and lysed it.
  • An area on an agar representing a spot where a bacteria landed, infected a virus and lysed it.
  • An area on an agar representing a spot where a virus landed, infect a yeast and lysed it.

Frage 7

Frage
What purpose does the E. coli serve in this procedure?
Antworten
  • It is the host cell for the T4 bacteriophage
  • It is the host cell of the T4 bacteria.
  • It is the host cell of the T4 fungus.

Frage 8

Frage
Explain why Coccidioides immitis is considered to be a dimorphic fungus.
Antworten
  • It can grow as a yeast and as a mold.
  • It can grow as a yeast and a virus.
  • It can grow as a mold and a virus.

Frage 9

Frage
What are the growth differences between fungus and bacteria?
Antworten
  • Fungi grow faster, at a lower temperature and at a lower pH than bacteria.
  • Fungi grow faster, at a higher temperature and at a higher pH than bacteria.
  • Fungi grow slower, at a lower temperature and at a lower pH than bacteria.
  • Fungi grow slower, at a higher temperature and at a higher pH than bacteria.

Frage 10

Frage
Staphylococcus and Micrococcus are"
Antworten
  • Gram (+) cocci in clusters
  • Gram (-) cocci in clusters
  • Staphylococcus is gram (+) in clusters and Micrococcus is gram (-) in pairs
  • Staphylococcus is gram (-) in clusters and Micrococcus is gram (+) in pairs

Frage 11

Frage
Where are Staphylococcus and Micrococcus normally found in the body?
Antworten
  • Skin and mucus membranes
  • Intestines
  • Gastrointestinal tract
  • Skin and hair

Frage 12

Frage
Where are Staphylococcus and Micrococcus normally found in the body?
Antworten
  • Skin and mucus membranes
  • Intestines
  • Gastrointestinal tract
  • Skin and hair

Frage 13

Frage
People that harbor the pathogen but exhibit no symptoms.
Antworten
  • Carrier
  • MRSA
  • Nosocomial
  • Giver

Frage 14

Frage
Methicillin-resistant Staphylococcus aureus
Antworten
  • MRSA
  • Carrier
  • Nosocomial
  • Multiple Resistant Streptococcus Aureus

Frage 15

Frage
Hospital acquired
Antworten
  • Nosocomial
  • Carrier
  • Giver
  • Catalase

Frage 16

Frage
Which substances that Staphylococcus aureus produce to cause disease
Antworten
  • Catalase
  • Coagulase
  • Hyaluronidase
  • Carilase

Frage 17

Frage
When do staphylococcus epidermidis and Micrococcus cause disease?
Antworten
  • They are opportunistic pathogens.
  • All the time
  • Never

Frage 18

Frage
Differentiate the colony appearance of Staphylococcus and Streptococcus
Antworten
  • Staph are larger, convex, opague and pigmented, Strep are smaller, translucent to slightly opaque.
  • Strep are larger, convex, opague and pigmented, Straph are smaller, translucent to slightly opaque.
  • Staph are smaller, convex, opague and pigmented, Strep are larger, translucent to slightly opaque.

Frage 19

Frage
Which enzyme helps differentiate all staphylococci from streptococci?
Antworten
  • Catalase
  • Streptocase
  • Staphoolase

Frage 20

Frage
How did Lancefield divide the beta-hemolytic streptococci into groups?
Antworten
  • C polysaccharide extracted from the cell walls
  • Peptidoglycan extracted from the cell walls
  • Lipids extracted from the cell walls

Frage 21

Frage
What is the species name of Lancefield Group A streptococci.
Antworten
  • Streptococcus pyogenes
  • Streptococcus aureus
  • Streptococcus epidermidis
  • Streptococcus agalaticae

Frage 22

Frage
What is the species name of Lancefield Group B streptococci?
Antworten
  • Streptococcus agalaticae
  • Streptococcus aureus
  • Streptococcus pyogenes
  • Streptococcus cocci

Frage 23

Frage
Where are Enterococcus normally found int he body?
Antworten
  • Gastrointestinal tract
  • Skin and mucus
  • Liver
  • Kidneys

Frage 24

Frage
VRE?
Antworten
  • Vancomycin resistant enterococcus
  • Vancomycin resistant ebolacoccus
  • Vancomycin resistant enteroboccus

