Markers

Frage	Antworten
Marker	Loci/Mendelian characteristic that can be traced through a pedigree
Role of a marker	Act as a reference point in linkage studies
SNP	Small Nucleotide Polymorphism- small code variations between individuals
Polymorphism	Many alleles
More polymorphic =	More useful, as individuals are more likely to be heterozygotes
If recombinants between an SNP & a gene are uncommon...	The two are mapped close together
RFLP	Restriction Fragment Length Polymorphism
RFLP relies on...	One allele having a Polymorphic Restriction Site
DNA probe	Done to find Polymorphic Restriction Site
Restriction Enzyme	Used to cut at Polymorphic Restriction Site to create multiple pieces
Detection of RFLP	Run a Southern Blot or Gel Electrophoresis for genetic material
Homozygosity in RFLP	1 line on Southern Blot/Gel
SSLP/Microsatellites	Single Sequence Length Polymorphism
Detecting Microsatellites/SSLP's	PCR amplification using markers long around M.S
Microsatellite Primers	Fluorescent, 1-4bp long
Using Gel Eletrophoresis	Use PCR before cutting to ensure enough material
Image: SSLP (image/png)	SSLP/Microsatellites
Image: SSLP_Analysis (image/png)	A is homozygous, B is heterozygous
SNP	Single Nucleotide Polymorphism- areas that differ by a single base, even in highly conserved areas
Oligonucleotide Hybridisation	Used for detecting SNP's- very specific
Detecting SNP's- Fluorescent Tag	Released when oligonucleotide probe binds to the DNA
Detecting SNP's- Quencher Tag	Prevents Fluorescent tag from Fluorescing unless hybrid has been formed
Detecting SNP's- Hybrids	Once oligonucleotide has bound, base pairing determines whether binding is stable or unstable

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