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Erstellt von gina_evans0312
vor mehr als 10 Jahre
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Frage | Antworten |
Marker | Loci/Mendelian characteristic that can be traced through a pedigree |
Role of a marker | Act as a reference point in linkage studies |
SNP | Small Nucleotide Polymorphism- small code variations between individuals |
Polymorphism | Many alleles |
More polymorphic = | More useful, as individuals are more likely to be heterozygotes |
If recombinants between an SNP & a gene are uncommon... | The two are mapped close together |
RFLP | Restriction Fragment Length Polymorphism |
RFLP relies on... | One allele having a Polymorphic Restriction Site |
DNA probe | Done to find Polymorphic Restriction Site |
Restriction Enzyme | Used to cut at Polymorphic Restriction Site to create multiple pieces |
Detection of RFLP | Run a Southern Blot or Gel Electrophoresis for genetic material |
Homozygosity in RFLP | 1 line on Southern Blot/Gel |
SSLP/Microsatellites | Single Sequence Length Polymorphism |
Detecting Microsatellites/SSLP's | PCR amplification using markers long around M.S |
Microsatellite Primers | Fluorescent, 1-4bp long |
Using Gel Eletrophoresis | Use PCR before cutting to ensure enough material |
Image:
SSLP (image/png)
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SSLP/Microsatellites |
Image:
SSLP_Analysis (image/png)
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A is homozygous, B is heterozygous |
SNP | Single Nucleotide Polymorphism- areas that differ by a single base, even in highly conserved areas |
Oligonucleotide Hybridisation | Used for detecting SNP's- very specific |
Detecting SNP's- Fluorescent Tag | Released when oligonucleotide probe binds to the DNA |
Detecting SNP's- Quencher Tag | Prevents Fluorescent tag from Fluorescing unless hybrid has been formed |
Detecting SNP's- Hybrids | Once oligonucleotide has bound, base pairing determines whether binding is stable or unstable |
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