Frage 25

Frage
Viridans streptococci
Antworten
  • Alpha hemolytic streptococci other than streptococcus pneumonea
  • Beta hemolytic streptococci other than streptococcus pneumonea
  • Alpha heolytic staphylococci other than staphylococcus pneumonea
  • Beta heolytic staphylococci other than staphylococcus pneumonea

Frage 26

Frage
Group A Strep causes __________________
Antworten
  • Strep throat
  • Community-acquired pneumonia
  • Neonatal septicemia
  • Urinary tract infection

Frage 27

Frage
Group B Strep causes _____________
Antworten
  • Neonatal septicemia
  • Strep throat
  • Urinary tract infection
  • Community-acquired pneumonia

Frage 28

Frage
Enterococcus causes _________________
Antworten
  • Urinary tract infection
  • Dental cavities
  • Strep throat
  • Neonatal speticemia

Frage 29

Frage
Streptococcus pneumoniae causes _________________
Antworten
  • Community-acquired pneumonia
  • Neonatal septicemia
  • Urinary tract infection
  • Strep throat

Frage 30

Frage
Viridans streptococci
Antworten
  • Dental cavities
  • Strep throat
  • Urinary tract infection
  • Neonatal septicemia

Frage 31

Frage
Why is Coccidioides immitis considered to be a dimorphic fungus?
Antworten
  • It can grow as a yeast and as a mold
  • It can grow in pairs.
  • It can grow as coccus or bacillus.

Frage 32

Frage
What are the growth factors between fungus and bacteria?
Antworten
  • Fungi grow slower, at a lower temp and lower pH than bacteria
  • Fungi grow faster, at a higher temp and higher pH than bacteria
  • Fungi grow slower, at a higher temp and lower pH than bacteria
  • Fungi grow faster, at a lower temp and higher pH than bacteria

Frage 33

Frage
Explain the difference between vegetative hyphae and aerial hyphae.
Antworten
  • Vegetative hyphae grow on or down into the agar surface; aerial hyphae grow above the agar surface.
  • Aerial hyphae grow on or down into the agar surface; vegetative hyphae grow above the agar surface.

Frage 34

Frage
The difference between sporangiospores and conidiospores:
Antworten
  • Sporangipospores are inside of a sac and condidiospores are the end of hyphae.
  • Condidiospores are inside of a sac and sporangipospores are the end of hyphae.

Frage 35

Frage
How are molds identified in the laboratory?
Antworten
  • Macroscopic appearance
  • Microscopic appearance
  • Hyphal structure

Frage 36

Frage
What are the characteristics of Enterobacteriacea?
Antworten
  • All are gram (-) rods
  • All are gram (+) cocci
  • All ferment glucose
  • All reduce nitrate to nitrite
  • They are oxidase (-)
  • They are oxidase (+)
  • They do not ferment glucose

Frage 37

Frage
What are the characteristics of non-fermenters?
Antworten
  • All are gram (-)
  • All ferment glucose
  • They do not ferment glucose
  • They are all oxidase (-)
  • Many are oxidase (+)
  • All are gram (+)

Frage 38

Frage
What are the two major groups of gram (-) rods?
Antworten
  • Enterobacteriacaea
  • Non-fermenters
  • Staphylococcus
  • Streptococcus

Frage 39

Frage
Which carbohydrate is used to determine whether a gram negative rod is a "Nonfermenter" or "Enterobacteriacae"?
Antworten
  • Glucose
  • Lactose

Frage 40

Frage
What carbohydrate(s) are in the Kigler Iron Agar media?
Antworten
  • Glucose
  • Lactose
  • Sucrose
  • Galactose

Frage 41

Frage
What carbohydrate(s) are in the MacConkey agar?
Antworten
  • Glucose
  • Lactose
  • Sucrose
  • Galactose

Frage 42

Frage
Why is MacConkey agar considered to be a selective and differential media?
Antworten
  • It is selective because it contains bile salts and crystal violet which inhibit the growth of gram (+) organisms. It is differential because it contains lactose making it differential for lactose fermentation.
  • It is selective because it contains bile salts and crystal violet which inhibit the growth of gram (-) organisms. It is differential because it contains lactose making it differential for lactose fermentation.
  • It is differential because it contains bile salts and crystal violet which inhibit the growth of gram (+) organisms. It is selective because it contains lactose making it differential for lactose fermentation.
  • It is differential because it contains bile salts and crystal violet which inhibit the growth of gram (-) organisms. It is selective because it contains lactose making it differential for lactose fermentation.

