Almost complete 16s rRNA gene
sequence aligned manually using
MEGA version 5 software
Phylogenetic and bootrap were inferred
Further evaluated by Fatty Acid
Methyl ester
Peak were identified by Sherlock
Microbial Identification (MIDI)
Inoculating selected isolates
to tryptic soy broth
With wheat bran and
incubated at 20 degree
celcius for 1 day
Enzyme was sampled for
centrifugation & Filtration
Enzyme extracted and incubated at
20 degree celcius for 30 min
Cellulase activity was also assayed
using Dixtrin, microcrystallline cellulose,
and xylan as subtrates
Reaction interrupted with
addition of 3,5-dinitrosalicyclic
acid and heated at 100 degree
celcius for 5 min prior to
quantification by
spectrophotometer at 540 nm
TSB version 6 database
7683 Series injector, Flame
ionization detector, HP-5
capillary column