450891
Descripción
Test por gina_evans0312, actualizado hace más de 1 año
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Creado por gina_evans0312
hace más de 10 años
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Pregunta 1
Pregunta
Which of the following is not a role of the DNAK/Hsp70 superfamily?
Respuesta
-
Stabilise nascent polypeptides
-
Repaor heat shocked proteins
-
Preserve folding proteins
-
Protein transport
-
Protein degredation
Pregunta 2
Pregunta
Hsp70s (unlike DNAK's) do not require chaperones
Respuesta
- True
- False
Pregunta 3
Pregunta
What is the role of DNAJ/Hsp40 when combined with DNAK/Hsp70
Respuesta
-
ATPases
-
Kinases
-
Peptidyl propyl isomerases
Pregunta 4
Pregunta
DNAK/Hsp70 need NEF's in order to function
Respuesta
- True
- False
Pregunta 5
Pregunta
Where is the Hsp70/DNAK binding domain for ATP?
Respuesta
-
N-terminus
-
Core
-
C- terminus
Pregunta 6
Pregunta
Name the subdomains of the ATPase found in DNAK/Hsp70
Respuesta
-
1A
-
1B
-
1C
-
2A
-
2B
-
2C
Pregunta 7
Pregunta
What increases the ATPase activity of the ATP binding domain?
Respuesta
-
The binding of the substrate
-
Movement into an acidic compartment
-
DNAJ/Hsp40 binding
-
YDJ1 binding
-
SSA
Pregunta 8
Pregunta
The core substrate binding domain has an affinity for what?
Respuesta
-
Neutral, hydrophobic aa
-
Acidic, polar aa
-
Basic, polar aa
Pregunta 9
Pregunta
The C-terminus of DNAK/Hsp 70 acts as a 'lid' for the protein
Respuesta
- True
- False
Pregunta 10
Pregunta
When ATP is hydrolysed in DNAK/Hsp70, where does the C-terminus bind to?
Respuesta
-
The N-terminus
-
The core
-
It doesn't move at all
-
Over the bound substrate
Pregunta 11
Pregunta
Without the assistance of DNAJ/Hsp40, the ATPase in DNAK/Hsp70 works too slowly for the C-terminus to trap the bound substrate
Respuesta
- True
- False
Pregunta 12
Pregunta
Hsp70/DNAK's preferred binding pattern is 15 aa long
Respuesta
- True
- False
Pregunta 13
Pregunta
What are the 'Flanking' residues (1-4, 9-13) of Hsp70's preferred binding site made of?
Respuesta
-
Leu
-
Arg
-
Lys
-
Trp
Pregunta 14
Pregunta
The preferred residue for the -Core- of Hsp70's preferred binding site is Valine
Respuesta
- True
- False
Pregunta 15
Pregunta
What other residues can make up the 'core' of the preferred binding site/
Respuesta
-
Ile
-
Val
-
Phe
-
Tyr
Pregunta 16
Pregunta
How does this 'Preferred Binding Patter' for Hsp70 help direct the protein?
Respuesta
-
In properly folded proteins, the hydrophobic stretch is hidden, and so only nascent/denatured proteins have the exposed residues for Hsp70 to bind to
-
In nascent/denatured proteins, these hydrophobic residues will be added by Hsp40, so it can recognise them
-
Stretches with the core residues are removed during protein folding, so only nascent polypeptides have them
Pregunta 17
Pregunta
What is the role of the J domain in a Hsp40?
Respuesta
-
Contain the HPD peptide needed to boost Hsp40's ATPase
-
Gly/Phe rich linker for positioning
-
Dimerisation domain
-
Zn+ 'finger' for substrate binding
Pregunta 18
Pregunta
The role of the Gly/Phe rich linker does what?
Respuesta
-
Makes DNAJ/Hsp40 flexible enough to bind to its respective protein
-
Makes DNAJ/Hsp40 a more effective ATPase
-
Allows DNAJ/Hsp40 to locate its respective DNAK/Hsp70
Pregunta 19
Pregunta
The Zinc 'Finger' is for substrate binding
Respuesta
- True
- False
Pregunta 20
Pregunta
Which amino acids make up the HPD domain?
Respuesta
-
Histadine
-
Proline
-
Aspartate
-
Leucine
-
Glycine
Pregunta 21
Pregunta
The Znc finger of DNAJ/Hsp40 can interact with the nascent polypeptide and bring it to the DNAK/Hsp70
Respuesta
- True
- False
Pregunta 22
Pregunta
What is the sequence of the Zinc 'finger'?
Respuesta
-
CXXCXGXG
-
GXXGXCXC
-
GXXCXGXC
-
CXXGXCXG
Pregunta 23
Pregunta
The C-terminus of Hsp40 is for tetramerisation
Respuesta
- True
- False
Pregunta 24
Pregunta
What effect does the GEF factor binding have on the Hsp70/Hsp40 complex?
Respuesta
-
ADP is replaced with ATP
-
ATP is replaced with ADP
-
Hsp40 dissociates
-
Newly folded protein binds
-
Newly folded protein dissociates
Pregunta 25
Pregunta
When the substrate & Hsp40 bind to Hsp70, the GEF factor also binds
Respuesta
- True
- False
Pregunta 26
Pregunta
How is the nascent polypeptide transferred from the DNAJ site to the DNK site?
Respuesta
-
The G/F domain is flexible enough to bind to the DNAK site and pull it in for transfer
-
The J domain is pulled like a thread through the ATPase, bringing the two sites close enough together for transfer
-
The dimerisation of the DNAJ pulls the two sites close together
Pregunta 27
Pregunta
In which of the following is Prefoldin found?
Respuesta
-
Archea
-
Prokaryotes
-
Eukaryotes
Pregunta 28
Pregunta
Prefoldin can act as a substitute for Hsp70 if the organsim doesn't have the latter
Respuesta
- True
- False
Pregunta 29
Pregunta
Prefoldin is ATP dependent
Respuesta
- True
- False
Pregunta 30
Pregunta
Prefolding is a...
Respuesta
-
Dimer
-
Tetramer
-
Hexamer
Pregunta 31
Pregunta
What is the role of Preofoldin in Archea?
Respuesta
-
Captures nascent polypeptides and binds them to Gro-El
-
Captures nascent polypeptides and binds them to CCT
-
Captures nascent polypeptides and binds them to SSA
Pregunta 32
Pregunta
In Eukaryotes, prefoldin might be specific for the folding of what?
Respuesta
-
Actin/tubulin
-
CCT
-
Hsp40
-
Hsp70
Pregunta 33
Pregunta
In archea there are 6 Prefoldin genes (Grim1-6) in Eukaryotes there are 2 (Gimalpha and Gimbeta)
Respuesta
- True
- False
Pregunta 34
Pregunta
Which of the following describe Prefoldin
Respuesta
-
Jellyfish like structure
-
With 6 coiled-coil beta sheets
-
With 6 coiled-coil alpha helices
Pregunta 35
Pregunta
There are 3.5 amino acid residues per helical turn of the coiled coil helices in Prefoldin (not 3.6)
Respuesta
- True
- False
Pregunta 36
Pregunta
The subunits forming the dimerisation interface of the below molecule are what?
Image:
Prefolding (image/png)
Respuesta
-
Hydrophobic
-
Hydrophillic
Pregunta 37
Pregunta
Where do the non-native proteins bind to Prefoldin?
Respuesta
-
Exposed hydrophobic residues in the distal ends of the 'tentacle'
-
Exposed hydrophobic residues in the proximal ends of the 'tentacle'
-
Exposed hydrophobic residues at the top of the 'tentacle'
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