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Creating an E.Coli Library

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Year 2 Quiz on Creating an E.Coli Library, created by gina_evans0312 on 29/12/2013.
gina_evans0312
Quiz by gina_evans0312, updated more than 1 year ago
gina_evans0312
Created by gina_evans0312 almost 11 years ago
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Resource summary

Question 1

Question
Electroporations- passing electricity through a mix of DNA and bacteria causes DNA to be taken up
Answer
  • True
  • False

Question 2

Question
What does N stand for?
Image:
Library_Size (image/png)
Answer
  • The no of clones needed
  • P of getting a clone with the desired gene
  • Genome size

Question 3

Question
What are the meanings of i and g?
Image:
Library_Size (image/png)
Answer
  • i = Library size g= probability of getting desired gene
  • i = avg insert size g = genome size
  • i = probability of getting desired gene g = genome size

Question 4

Question
The larger the genome, the larger the library required to replicate it
Answer
  • True
  • False

Question 5

Question
When is cDNA used?
Answer
  • When an organism has a lot of non-coding DNA
  • When an organism is very small
  • When the DNA is degraded

Question 6

Question
How is cDNA made?
Answer
  • Converted from mRNA
  • Converted from snoRNA
  • Converted from siRNA

Question 7

Question
Less than 1% of the genome is mRNA, but it's exclusively expressible genes
Answer
  • True
  • False

Question 8

Question
What is required to make the first RNA-DNA hybrid?
Image:
Hybrid (image/png)
Answer
  • dNTP's
  • Primer
  • Reverse Transcriptase

Question 9

Question
What are the two methods of degrading the RNA part?
Answer
  • Alkylisation (RNA is less stable)
  • RNA digestion through RNAase H
  • Boiling
  • Restriction enzymes

Question 10

Question
Which method was used to get the following DNA strand?
Image:
Alkali_Degrading (image/png)
Answer
  • Alkali degrading
  • Boiling
  • Shaking
  • RNAase H

Question 11

Question
What is required to get from the former to the latter?
Image:
Alkali_Degrading (image/png)
Answer
  • dNTP's
  • DNA Polymerase
  • Primer
  • Reverse transcriptase

Question 12

Question
What method was used to degrade the RNA into the following?
Image:
RNAase_H (image/png)
Answer
  • RNAase H
  • Alkylation
  • Boiling
  • Sonication

Question 13

Question
What is needed to get from the former to the latter?
Image:
Final_Product (image/png)
Answer
  • dNTP's
  • DNA Polymerase H
  • RNAase
  • rNTP's

Question 14

Question
When creating cDNA, a little information is always lost from the 3' end
Answer
  • True
  • False

Question 15

Question
How do we prevent this loss of information?
Answer
  • After degrading the RNA, add a homopolymer (polyC) tail to the 3 end
  • Using Terminal Transferase
  • Before degrading the DNA, degrade the Poly A tail

Question 16

Question
Then in second strand synthesis add ...
Answer
  • dNTP's
  • DNA polymerase
  • Oligo-dG primer

Question 17

Question
Enriching desired DNA means creating/finding more of it
Answer
  • True
  • False

Question 18

Question
Selective Hybridisation removes cDNA's common to more than one tissue
Answer
  • True
  • False

Question 19

Question
Tissue Specificity is useful if the gene is only expressed in a certain tissue
Answer
  • True
  • False

Question 20

Question
What is Normalisation?
Answer
  • Melt, anneal and isolate ssDNA
  • Use restriction enzymes to repeatdely cut and ligate DNA
  • Use DNA helicase to continually replicate DNA

Question 21

Question
How does Normalisation work?
Answer
  • Repetitive DNA binds to other repetitive DNA
  • Leaving only unique DNA single stranded
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