Frage 43

Frage
How many types of organisms will you identify in the mixed unknown exercise?
Antworten
  • 1
  • 2
  • 4

Frage 44

Frage
What type of organism grows on MacConkey agar?
Antworten
  • Gram (-)
  • Gram (+)

Frage 45

Frage
What ingredient on the MacConkey agar will differentiate the organisms growing on the agar?
Antworten
  • Lactose
  • Bile salts and crystal violet
  • Blood

Frage 46

Frage
What type of organism grows on CNA?
Antworten
  • Gram (+)
  • Gram (-)

Frage 47

Frage
What ingredient in the CNA media allows differentiation of the organisms growing on the agar?
Antworten
  • Bile salts and crystal violet
  • Blood
  • Lactose

Frage 48

Frage
Why is CNA media incubated in a candle jar?
Antworten
  • Streptococcus are microaerophiles
  • Streptococcus are anaerobes

Frage 49

Frage
Why is a cotton swab used to obtain the sample from the original broth?
Antworten
  • To make sure we get both organisms from the mixed culture
  • To make sure enough sample is collected
  • To make it easier to inoculate the media

Frage 50

Frage
Why is T. soy agar used to grow the "stock cultures"?
Antworten
  • It's a neutral media that all bacteria like
  • It is a selective media that only gram (-) bacteria like.
  • It is a selective media that only gram (+) bacteria like.

Frage 51

Frage
Why do you need to develop two flow charts in order to identify your unknown organisms?
Antworten
  • You have to develop a dichotomous key for each of your unknown organisms and we have two organisms.
  • You have to develop a dichotomous key for each depending on the outcome of growth on the selective media.

Frage 52

Frage
What is an "enteric" organism?
Antworten
  • Part of the normal flora of the intestinal tract
  • Bacteria that live inside another organism.

Frage 53

Frage
How are culture media designed to detect if a bacteria can produce a specific enzyme?
Antworten
  • Substrates are incorporated into the culture media that the enzymes act upon and that along with indicator system lets you see the products.
  • Enzymes are incorporated into the media and an indicator systems lets you see if they match the organism.

Frage 54

Frage
What is the purpose of the Durham tube in the carbohydrate fermentation tubes?
Antworten
  • Collects gas
  • Releases gas
  • Keeps tube from exploding

Frage 55

Frage
Name the specific tests that are part of the IMViC test
Antworten
  • Indole
  • Methyl red
  • Voges-Proskauer
  • Citrate
  • Urease

Frage 56

Frage
Name 3 Enterobacteriaceae normally found in the gastrointestinal tract.
Antworten
  • E. coli
  • Enterobacter aerogenes
  • Proteus vulgaris
  • Salmonella
  • Klebsiella pneumoniae
  • Shigella

Frage 57

Frage
Name two Enterobacteriaceae that are major pathogens and state the disease they cause.
Antworten
  • Salmonella - Gastroenteritis, typhoid fever
  • Shigella - Bacterial dysentery
  • E. coli - Gastroenteritis, typhoid fever
  • Proteus vulgaris - Bacterial dysentery

Frage 58

Frage
Which commercial test systems are used to identify gram (-) rods in clinical labs?
Antworten
  • Entero tube
  • API
  • 20E
  • Micro ID
  • Micro tube
  • APE

Frage 59

Frage
Why do you use the streak-stab method for blood agar inoculations?
Antworten
  • So you can see the hemolysis better
  • To see if the bacteria like to grow in or on the agar

Frage 60

Frage
Where do you grow streptococcus and why?
Antworten
  • In a candle jar because it's a microaerophile
  • In the air because it's an aerobe

Frage 61

Frage
Which bacteria are alpha and optochin sensitive?
Antworten
  • Streptococcus pneumoniae
  • Streptococcus mitis
  • Streptococcus pyogenes
  • Group C Streptococcus
  • Enterococcis faecalis

Frage 62

Frage
Which bacteria are and optochin resistant?
Antworten
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Streptococcus pyogenes
  • Group C Streptococcus
  • Enterococcus faecalis

Frage 63

Frage
Which bacteria are beta and bacitracin sensitive?
Antworten
  • Streptococcus pyogenes
  • Streptococcus pneumoniae
  • Streptococcus mitis
  • Group C Streptococcus
  • Enterococcus faecalis

Frage 64

Frage
Which bacteria are beta and bacitracin resistant?
Antworten
  • Group C Streptococcus
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Enterococcus faecalis
  • Streptococcus pyogenes

