magnification= size of image / size of real object
magnification= size of real object / size of image
Pregunta 2
Pregunta
Before cell fractionation what is the sample put in?
Respuesta
A cold, buffered solution with a similar water potential to the sample
Nothing
A warmed solution with a high pH
Pregunta 3
Pregunta
What is homogenation?
Respuesta
The preparation of the sample for cell fractionation
The breaking down of cells in a homogeniser
The removal of foreign objects in a sample
Pregunta 4
Pregunta
What happens in Ultracentrifugation?
Respuesta
The cell fragments are separated in a centrifuge
Fragmentation of cells
Pregunta 5
Pregunta
At what speed are nuclei, mitochondria and lysosomes separated out?
Respuesta
Nuclei- 3500 min-1
Mitochondria- 16500 min-1
Lysosomes- 1000 min-1
Nuclei- 1000 min-1
Mitochondria- 3500 min-1
Lysosomes- 16500 min-1
Pregunta 6
Pregunta
Transmission electron microscope (TEM)
Respuesta
A thin sample is used to create a photomicrograph-not in colour. A photomicrograph is a 2D flat image due to the thinness of the sample and it has a resolution of 0.1 nanometres.
Sample does not need to be too thin as electrons don't penetrate. Creates a 3D image which can be coloured and it has a resolution of 20 nanometres.