Wk 7 - Applied Microbiology and Haemotology

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Wk 7 - Applied Microbiology and Haemotology MEDI1000
Jessica Bulley
Test por Jessica Bulley, actualizado hace más de 1 año
Jessica Bulley
Creado por Jessica Bulley hace más de 6 años
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Resumen del Recurso

Pregunta 1

Pregunta
Select two reasons for preventing microbes from getting into food.
Respuesta
  • Prevent slow decomposition process
  • Prevent bad odors
  • Prevent food spoilage
  • Prevent food related illnesses

Pregunta 2

Pregunta
Select Five ways how could microbes contaminate food
Respuesta
  • • with soil
  • • preparation procedures
  • • improper storage
  • • harvesting/slaughtering
  • • handling
  • • incorrect food stacking in fridge
  • • delayed pesticides

Pregunta 3

Pregunta
How does fermentation preserve food?
Respuesta
  • Increases shelf life and safety of product and Improves digestibility, nutritional content
  • Increases putrification and safety of product and speeds up disease process, nutritional content

Pregunta 4

Pregunta
List Five relevant boxes to pathogenic bacteria, viruses and parasites that can cause illness through food or water
Respuesta
  • Salmonella spp. infection Vibrio cholera Vibrio parahaemolyticus
  • Rotavirus Hepatitis A Polio Vaccine Adenovirus
  • Helminths Crytosporidium parvum Entamaeba histolytica Giardia lamblia
  • Bacillus cereus intoxication Clostridium botulinum Staphylococcus aureus Campylobacter infection
  • Norovirus Astrovirus Entrovirus (Echovirus)
  • Rhinovirus Sinusitus Pleurusy TSS

Pregunta 5

Pregunta
Norovirus, Astrovirus and Entrovirus (Echovirus) are types of parasitic foodborne diseases
Respuesta
  • True
  • False

Pregunta 6

Pregunta
Bacillus cereus, Clostridium botulinum, Staphylococcus aureus, Campylobacter infection, Salmonella spp. Vibrio cholera & Vibrio parahaemolyticus are types of Bacterial foodborne diseases
Respuesta
  • True
  • False

Pregunta 7

Pregunta
Why does spoilage of food appear rapid when the microbes are on the food for long periods before the appearance of spoilage?
Respuesta
  • • Due to exponential growth pattern microbes
  • • Due to stationary pattern of microbes

Pregunta 8

Pregunta
List Two uses for microbes in the industry.
Respuesta
  • Genetic engineering – deliberate modification of an organisms genome
  • Recombinant DNA technology – isolating a specific gene and inserting into a vector (plasmid) to form a recombinant molecule and producing large amounts of gene product
  • High transfection efficiency

Pregunta 9

Pregunta
List Five reasons for treating waste water.
Respuesta
  • • Remove pathogens: bacteria, viruses, eggs and cysts of parasitic worms
  • • Remove nutrients that cause pollution
  • • Prevent foul odours: H2S, amines, oil and grease scum
  • • Prevent contamination of drinking water
  • • Remove toxins: metals, pesticides, herbicides
  • • Remove bad taste

Pregunta 10

Pregunta
What is B.O.D?
Respuesta
  • Biochemical Oxygen Demand
  • Biological Oxydation Determination

Pregunta 11

Pregunta
Why is Biochemical Oxygen Demand important?
Respuesta
  • Determines the levels of biological degradable organic material present in water
  • Determine the levels of biological mercury not organic material present in water

Pregunta 12

Pregunta
What is the main process in secondary sewage treatment?
Respuesta
  • Microbial digestion of organic material and hydrolysis
  • Microbial digestion of organic material and Haemolysis

Pregunta 13

Pregunta
Give 3 uses for Bacteriophage
Respuesta
  • • Possible treatment for bacterial infection
  • • Identify pathogenic bacteria (Phage typing)
  • • Recombinant DNA technology
  • • Complete eradication of Viruses

Pregunta 14

Pregunta
What are the advantages of using HIV as a vector for gene therapy?
Respuesta
  • • Retrovirus • Reverse transcriptase generates DS-DNA • Integrated into host chromosome • Gene replacement therapy • Deliver and express gene
  • • Rhinovirus • Quickly transcriptase generates DS-DNA • Non-conforming into host chromosome • Prevention of Gene replacement therapy • Deliver and express gene

Pregunta 15

Pregunta
Name 5 different areas of study within the field of Haematology
Respuesta
  • Haemopoiesis
  • Coagulation
  • Haemoglobin
  • Formed elements of blood
  • Blood transfusion / cross matching
  • Dialylsis

Pregunta 16

Pregunta
What is Anaemia?
Respuesta
  • An abnormal decrease of RBC
  • An abnormal increase of RBC

Pregunta 17

Pregunta
Explain what is meant by the term Leukaemia.
Respuesta
  • An abnormal increase of WBC
  • An abnormal decrease of WBC

Pregunta 18

Pregunta
What is Haemopoiesis?
Respuesta
  • The production of the formed elements of blood - WBC’s, RBC’s and platelets
  • The breakdown of the formed elements of blood - WBC’s, RBC’s and platelets

Pregunta 19

Pregunta
What is Erythropoiesis?
Respuesta
  • The process which produces red blood cells (erythrocytes).
  • The process which produces white blood cells (leukocytes).