Frage 65

Frage
How do you tell the difference between the alpha hemolytic Streptococcus and and Enterococcus faecalis?
Antworten
  • The alpha Streptococcus are bile esculin (-) and the Enterococcus faecalis is bile esculin (+)
  • The alpha Streptococcus are bile esculin (+) and the Enterococcus faecalis is bile esculin (-)

Frage 66

Frage
How does the catalase test differentiate between Staphylococcus and Streptococcus?
Antworten
  • Staphylococcus is catalase (+) and Streptococcus is catalase (-)
  • Staphylococcus is catalase (-) and Streptococcus is catalase (+)

Frage 67

Frage
What kind of hemolysis is shown on this plate?
Image:
3_Alpha_hemolysis_Strep_mitis_CNA (image/jpg)
Antworten
  • Alpha hemolyisis
  • Beta Hemolysis
  • Gamma Hemolysis

Frage 68

Frage
What is the mold pictured?
Image:
3a_Aspergillus_Septate_hyphae (image/jpg)
Antworten
  • Aspergillis
  • Penicillium
  • Rhizopus

Frage 69

Frage
What is the mold pictured?
Image:
3a_Aspergillus__10x__Lactophenol_cotton_blue (image/jpg)
Antworten
  • Aspergillus
  • Penicillium
  • Rhizopus

Frage 70

Frage
What is the mold pictured?
Image:
3a_Aspergillus__10x__Lactophenol_cotton_blue (image/jpg)
Antworten
  • Aspergillus
  • Penicillium
  • Rhizopus

Frage 71

Frage
What is the mold pictured?
Image:
3a_Aspergillus__400x (image/jpg)
Antworten
  • Aspergillus
  • Penicillium
  • Rhuzopus

Frage 72

Frage
What is the mold pictured?
Image:
3a_Aspergillus__400x (image/jpg)
Antworten
  • Aspergillus
  • Penicillium
  • Rhizopus

Frage 73

Frage
What is the mold pictured?
Image:
3a_Aspergillus_niger (image/jpg)
Antworten
  • Aspergillus
  • Penicillium
  • Rhizopus

Frage 74

Frage
What is the mold pictured?
Image:
3a_Penicillium_Culture (image/jpg)
Antworten
  • Aspergillus
  • Penicillium
  • Rhizopus

Frage 75

Frage
What mold is pictured?
Image:
3a_Fungi_Apergillus_slide_culture (image/jpg)
Antworten
  • Aspergillus
  • Penicillium
  • Rhizopus

Frage 76

Frage
What mold is pictured?
Image:
3a_Micro_Fungi_Micro_Fungi_032 (image/jpg)
Antworten
  • Aspergillus
  • Penicillium
  • Rhizopus

Frage 77

Frage
What mold is pictured?
Image:
3a_Penicillium_Slide_Culture (image/jpg)
Antworten
  • Aspergillus
  • Penicillium
  • Rhizopus

Frage 78

Frage
What mold is pictured?
Image:
3a_Penicillium__Lactophenol_cotton_blue__40x (image/jpg)
Antworten
  • Aspergillus
  • Penicillium
  • Rhizopus

Frage 79

Frage
What mold is pictured?
Image:
3a_Rhizopu_on_a_plate (image/jpg)
Antworten
  • Aspergillus
  • Penicillium
  • Rhizopus

Frage 80

Frage
What mold is pictured?
Image:
3a_Rhizopus_zygospore__40x (image/jpg)
Antworten
  • Aspergillus
  • Penicillium
  • Rhizopus

Frage 81

Frage
What mold is pictured?
Image:
3a_Rhizopus__40x (image/jpg)
Antworten
  • Aspergillus
  • Penicillium
  • Rhizopus

Frage 82

Frage
What mold is pictured?
Image:
3a_rhizopus (image/jpg)
Antworten
  • Aspergillus
  • Penicillium
  • Rhizopus

Frage 83

Frage
Is this result positive or negative for beta lactamase?
Image:
3aBeta_lactamase_test_positive_A (image/jpg)
Antworten
  • Positive
  • Negative

Frage 84

Frage
Is this result positive or negative for beta lactamase?
Image:
3a_Beta_lactamase_test_negative_A (image/jpg)
Antworten
  • Positive
  • Negative

Frage 85

Frage
What are the results of the bile esculin test pictured?
Image:
3_Bile (image/jpg)
Antworten
  • A = negative, B = positive
  • A = positive, B = negative

