diaggregation of explants

Flowchart nodes

• Selection and isolation of organ

• Dissection and removal of necrotic cells and fat cells

• Mechanical dissagregation

• Enzymatic dissagregation

• Primary expant technique

• Chopping and slicing of tissue, sieving, syringing, pippeting, etc.

• Trypsin 

• Collagenase

• Other enzymes ( bacterial proteases)

• Explant

• Warm Trypsinization

• Cold Trypsinization

• Resuspended and seed

• Primary culture 

• sub-culture 

• Cell line