Pregunta 20

Pregunta
What is the correct term for a decrease in neutrophils?
Respuesta
  • Neutropaenia
  • Neutrophilia

Pregunta 21

Pregunta
Where does Haemopoiesis occur? Select Two answers
Respuesta
  • Foetus - yolk sac, liver, spleen, bone marrow, lymph nodes
  • Adult - bone marrow
  • Foetus - liver, kidneys, placenta, bone marrow, lymph nodes
  • Adult - bone marrow, liver

Pregunta 22

Pregunta
Describe the shape of a RBC. Why do RBCs have this shape? Select Two answers
Respuesta
  • Shape allows for slow and constant diffusion of gasses, flexibility, change in shape/size
  • Biconcave disc - sturdy
  • Biconcave disc - flexible
  • Shape allows for rapid diffusion of gasses, flexibility, change in shape/size

Pregunta 23

Pregunta
Discuss the significance of the RBC membrane.
Respuesta
  • rapid diffusion of gasses
  • slow yet constant diffusion of gasses

Pregunta 24

Pregunta
List 3 Granulocytes that can be found in peripheral blood.
Respuesta
  • Neutrophils
  • Basophils
  • Eosinophils
  • Monocytes
  • Lymphocytes

Pregunta 25

Pregunta
List each of the 5 WBC types found in peripheral blood
Respuesta
  • Neutrophils
  • Monocytes
  • Lymphocytes
  • Eosinophils
  • Basophils
  • Leukocytes
  • Platelets

Pregunta 26

Pregunta
Neutrophils are the most common type of WBC and have
Respuesta
  • small granules in cytoplasm
  • large granules in cytoplasm

Pregunta 27

Pregunta
Eosinophils have
Respuesta
  • large strongly staining (orange/red) cytoplasm granules
  • small strongly staining (blue/purple) cytoplasm granules

Pregunta 28

Pregunta
Basophils have
Respuesta
  • large cytoplasmic granules (dark staining)
  • small cytoplasmic granules (orange staining)

Pregunta 29

Pregunta
Lymphocytes have a
Respuesta
  • large round nucleus/less cytoplasm
  • no nucleus/lots of cytoplasm

Pregunta 30

Pregunta
Monocytes are the largest WBC and have a
Respuesta
  • kidney shaped nucleus
  • liver shaped nucleus

Pregunta 31

Pregunta
Select type of WBC
Respuesta
  • Neutrophils
  • Eosinophils

Pregunta 32

Pregunta
Select type of WBC
Respuesta
  • Eosinophils
  • Basophils

Pregunta 33

Pregunta
Select type of WBC
Respuesta
  • Basophils
  • Neutrophils

Pregunta 34

Pregunta
Select type of WBC
Respuesta
  • Lymphocytes
  • Basophils

Pregunta 35

Pregunta
Select type of WBC
Respuesta
  • Monocytes
  • Eosinophils

Pregunta 36

Pregunta
What is the term used to describe variation in RBS size?
Respuesta
  • Anisocytosis
  • Poikilocytosis

Pregunta 37

Pregunta
Select the correct definition of Elliptocytes (RBC Poikilocytosis)
Respuesta
  • – Oval shaped rather than thin or round – Less pronounced defects – Found in some types of anaemia
  • – Thin elongated cigar shaped cells – Change to membrane structure – Found in various types of anaemia

Pregunta 38

Pregunta
Select the correct definition of Ovalocytes (RBC Poikilocytosis)
Respuesta
  • – Oval shaped rather than thin or round – Less pronounced defects – Found in some types of anaemia
  • – Thin elongated cigar shaped cells – Change to membrane structure – Found in various types of anaemia

Pregunta 39

Pregunta
Select the correct definition of Codocytes (RBC Poikilocytosis)
Respuesta
  • – Loss of biconcave shape – Increased surface area:volume ratio
  • – Loss of biconcave shape - sphere – Decreased surface area:volume ratio – Found in many anaemias

Pregunta 40

Pregunta
Select the correct definition of Spherocytes (RBC Poikilocytosis)
Respuesta
  • – Loss of biconcave shape - sphere – Decreased surface area:volume ratio – Found in many anaemias
  • – Loss of biconcave shape – Increased surface area:volume ratio