Frage 86

Frage
What are the results of the carbohydrate fermentation pictured?
Image:
3_Carbohydrate_fermentation.JPG (image/JPG)
Antworten
  • A = negative, B = positive, C = positive + gas
  • A = positive, B = negative, C = negative +gas

Frage 87

Frage
What are the results of the citrate test pictured?
Image:
3_Citrate.JPG (image/JPG)
Antworten
  • A = negative, B = positive
  • A = positive, B = negative

Frage 88

Frage
What are the results of the indole test pictured?
Image:
3_INDOLE.JPG (image/JPG)
Antworten
  • A = negative, B = positive
  • A = positive, B = negative

Frage 89

Frage
What is the result of the lactose fermentation pictured?
Image:
3_macconkey (image/jpg)
Antworten
  • A = negative, B = positive, C = positive
  • A = negative, B = negative, C = positive
  • A = positive, B = negative, C = negative
  • A = positive, B = positive, C = negative

Frage 90

Frage
What are the results of the motility test pictured?
Image:
3_motilitylabeled (image/jpg)
Antworten
  • A = non-motile, B = motile, C = motile
  • A = non-motile, B = non-motile, C = motile
  • A = motile, B =motile, C = non-motile
  • A = motile, B = non-motile, C = non-motile

Frage 91

Frage
What are the results of the sensitivity test pictured?
Image:
3_opto_srpn-srvi (image/jpg)
Antworten
  • A = sensitive, B = resistant
  • A = resistant, B = sensitive

Frage 92

Frage
How do you calculate the titer of phage in the original sample given the number of plaques and the dilution of the plate?
Antworten
  • # of plaques x dilution x 10^-1 = _________ PFU/mL
  • # of plaques x dilution x 10^1= __________ PFU/mL

Frage 93

Frage
What kind of hemolysis is pictured?
Image:
3_Gamma_hemolysis_M_luteus_Blood_Agar (image/jpg)
Antworten
  • Gamma
  • Alpha
  • Beta

Frage 94

Frage
Which bacteria is cogulase positive and manitol slat agar positive?
Antworten
  • Staphylococcus aureus
  • Staphylococcus epiderminis
  • Micrococcus roseus
  • Micrococcus luteus

Frage 95

Frage
Which bacteria is coagulase negative and manitol salt agar negative?
Antworten
  • Staphylococcus epidermidis
  • Stapylococcus aureus
  • Micrococcus roseus
  • Micrococcus luteus

Frage 96

Frage
What bacteria is positive for nitrate reduction and rose for pigment production?
Antworten
  • Micrococcus roseus
  • Micrococcus luteus
  • Staphylococcus aureus
  • Staphylococcus epidermidis

Frage 97

Frage
What bacteria is negative for nitrate reduction and yellow for pigment production?
Antworten
  • Micrococcus luteus
  • Micrococcus roseus
  • Staphylococcus aureus
  • Staphylococcus epidermidis

Frage 98

Frage
How is Enterococcus faecalis differentiated from other alpha hemolytic streptococcus?
Antworten
  • It is positive for bile esculin
  • It is negative for bile esculin

Frage 99

Frage
Which Streptococcus bacteria is optochin resistant?
Antworten
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Streptococcus pyogenes
  • Group C streptococcus

Frage 100

Frage
Which streptococcus bacteria is optochin sensitive?
Antworten
  • Streptococcus pneumoniae
  • Streptococcus mitis
  • Streptococcus pyogenes
  • Group C Streptococcus

Frage 101

Frage
Which bacteria is bacitration resistant?
Antworten
  • Group C Streptococcus
  • Streptococcus pyogenes
  • Streptococcus pneumoniae
  • Streptococcus mitis

Frage 102

Frage
Which bacteria is bacitracin sensitive?
Antworten
  • Streptococcus pyogenes
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Group C Streptococcus

Frage 103

Frage
Which bacteria are alpha hemolytic?
Antworten
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Streptococcus pyogenes
  • Group C Streptococcus

Frage 104

Frage
Which bacteria are beta hemolytic?
Antworten
  • Streptococcus mitis
  • Streptococcus pneumoniae
  • Streptococcus pyogenes
  • Group C Streptococcus

Frage 105

Frage
What hemolysis is pictured?
Image:
Staph_hemolysis_Blood_Agar (image/jpg)
Antworten
  • A= alpha, B = beta, C = gamma
  • A = beta, B = alpha, C = gamma
  • A = gamma, B = alpha, C = beta
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