457080
Descripción
Test por gina_evans0312, actualizado hace más de 1 año
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Creado por gina_evans0312
hace casi 11 años
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Pregunta 1
Pregunta
A radioactive probe can take what forms?
Respuesta
-
ssDNA
-
RNA
-
Denatured dsDNA
-
Oligonucleotides
Pregunta 2
Pregunta
Probes can be
Respuesta
-
Radioactive
-
Fluorescent
-
Use Ig's
Pregunta 3
Pregunta
Name the two ways probes can be designed
Respuesta
-
Guesswork
-
Codon Bias
Pregunta 4
Pregunta
What is codon bias?
Respuesta
-
Where cells prefer one codon of an aa to another
-
Where cells will reject certain codons due to what they contain
-
Where some cells can only make tRNA's for certain codons
Pregunta 5
Pregunta
80% of Phe codons are TTC, 20% are TTT
Respuesta
- True
- False
Pregunta 6
Pregunta
If the protein is an enzyme it can be found by adding the substrate
Respuesta
- True
- False
Pregunta 7
Pregunta
State the possible forms of Ig's for protein separation?
Respuesta
-
Immobalised on a column
-
Removable from solution
Pregunta 8
Pregunta
Probes needn't be 100% accurate to work
Respuesta
- True
- False
Pregunta 9
Pregunta
For a Western Blot, how is the DNA stuck to the membrane?
Respuesta
-
Denatured DNA sticks to membrane
-
Bacteria are lysed to release DNA
-
DNA washes off
Pregunta 10
Pregunta
Once the DNA has been bound, what happens next?
Respuesta
-
A probe is added
-
The membrane is run through a gel
-
The membrane is stained
Pregunta 11
Pregunta
In an expression vector, where is the new gene added?
Respuesta
-
The Multiple Cloning Site
-
The Promotor Region
-
Next to the origin
Pregunta 12
Respuesta
-
Origin
-
Antibiotic Resistance
-
Promoter
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Terminator
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Multiple Cloning Site
Pregunta 13
Pregunta
More compliated vectors have proteins tagged for purification
Respuesta
- True
- False
Pregunta 14
Pregunta
What is involved in His Chain Purification?
Respuesta
-
The addition of a His chain to the N terminus
-
The removal of a His chain from the protein
-
The use of a His chain to cut unwatned proteins
Pregunta 15
Pregunta
His Purification uses Histadine's affinity for what metal?
Respuesta
-
Ni2+
-
Fe2+
-
Na+
Pregunta 16
Pregunta
His-tagged proteins will bind to Nickel in a column. Others that don't bind will be washed off.
Respuesta
- True
- False
Pregunta 17
Pregunta
The PET system involves using a promoter for the protein that you can control the expression of
Respuesta
- True
- False
Pregunta 18
Pregunta
What promoter is used in the PET system to control protein expression?
Respuesta
-
T7
-
T8
-
T9
Pregunta 19
Pregunta
In the PET system, what promoter is used to control the T_ expression?
Respuesta
-
Lac promoter
-
Slo Promoter
-
Pap promoter
Pregunta 20
Pregunta
What is required for a good shuttle vector?
Respuesta
-
Replicable in both hosts
-
Selectable in both hosts
-
Expressable in both hosts
Pregunta 21
Pregunta
Shuttle vectors are usually plasmids with additional sequences
Respuesta
- True
- False
Pregunta 22
Pregunta
Shuttle vectors for yeast/fungi need
Respuesta
-
Special origin
-
Antibiotic/metabolic marker
Pregunta 23
Pregunta
A baclovirus would be used as a vector into what?
Respuesta
-
Insects
-
Plants
-
Mammals
Pregunta 24
Pregunta
Secretion of certain proteins can be caused by what?
Respuesta
-
Disulphide bonds
-
Acetylation
-
Methylation
Pregunta 25
Respuesta
-
One for the yeast to use
-
One for the bacteria to use
Pregunta 26
Respuesta
-
URA3- allows growth in the absence of Uracil
-
TRA3- allows growth in the absence of thymidine
-
GRA3- allows growth in the absence of guanine
Pregunta 27
Pregunta
Like bacteria, yeast cannot take up linear DNA
Respuesta
- True
- False
Pregunta 28
Pregunta
What does a YAC contain that a plasmid does not?
Respuesta
-
Non-useful genes
-
Yeast centromere
-
Yeast telomeres
Pregunta 29
Pregunta
Yeast can allow DNA to be directly inserted into its genome
Respuesta
- True
- False
Pregunta 30
Pregunta
How can yeast accept DNA into its genome?
Respuesta
-
Homologous recombination
-
Non-homologous end joining
-
Direct injection
Pregunta 31
Pregunta
In order to directly insert a gene, you must flank the gene with identical stretches of the part of the genome you want to insert it in
Respuesta
- True
- False
Pregunta 32
Pregunta
Plants would use an aglobacterium T1 plasmid
Respuesta
- True
- False
Pregunta 33
Pregunta
How do you replicate transformed plant cells?
Respuesta
-
Grown them as plantlets
-
Clone them
-
Asexually reproduce them
Pregunta 34
Pregunta
Mammal shuttle vectors are the same as yeast vectors, but the yeast origin is replaced with what?
Respuesta
-
An SV40 promoter
-
A UG35 promoter
-
A FT12 promoter
Pregunta 35
Pregunta
Retroviruses can also be used to insert transgenes into mammilian genomes by packing the transgene in a retroviral capsule
Respuesta
- True
- False
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