Pregunta 41

Pregunta
Select the correct definition of Schistocytes (RBC Poikilocytosis)
Respuesta
  • – Irregularly contracted fragments – Variable appearance – Found in conditions with trauma to RBC’s e.g. burns
  • – Elongated, crescent shaped – Sickle cell anaemia – Abnormal haemoglobin (HbS)

Pregunta 42

Pregunta
Select the correct definition of Drepanocytes (RBC Poikilocytosis)
Respuesta
  • – Elongated, crescent shaped – Sickle cell anaemia – Abnormal haemoglobin (HbS)
  • – Irregularly contracted fragments – Variable appearance – Found in conditions with trauma to RBC’s e.g. burns

Pregunta 43

Pregunta
Select the correct definition of Acanthocytes (burr cells) (RBC Poikilocytosis)
Respuesta
  • – Multiple irregular thorny projections – Changes in phospholipid metabolism – Found in liver disease
  • – Teardrop shape – Stretched out of shape - excessive time (spleen) – Found in many conditions

Pregunta 44

Pregunta
Select the correct definition of Dacrocytes (teardrop cells) (RBC Poikilocytosis)
Respuesta
  • – Teardrop shape – Stretched out of shape - excessive time (spleen) – Found in many conditions
  • – Multiple irregular thorny projections – Changes in phospholipid metabolism – Found in liver disease

Pregunta 45

Pregunta
Select the correct definition of Stomatocytes (RBC Poikilocytosis)
Respuesta
  • - Oval or rectangular area of central pallor – “Cup” - shaped – Found in liver disease
  • – Short, evenly spaced pointed projections – Acute blood loss, burns, uraemia - kidney failure

Pregunta 46

Pregunta
Select the correct definition of Echinocytes (RBC Poikilocytosis)
Respuesta
  • – Short, evenly spaced pointed projections – Acute blood loss, burns, uraemia - kidney failure
  • – Oval or rectangular area of central pallor – “Cup” - shaped – Found in liver disease

Pregunta 47

Pregunta
What is Polychromasia and what may it indicate?
Respuesta
  • Abnormal coloured RBC's (blue, orange) which may indicate increased number of immature RBC’s
  • Abnormal pale RBC's which may indicate decreased number of immature RBC’s

Pregunta 48

Pregunta
What physiological processes do platelets have a role in?
Respuesta
  • Primary role is the prevention of blood loss
  • Primary role is the encouragement of blood loss

Pregunta 49

Pregunta
What does the term “Thrombocytopaenia” mean?
Respuesta
  • Decreased platelet count
  • Increased platelet count

Pregunta 50

Pregunta
What is the term for an increased number of Lymphocytes in the peripheral blood?
Respuesta
  • Lymphocytosis
  • Lymphopaenia

Pregunta 51

Pregunta
Either of 2 principles can be used in Automated Cell Counting machines. What are these 2 principles?
Respuesta
  • Manual or automated
  • Direct or indirect

Pregunta 52

Pregunta
What blood cells can be manually counted using a Haemocytometer? (Can the chamber also be used to count anything else besides blood cells?)
Respuesta
  • All blood cells. The chamber can also be used to count bacterial cells in suspension and WBC’s in CSF
  • All blood cells aside from platelets. The chamber can also be used to count bacterial cells in suspension and WBC’s in CSF

Pregunta 53

Pregunta
Different areas of a counting chamber are used for counting different types of blood cells. What areas are used for a manual WCC?
Respuesta
  • The Diff
  • Relative count

Pregunta 54

Pregunta
Explain why WBC types should always be reported as Absolute Number and not Relative numbers following a Diff.
Respuesta
  • Absolute Number count indicates the exact number of cells per RBC/WBC which may differ from other relative levels of different cells.
  • Due to possible computer malfunctions, it is possible to have an incorrect relative number statement of sample.

Pregunta 55

Pregunta
How do autoimmune diseases occur?
Respuesta
  • An autoimmune disorder occurs when the body's immune system attacks and destroys healthy body tissue by mistake.
  • An autoimmune disorder occurs when the body's digestive system attacks and destroys healthy body tissue by mistake.

Pregunta 56

Pregunta
Describe the difference between passive and active vaccination.
Respuesta
  • Passive immunity is provided when a person is given antibodies to a disease rather than producing them through his or her own immune system. Active immunity results when exposure to a disease organism triggers the immune system to produce antibodies to that disease.
  • Passive immunity results when exposure to a disease organism triggers the immune system to produce antibodies to that disease. Active immunity is provided when a person is given antibodies to a disease rather than producing them through his or her own immune system.

