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11814527
Disaggregation of Explant
Description
Flowchart on Disaggregation of Explant, created by vallarasu VîP on 27/12/2017.
Flowchart by
vallarasu VîP
, updated more than 1 year ago
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Created by
vallarasu VîP
over 6 years ago
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Resource summary
Flowchart nodes
DISAGGREGATION OF EXPLANTS
Primary explant technique
Mechanical diasaggregation
Dissection of explant
Finely chopped
Washed by setting
Transfer in last wash
REmove BSS
Add thin flim of medium
Explant out growth
Incubate 2 to 3 weeks
TISSUE SAMPLE
CELL+DEBRIS
MORE CELLS+DEBRIS
VIABEL CELLS+DEBRIS
DILUTE THE CELLS
INCUBATE
Enzymatic disaggregation
Biopsy in medium+antibiotic
Dissection in BSS+antibiotic
Chopped tissue
Complete medium+ collagenase
Dissagregated tissue+ tissue residues
Dispersed cells+residual culture of epithelial cells
Dispersed cell suspension contains enriched fibrobiasts
Enriched epithelial fraction
Primary cell culture
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