Pregunta 57

Pregunta
What does MHC stand for?
Respuesta
  • Major histocompatibility complex
  • Major histopathology complex

Pregunta 58

Pregunta
What is the purpose of MHC?
Respuesta
  • The function of MHC molecules is to bind peptide fragments derived from pathogens and display them on the cell surface for recognition by the appropriate T cells.
  • The function of MHC molecules is to bind T cell fragments derived from pathogens and display them on the cell surface for recognition by the appropriate T cells.

Pregunta 59

Pregunta
Adaptive immunity refers to antigen-specific immune response and Innate immunity refers to nonspecific defense mechanisms
Respuesta
  • True
  • False

Pregunta 60

Pregunta
Innate immunity refers to antigen-specific immune response and Adaptive immunity refers to nonspecific defense mechanisms
Respuesta
  • True
  • False

Pregunta 61

Pregunta
....refers to antigen-specific immune response. The adaptive immune response is more complex than the innate. The antigen first must be processed and recognized. Once an antigen has been recognized, the adaptive immune system creates an army of immune cells specifically designed to attack that antigen. Adaptive immunity also includes a "memory" that makes future responses against a specific antigen more efficient.
Respuesta
  • Adaptive immunity
  • Innate immunity

Pregunta 62

Pregunta
B Cells ....
Respuesta
  • .....proliferate into Memory B cells and plasma cells
  • ..... do not proliferate into Memory B cells and plasma cells

Pregunta 63

Pregunta
Memory B cells are derived from B cells. They are immune specific towards a certain bacterium or virus.
Respuesta
  • True
  • False

Pregunta 64

Pregunta
Plasma cells are derived from B cells. They enlarge and secrete large amounts of antibodies.
Respuesta
  • True
  • False

Pregunta 65

Pregunta
The Humoral response: [blank_start]4.[blank_end] B cells produce memory cells. Memory cells provide future immunity. [blank_start]1.[blank_end] Antigens bind to B cells. [blank_start]3.[blank_end] B cells proliferate and produce plasma cells. The plasma cells bear antibodies with the identical antigen specificity as the antigen receptors of the activated B cells. The antibodies are released and circulate through the body, binding to antigens. [blank_start]2.[blank_end] Interleukins or helper T cells costimulate B cells. In most cases, both an antigen and a costimulator are required to activate a B cell and initiate B cell proliferation.
Respuesta
  • 4.
  • 1.
  • 3.
  • 2.

Pregunta 66

Pregunta
A T cell, or T lymphocyte, is a type of lymphocyte (a subtype of white blood cell) that plays a central role in cell-mediated immunity.
Respuesta
  • True
  • False

Pregunta 67

Pregunta
When a Macrophage engulfs a Pathogen, it becomes a
Respuesta
  • Antigen Presenting Cell
  • Antibiotic Presenting Cell

Pregunta 68

Pregunta
Helper T cells recognize Antigen Presenting Cells and helps to stimulate B cells to divide into Plasma cells
Respuesta
  • True
  • False

Pregunta 69

Pregunta
Helper B cells recognize Antigen Presenting Cells and helps to stimulate B cells to divide into Plasma cells
Respuesta
  • True
  • False

Pregunta 70

Pregunta
Memory helper T cells are a part of the Memory bank which help to attack and destroy the same pathogen if it was to return again.
Respuesta
  • True
  • False

Pregunta 71

Pregunta
[blank_start]T[blank_end] Cells are produced in the [blank_start]Thymus[blank_end], once they have matured they are sent to the [blank_start]Spleen[blank_end] or to circulate around the [blank_start]Lymph[blank_end] system.
Respuesta
  • T
  • B
  • Thymus
  • Bone marrow
  • Spleen
  • Sternum
  • Lymph
  • Cardiovascular

Pregunta 72

Pregunta
A Cytotoxic T cell (killer T cell) is a type of immune cell that can kill certain cells, including foreign cells, cancer cells, and cells infected with a virus.
Respuesta
  • True
  • False

Pregunta 73

Pregunta
A cell‐mediated response involves mostly T cells and responds to any cell that displays aberrant MHC markers, including cells invaded by pathogens, tumor cells, or transplanted cells.
Respuesta
  • True
  • False

Pregunta 74

Pregunta
The cell‐mediated response involves mostly [blank_start]T[blank_end] cells
Respuesta
  • T
  • B

Pregunta 75

Pregunta
The humoral response (or antibody‐mediated response) involves mainly [blank_start]B[blank_end] cells
Respuesta
  • B
  • T

Pregunta 76

Pregunta
Select the different types of T cells.
Respuesta
  • Killer T-cells
  • Helper T-cells
  • Macrophage
  • Bank T Cells

Pregunta 77

Pregunta
Poikilocytes are abnormally shaped red blood cells
Respuesta
  • True
  • False
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